Conditions to optimize mass production of Metarhizium anisopliae (Metschn.) Sorokin 1883 in different substrates

One of the best alternatives to reduce the amount of chemical insecticides released into the environment is biological agents. Metarhizium anisopliae (Metschn.) Sorokin 1883 (Hypocreales: Clavicipitaceae) is an entomopathogenic fungus with great potential as a biological pesticide to biologically co...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Barra-Bucarei,Lorena, Vergara,Pedro, Cortes,Amparo
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2016
Materias:	Biological control entomopathogenic fungi mass production optimization response surface methodology
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392016000400008
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-58392016000400008
record_format	dspace
spelling	oai:scielo:S0718-583920160004000082018-10-01Conditions to optimize mass production of Metarhizium anisopliae (Metschn.) Sorokin 1883 in different substratesBarra-Bucarei,LorenaVergara,PedroCortes,Amparo Biological control entomopathogenic fungi mass production optimization response surface methodology One of the best alternatives to reduce the amount of chemical insecticides released into the environment is biological agents. Metarhizium anisopliae (Metschn.) Sorokin 1883 (Hypocreales: Clavicipitaceae) is an entomopathogenic fungus with great potential as a biological pesticide to biologically control pests. However, the relatively high cost of the substrate needed for its mass production system increases product price and discourages its use. The objective of this study was to optimize the mass production conditions of M. anisopliae for use as a biological control agent using two solid substrates, new parboiled rice (NPR) and recycled parboiled rice (RPR). Conidial production was optimized by the response surface methodology (RSM). The effects of the temperature, time, and molasses variables and the interactions between them (conidia g-1) were determined. For the NPR substrate, it was determined that the significant variables were time and temperature, and the interactions were temperature x molasses and temperature x time. For the RPR substrate, the significant variables were temperature and time, and the interactions were time x molasses and temperature x time. Both substrates obtained the highest industrial yields at 25 °C for a period of 20 d. Given that the percentage of molasses was not critical for yields, it is recommended that it be set at 5% to reduce costs. Finally, it was possible to use the RPR substrate from the M. anisopliae produ9ction itself as an alternative to solid substrate; mean industrial performance (conidia g-1) was higher than values obtained with NPR and at a lower cost.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.76 n.4 20162016-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392016000400008en10.4067/S0718-58392016000400008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Biological control entomopathogenic fungi mass production optimization response surface methodology
spellingShingle	Biological control entomopathogenic fungi mass production optimization response surface methodology Barra-Bucarei,Lorena Vergara,Pedro Cortes,Amparo Conditions to optimize mass production of Metarhizium anisopliae (Metschn.) Sorokin 1883 in different substrates
description	One of the best alternatives to reduce the amount of chemical insecticides released into the environment is biological agents. Metarhizium anisopliae (Metschn.) Sorokin 1883 (Hypocreales: Clavicipitaceae) is an entomopathogenic fungus with great potential as a biological pesticide to biologically control pests. However, the relatively high cost of the substrate needed for its mass production system increases product price and discourages its use. The objective of this study was to optimize the mass production conditions of M. anisopliae for use as a biological control agent using two solid substrates, new parboiled rice (NPR) and recycled parboiled rice (RPR). Conidial production was optimized by the response surface methodology (RSM). The effects of the temperature, time, and molasses variables and the interactions between them (conidia g-1) were determined. For the NPR substrate, it was determined that the significant variables were time and temperature, and the interactions were temperature x molasses and temperature x time. For the RPR substrate, the significant variables were temperature and time, and the interactions were time x molasses and temperature x time. Both substrates obtained the highest industrial yields at 25 °C for a period of 20 d. Given that the percentage of molasses was not critical for yields, it is recommended that it be set at 5% to reduce costs. Finally, it was possible to use the RPR substrate from the M. anisopliae produ9ction itself as an alternative to solid substrate; mean industrial performance (conidia g-1) was higher than values obtained with NPR and at a lower cost.
author	Barra-Bucarei,Lorena Vergara,Pedro Cortes,Amparo
author_facet	Barra-Bucarei,Lorena Vergara,Pedro Cortes,Amparo
author_sort	Barra-Bucarei,Lorena
title	Conditions to optimize mass production of Metarhizium anisopliae (Metschn.) Sorokin 1883 in different substrates
title_short	Conditions to optimize mass production of Metarhizium anisopliae (Metschn.) Sorokin 1883 in different substrates
title_full	Conditions to optimize mass production of Metarhizium anisopliae (Metschn.) Sorokin 1883 in different substrates
title_fullStr	Conditions to optimize mass production of Metarhizium anisopliae (Metschn.) Sorokin 1883 in different substrates
title_full_unstemmed	Conditions to optimize mass production of Metarhizium anisopliae (Metschn.) Sorokin 1883 in different substrates
title_sort	conditions to optimize mass production of metarhizium anisopliae (metschn.) sorokin 1883 in different substrates
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392016000400008
work_keys_str_mv	AT barrabucareilorena conditionstooptimizemassproductionofmetarhiziumanisopliaemetschnsorokin1883indifferentsubstrates AT vergarapedro conditionstooptimizemassproductionofmetarhiziumanisopliaemetschnsorokin1883indifferentsubstrates AT cortesamparo conditionstooptimizemassproductionofmetarhiziumanisopliaemetschnsorokin1883indifferentsubstrates
_version_	1714205364519436288

Conditions to optimize mass production of Metarhizium anisopliae (Metschn.) Sorokin 1883 in different substrates

Ejemplares similares