Dose dependent rhizospheric Ni toxicity evaluation: Membrane stability and antioxidant potential of Vigna species

Different concentrations of Ni are found in agricultural soil released from various environmental sources. The responses of plants grown in such soil-substratum vary accordingly depending upon the concentration of metal present and plant species. To assess the toxic effects of Ni and extent of plant...

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Autores principales:	Mahmood,Seema, Ishtiaq,Shabnam, Yasin,Ghulam, Irshad,Ahsan
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2016
Materias:	Antioxidant defense catalasa malondialdehyde (MDA) peroxidases ROS
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392016000300017
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spelling	oai:scielo:S0718-583920160003000172018-10-01Dose dependent rhizospheric Ni toxicity evaluation: Membrane stability and antioxidant potential of Vigna speciesMahmood,SeemaIshtiaq,ShabnamYasin,GhulamIrshad,Ahsan Antioxidant defense catalasa malondialdehyde (MDA) peroxidases ROS Different concentrations of Ni are found in agricultural soil released from various environmental sources. The responses of plants grown in such soil-substratum vary accordingly depending upon the concentration of metal present and plant species. To assess the toxic effects of Ni and extent of plant defensive strategies, increasing Ni doses (50, 100, and 150 mg kg-1) were used in sandy-loam soil-substratum at pH 7.9 to evaluate the performance of Vigna species (V. cylindrica [L.] Skeels, V. mungo [L.] Hepper, and V radiata [L.] R. Wilczek). The experiment was conducted in a complete randomized design. Nickel stress was induced by adding various concentrations of Ni chloride in soil substratum. Malondialdehyde (MDA) and antioxidant levels were determined in roots and leaves. Escalating levels of Ni in soil resulted in an affirmative relationship between MDA with that of antioxidants. A dose-dependent increase in the activity of Superoxide Dismutases (SOD), Catalase (CAT), and peroxidases (POD) suggested the existence of a sequence response of these enzymes to scavenge oxidative stress in the roots. However, inadequate production of SOD and CAT appeared to be compensated by the enhanced activity of POD, which acted as potent quencher to reactive oxygen species (ROS) in leaves. At the most elevated Ni dose, SOD, CAT and POD activities were insufficient to counteract ROS generated that led to membrane damage manifested by elevated MDA levels. Nevertheless, SOD and CAT alleviated Ni toxicity in roots while SOD, CAT and POD acted in a concurrent manner to protect leaves from oxidative damage in V. cylindrica. The study clearly indicated a Ni dependent antioxidant enzymes defense system in V. cylindrica.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.76 n.3 20162016-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392016000300017en10.4067/S0718-58392016000300017
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Antioxidant defense catalasa malondialdehyde (MDA) peroxidases ROS
spellingShingle	Antioxidant defense catalasa malondialdehyde (MDA) peroxidases ROS Mahmood,Seema Ishtiaq,Shabnam Yasin,Ghulam Irshad,Ahsan Dose dependent rhizospheric Ni toxicity evaluation: Membrane stability and antioxidant potential of Vigna species
description	Different concentrations of Ni are found in agricultural soil released from various environmental sources. The responses of plants grown in such soil-substratum vary accordingly depending upon the concentration of metal present and plant species. To assess the toxic effects of Ni and extent of plant defensive strategies, increasing Ni doses (50, 100, and 150 mg kg-1) were used in sandy-loam soil-substratum at pH 7.9 to evaluate the performance of Vigna species (V. cylindrica [L.] Skeels, V. mungo [L.] Hepper, and V radiata [L.] R. Wilczek). The experiment was conducted in a complete randomized design. Nickel stress was induced by adding various concentrations of Ni chloride in soil substratum. Malondialdehyde (MDA) and antioxidant levels were determined in roots and leaves. Escalating levels of Ni in soil resulted in an affirmative relationship between MDA with that of antioxidants. A dose-dependent increase in the activity of Superoxide Dismutases (SOD), Catalase (CAT), and peroxidases (POD) suggested the existence of a sequence response of these enzymes to scavenge oxidative stress in the roots. However, inadequate production of SOD and CAT appeared to be compensated by the enhanced activity of POD, which acted as potent quencher to reactive oxygen species (ROS) in leaves. At the most elevated Ni dose, SOD, CAT and POD activities were insufficient to counteract ROS generated that led to membrane damage manifested by elevated MDA levels. Nevertheless, SOD and CAT alleviated Ni toxicity in roots while SOD, CAT and POD acted in a concurrent manner to protect leaves from oxidative damage in V. cylindrica. The study clearly indicated a Ni dependent antioxidant enzymes defense system in V. cylindrica.
author	Mahmood,Seema Ishtiaq,Shabnam Yasin,Ghulam Irshad,Ahsan
author_facet	Mahmood,Seema Ishtiaq,Shabnam Yasin,Ghulam Irshad,Ahsan
author_sort	Mahmood,Seema
title	Dose dependent rhizospheric Ni toxicity evaluation: Membrane stability and antioxidant potential of Vigna species
title_short	Dose dependent rhizospheric Ni toxicity evaluation: Membrane stability and antioxidant potential of Vigna species
title_full	Dose dependent rhizospheric Ni toxicity evaluation: Membrane stability and antioxidant potential of Vigna species
title_fullStr	Dose dependent rhizospheric Ni toxicity evaluation: Membrane stability and antioxidant potential of Vigna species
title_full_unstemmed	Dose dependent rhizospheric Ni toxicity evaluation: Membrane stability and antioxidant potential of Vigna species
title_sort	dose dependent rhizospheric ni toxicity evaluation: membrane stability and antioxidant potential of vigna species
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392016000300017
work_keys_str_mv	AT mahmoodseema dosedependentrhizosphericnitoxicityevaluationmembranestabilityandantioxidantpotentialofvignaspecies AT ishtiaqshabnam dosedependentrhizosphericnitoxicityevaluationmembranestabilityandantioxidantpotentialofvignaspecies AT yasinghulam dosedependentrhizosphericnitoxicityevaluationmembranestabilityandantioxidantpotentialofvignaspecies AT irshadahsan dosedependentrhizosphericnitoxicityevaluationmembranestabilityandantioxidantpotentialofvignaspecies
_version_	1714205363037798400

Dose dependent rhizospheric Ni toxicity evaluation: Membrane stability and antioxidant potential of Vigna species

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