Changes in physiological and antioxidant activity of indica rice seedlings in response to mannitol-induced osmotic stress

Morphological, physiological, and biochemical plant responses were studied in unstressed and stressed seedlings of indica rice (Oryza sativa L.) genotypes. The effect of NB medium supplemented with a 100 mM mannitol treatment, which induced drought stress conditions, was measured for relative growth...

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Autor principal:	Chutipaijit,Sutee
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2016
Materias:	ABTS antioxidant enzyme DPPH drought Oryza sativa rice
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392016000400009
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spelling	oai:scielo:S0718-583920160004000092018-10-01Changes in physiological and antioxidant activity of indica rice seedlings in response to mannitol-induced osmotic stressChutipaijit,Sutee ABTS antioxidant enzyme DPPH drought Oryza sativa rice Morphological, physiological, and biochemical plant responses were studied in unstressed and stressed seedlings of indica rice (Oryza sativa L.) genotypes. The effect of NB medium supplemented with a 100 mM mannitol treatment, which induced drought stress conditions, was measured for relative growth rate, cell membrane stability, antioxidant enzyme activity (superoxide dismutase [SOD], catalase [CAT], and peroxidase [POD]), and total antioxidant capacity by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2’-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid] diammonium salt) assays. Results of morphological and physiological factors showed two contrasting rice groups, drought-sensitive and drought-tolerant genotypes. After drought stress, the increased rate of SOD activity was lower in drought-tolerant than in drought-sensitive genotypes, but the increased rate of CAT and POD activity and total antioxidant capacity was higher in drought-tolerant than in drought-sensitive genotypes. Increasing changes in activity levels of antioxidant enzymes and total antioxidant capacity indicated more rapid free radical scavenging compared with relative growth and cell membrane stability in drought-tolerant genotypes under short-term treatment. The trend toward change in CAT and POD antioxidant enzyme activity and total antioxidant capacity of stressed seedlings, as well as the correlation between these changes, and the morphological and physiological responses (0.96 correlation coefficient [R²] between relative growth rate and CAT; 0.93 R² between relative growth rate and POD enzyme activity; 0.96 R² between relative growth rate and Trolox equivalent antioxidant capacity values) coincided with the degree of drought tolerance.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.76 n.4 20162016-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392016000400009en10.4067/S0718-58392016000400009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	ABTS antioxidant enzyme DPPH drought Oryza sativa rice
spellingShingle	ABTS antioxidant enzyme DPPH drought Oryza sativa rice Chutipaijit,Sutee Changes in physiological and antioxidant activity of indica rice seedlings in response to mannitol-induced osmotic stress
description	Morphological, physiological, and biochemical plant responses were studied in unstressed and stressed seedlings of indica rice (Oryza sativa L.) genotypes. The effect of NB medium supplemented with a 100 mM mannitol treatment, which induced drought stress conditions, was measured for relative growth rate, cell membrane stability, antioxidant enzyme activity (superoxide dismutase [SOD], catalase [CAT], and peroxidase [POD]), and total antioxidant capacity by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2’-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid] diammonium salt) assays. Results of morphological and physiological factors showed two contrasting rice groups, drought-sensitive and drought-tolerant genotypes. After drought stress, the increased rate of SOD activity was lower in drought-tolerant than in drought-sensitive genotypes, but the increased rate of CAT and POD activity and total antioxidant capacity was higher in drought-tolerant than in drought-sensitive genotypes. Increasing changes in activity levels of antioxidant enzymes and total antioxidant capacity indicated more rapid free radical scavenging compared with relative growth and cell membrane stability in drought-tolerant genotypes under short-term treatment. The trend toward change in CAT and POD antioxidant enzyme activity and total antioxidant capacity of stressed seedlings, as well as the correlation between these changes, and the morphological and physiological responses (0.96 correlation coefficient [R²] between relative growth rate and CAT; 0.93 R² between relative growth rate and POD enzyme activity; 0.96 R² between relative growth rate and Trolox equivalent antioxidant capacity values) coincided with the degree of drought tolerance.
author	Chutipaijit,Sutee
author_facet	Chutipaijit,Sutee
author_sort	Chutipaijit,Sutee
title	Changes in physiological and antioxidant activity of indica rice seedlings in response to mannitol-induced osmotic stress
title_short	Changes in physiological and antioxidant activity of indica rice seedlings in response to mannitol-induced osmotic stress
title_full	Changes in physiological and antioxidant activity of indica rice seedlings in response to mannitol-induced osmotic stress
title_fullStr	Changes in physiological and antioxidant activity of indica rice seedlings in response to mannitol-induced osmotic stress
title_full_unstemmed	Changes in physiological and antioxidant activity of indica rice seedlings in response to mannitol-induced osmotic stress
title_sort	changes in physiological and antioxidant activity of indica rice seedlings in response to mannitol-induced osmotic stress
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392016000400009
work_keys_str_mv	AT chutipaijitsutee changesinphysiologicalandantioxidantactivityofindicariceseedlingsinresponsetomannitolinducedosmoticstress
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Changes in physiological and antioxidant activity of indica rice seedlings in response to mannitol-induced osmotic stress

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