Multiple resistance to ACCase and ALS-inhibiting herbicides in Beckmannia syzigachne (Steud.) Fernald without mutations in the target enzymes

ABSTRACT American slough grass (Beckmannia syzigachne [Steud.] Fernald) is a worldwide weed and is widely distributed in rice-wheat rotations in China. Fenoxaprop-P-ethyl and mesosulfuron-methyl are two major herbicides used to control B. syzigachne. Resistance has evolved in B. syzigachne under con...

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Autores principales:	Li,Wei, Zhang,Lele, Zhao,Ning, Guo,Wenlei, Liu,Weitang, Li,Lingxu, Wang,Jinxin
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2017
Materias:	Herbicide resistance metabolic resistance P450 inhibitors target enzymes sequencing.
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392017000300257
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spelling	oai:scielo:S0718-583920170003002572017-11-09Multiple resistance to ACCase and ALS-inhibiting herbicides in Beckmannia syzigachne (Steud.) Fernald without mutations in the target enzymesLi,WeiZhang,LeleZhao,NingGuo,WenleiLiu,WeitangLi,LingxuWang,Jinxin Herbicide resistance metabolic resistance P450 inhibitors target enzymes sequencing. ABSTRACT American slough grass (Beckmannia syzigachne [Steud.] Fernald) is a worldwide weed and is widely distributed in rice-wheat rotations in China. Fenoxaprop-P-ethyl and mesosulfuron-methyl are two major herbicides used to control B. syzigachne. Resistance has evolved in B. syzigachne under continuous selective pressure from herbicides. This study aimed to establish the cross-resistance pattern of a resistant population and explore the potential non-target-site based resistance mechanisms of B. syzigachne. Sequencing of target enzyme genes (acetyl coenzyme A carboxylase [ACCase] and acetolactate synthase [ALS]) revealed that there were no resistance-endowing amino acid substitutions in the resistant B. syzigachne population (RF1) compared with the sensitive population (SF1), and obtained the purified materials. Furthermore, piperonyl butoxide (PBO) and malathion showed synergistic effects with fenoxaprop-P-ethyl and mesosulfuron-methyl respectively in RF1. Therefore, we speculate that the resistance observed in B. syzigachne was related with metabolic, mostly involving the cytochrome P450 enzymes. Cross resistance patterns showed that the purified resistant B. syzigachne produce high resistance to fenoxaprop-P-ethyl and pyroxsulam; intermediate resistance to flucarbazone-sodium; low resistance to quizalofop-P-ethyl, clodinafop-propargyl, sethoxydim and mesosulfuron-methyl; sensitive to clethodim pinoxaden and isoproturon.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.77 n.3 20172017-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392017000300257en10.4067/S0718-58392017000300257
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Herbicide resistance metabolic resistance P450 inhibitors target enzymes sequencing.
spellingShingle	Herbicide resistance metabolic resistance P450 inhibitors target enzymes sequencing. Li,Wei Zhang,Lele Zhao,Ning Guo,Wenlei Liu,Weitang Li,Lingxu Wang,Jinxin Multiple resistance to ACCase and ALS-inhibiting herbicides in Beckmannia syzigachne (Steud.) Fernald without mutations in the target enzymes
description	ABSTRACT American slough grass (Beckmannia syzigachne [Steud.] Fernald) is a worldwide weed and is widely distributed in rice-wheat rotations in China. Fenoxaprop-P-ethyl and mesosulfuron-methyl are two major herbicides used to control B. syzigachne. Resistance has evolved in B. syzigachne under continuous selective pressure from herbicides. This study aimed to establish the cross-resistance pattern of a resistant population and explore the potential non-target-site based resistance mechanisms of B. syzigachne. Sequencing of target enzyme genes (acetyl coenzyme A carboxylase [ACCase] and acetolactate synthase [ALS]) revealed that there were no resistance-endowing amino acid substitutions in the resistant B. syzigachne population (RF1) compared with the sensitive population (SF1), and obtained the purified materials. Furthermore, piperonyl butoxide (PBO) and malathion showed synergistic effects with fenoxaprop-P-ethyl and mesosulfuron-methyl respectively in RF1. Therefore, we speculate that the resistance observed in B. syzigachne was related with metabolic, mostly involving the cytochrome P450 enzymes. Cross resistance patterns showed that the purified resistant B. syzigachne produce high resistance to fenoxaprop-P-ethyl and pyroxsulam; intermediate resistance to flucarbazone-sodium; low resistance to quizalofop-P-ethyl, clodinafop-propargyl, sethoxydim and mesosulfuron-methyl; sensitive to clethodim pinoxaden and isoproturon.
author	Li,Wei Zhang,Lele Zhao,Ning Guo,Wenlei Liu,Weitang Li,Lingxu Wang,Jinxin
author_facet	Li,Wei Zhang,Lele Zhao,Ning Guo,Wenlei Liu,Weitang Li,Lingxu Wang,Jinxin
author_sort	Li,Wei
title	Multiple resistance to ACCase and ALS-inhibiting herbicides in Beckmannia syzigachne (Steud.) Fernald without mutations in the target enzymes
title_short	Multiple resistance to ACCase and ALS-inhibiting herbicides in Beckmannia syzigachne (Steud.) Fernald without mutations in the target enzymes
title_full	Multiple resistance to ACCase and ALS-inhibiting herbicides in Beckmannia syzigachne (Steud.) Fernald without mutations in the target enzymes
title_fullStr	Multiple resistance to ACCase and ALS-inhibiting herbicides in Beckmannia syzigachne (Steud.) Fernald without mutations in the target enzymes
title_full_unstemmed	Multiple resistance to ACCase and ALS-inhibiting herbicides in Beckmannia syzigachne (Steud.) Fernald without mutations in the target enzymes
title_sort	multiple resistance to accase and als-inhibiting herbicides in beckmannia syzigachne (steud.) fernald without mutations in the target enzymes
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392017000300257
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Multiple resistance to ACCase and ALS-inhibiting herbicides in Beckmannia syzigachne (Steud.) Fernald without mutations in the target enzymes

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