An efficient method for in vitro propagation of Alstroemeria pallida Graham rhizomes

ABSTRACT Alstroemeria is a genus native to South America and commercially has commonly been propagated vegetatively by rhizome division, with low efficiency, high time consumption and a high risk of virus dissemination. In vitro propagation has several advantages, particularly in terms of efficiency...

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Autores principales:	Aros,Danilo, Vásquez,Marko, Rivas,Constanza, Prat,Maria Loreto
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2017
Materias:	Alstroemeria cytokinins agar tissue culture and micropropagation.
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392017000100012
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spelling	oai:scielo:S0718-583920170001000122017-11-09An efficient method for in vitro propagation of Alstroemeria pallida Graham rhizomesAros,DaniloVásquez,MarkoRivas,ConstanzaPrat,Maria Loreto Alstroemeria cytokinins agar tissue culture and micropropagation. ABSTRACT Alstroemeria is a genus native to South America and commercially has commonly been propagated vegetatively by rhizome division, with low efficiency, high time consumption and a high risk of virus dissemination. In vitro propagation has several advantages, particularly in terms of efficiency and has been applied to the micropropagation of alstroemeria. This study aims to describe an efficient method for the in vitro propagation of Alstroemeria pallida Graham, a Chilean native species of high ornamental value. Concentrations of agar (0.0, 3.5 and 7.0 g L-1) and 6-benzylaminopurine (BAP) (0.0, 0.5, 1.0 and 2.0 mg L-1) were supplemented with MS culture medium to evaluate explant weight (g), rhizome length (cm), shoot length (cm) and proliferation rate. The highest explant weight was observed in rhizomes grown in culture medium supplemented with 3.5 g L-1 agar (3.79 g), and treatments using 2.0 g L-1 BAP showed the highest weight increase (3.33 g) after 8 wk. The proliferation rate rose with increasing concentrations of BAP, whereas low concentrations of BAP promoted longer shoots. An efficient method for in vitro propagation of A. pallida rhizomes was described, which could be useful for its conservation, domestication and further breeding.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.77 n.1 20172017-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392017000100012en10.4067/S0718-58392017000100012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Alstroemeria cytokinins agar tissue culture and micropropagation.
spellingShingle	Alstroemeria cytokinins agar tissue culture and micropropagation. Aros,Danilo Vásquez,Marko Rivas,Constanza Prat,Maria Loreto An efficient method for in vitro propagation of Alstroemeria pallida Graham rhizomes
description	ABSTRACT Alstroemeria is a genus native to South America and commercially has commonly been propagated vegetatively by rhizome division, with low efficiency, high time consumption and a high risk of virus dissemination. In vitro propagation has several advantages, particularly in terms of efficiency and has been applied to the micropropagation of alstroemeria. This study aims to describe an efficient method for the in vitro propagation of Alstroemeria pallida Graham, a Chilean native species of high ornamental value. Concentrations of agar (0.0, 3.5 and 7.0 g L-1) and 6-benzylaminopurine (BAP) (0.0, 0.5, 1.0 and 2.0 mg L-1) were supplemented with MS culture medium to evaluate explant weight (g), rhizome length (cm), shoot length (cm) and proliferation rate. The highest explant weight was observed in rhizomes grown in culture medium supplemented with 3.5 g L-1 agar (3.79 g), and treatments using 2.0 g L-1 BAP showed the highest weight increase (3.33 g) after 8 wk. The proliferation rate rose with increasing concentrations of BAP, whereas low concentrations of BAP promoted longer shoots. An efficient method for in vitro propagation of A. pallida rhizomes was described, which could be useful for its conservation, domestication and further breeding.
author	Aros,Danilo Vásquez,Marko Rivas,Constanza Prat,Maria Loreto
author_facet	Aros,Danilo Vásquez,Marko Rivas,Constanza Prat,Maria Loreto
author_sort	Aros,Danilo
title	An efficient method for in vitro propagation of Alstroemeria pallida Graham rhizomes
title_short	An efficient method for in vitro propagation of Alstroemeria pallida Graham rhizomes
title_full	An efficient method for in vitro propagation of Alstroemeria pallida Graham rhizomes
title_fullStr	An efficient method for in vitro propagation of Alstroemeria pallida Graham rhizomes
title_full_unstemmed	An efficient method for in vitro propagation of Alstroemeria pallida Graham rhizomes
title_sort	efficient method for in vitro propagation of alstroemeria pallida graham rhizomes
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392017000100012
work_keys_str_mv	AT arosdanilo anefficientmethodforinvitropropagationofalstroemeriapallidagrahamrhizomes AT vasquezmarko anefficientmethodforinvitropropagationofalstroemeriapallidagrahamrhizomes AT rivasconstanza anefficientmethodforinvitropropagationofalstroemeriapallidagrahamrhizomes AT pratmarialoreto anefficientmethodforinvitropropagationofalstroemeriapallidagrahamrhizomes AT arosdanilo efficientmethodforinvitropropagationofalstroemeriapallidagrahamrhizomes AT vasquezmarko efficientmethodforinvitropropagationofalstroemeriapallidagrahamrhizomes AT rivasconstanza efficientmethodforinvitropropagationofalstroemeriapallidagrahamrhizomes AT pratmarialoreto efficientmethodforinvitropropagationofalstroemeriapallidagrahamrhizomes
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An efficient method for in vitro propagation of Alstroemeria pallida Graham rhizomes

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