Genetic and biological characterization of Macrophomina phaseolina (Tassi) Goid. causing crown and root rot of strawberry

ABSTRACT In recent years, crown and root rot of strawberry (Fragaria ×ananassa Duchesne ex Rozier) caused by Macrophomina phaseolina (Tassi) Goid. has affected strawberry production areas worldwide, and in developed countries its emergency has been attributed to the replacement of methyl bromide. Th...

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Autores principales:	Sánchez,Soledad, Chamorro,Manuel, Henríquez,José L., Grez,Javiera, Díaz,Isabel, Santos,Berta de los, Gambardella,Marina
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2017
Materias:	Charcoal rot chlorate sensitivity Fragaria ×ananassa genetic diversity microsatellite marker mycelial growth optimal temperature SSR.
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392017000400325
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spelling	oai:scielo:S0718-583920170004003252018-01-30Genetic and biological characterization of Macrophomina phaseolina (Tassi) Goid. causing crown and root rot of strawberrySánchez,SoledadChamorro,ManuelHenríquez,José L.Grez,JavieraDíaz,IsabelSantos,Berta de losGambardella,Marina Charcoal rot chlorate sensitivity Fragaria ×ananassa genetic diversity microsatellite marker mycelial growth optimal temperature SSR. ABSTRACT In recent years, crown and root rot of strawberry (Fragaria ×ananassa Duchesne ex Rozier) caused by Macrophomina phaseolina (Tassi) Goid. has affected strawberry production areas worldwide, and in developed countries its emergency has been attributed to the replacement of methyl bromide. The disease was reported in strawberry crop in Chile in 2013, in fields without fumigation. The use of resistant cultivars rises as an alternative to the management of this disease. The objective of this study was to perform a biological and molecular characterization of isolates obtained from two growing regions in Chile and Spain. A total of 35 isolates were characterized for mycelial growth at different temperatures and for chlorate sensitivity. Seven simple sequence repeat loci were used for genetic characterization. Differences were found between Chilean and Spanish isolates in both characterizations. The optimal temperature for mycelial growth was lower in Chilean than in Spanish isolates (30 and 35 °C, respectively). Meanwhile, Chilean isolates were more sensitive to chlorate. In terms of genetic characterization, Polymorphism Information Content (PIC) ranged from 0.38 to 0.85, two main groups were identified, the first group included Spanish isolates and the second group corresponded to Chilean isolates, results were supported by a population structure analysis. This study determined clear differences between two populations of Chilean and Spanish M. phaseolina isolates as causal agent of crown and root rot of strawberry.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.77 n.4 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392017000400325en10.4067/S0718-58392017000400325
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Charcoal rot chlorate sensitivity Fragaria ×ananassa genetic diversity microsatellite marker mycelial growth optimal temperature SSR.
spellingShingle	Charcoal rot chlorate sensitivity Fragaria ×ananassa genetic diversity microsatellite marker mycelial growth optimal temperature SSR. Sánchez,Soledad Chamorro,Manuel Henríquez,José L. Grez,Javiera Díaz,Isabel Santos,Berta de los Gambardella,Marina Genetic and biological characterization of Macrophomina phaseolina (Tassi) Goid. causing crown and root rot of strawberry
description	ABSTRACT In recent years, crown and root rot of strawberry (Fragaria ×ananassa Duchesne ex Rozier) caused by Macrophomina phaseolina (Tassi) Goid. has affected strawberry production areas worldwide, and in developed countries its emergency has been attributed to the replacement of methyl bromide. The disease was reported in strawberry crop in Chile in 2013, in fields without fumigation. The use of resistant cultivars rises as an alternative to the management of this disease. The objective of this study was to perform a biological and molecular characterization of isolates obtained from two growing regions in Chile and Spain. A total of 35 isolates were characterized for mycelial growth at different temperatures and for chlorate sensitivity. Seven simple sequence repeat loci were used for genetic characterization. Differences were found between Chilean and Spanish isolates in both characterizations. The optimal temperature for mycelial growth was lower in Chilean than in Spanish isolates (30 and 35 °C, respectively). Meanwhile, Chilean isolates were more sensitive to chlorate. In terms of genetic characterization, Polymorphism Information Content (PIC) ranged from 0.38 to 0.85, two main groups were identified, the first group included Spanish isolates and the second group corresponded to Chilean isolates, results were supported by a population structure analysis. This study determined clear differences between two populations of Chilean and Spanish M. phaseolina isolates as causal agent of crown and root rot of strawberry.
author	Sánchez,Soledad Chamorro,Manuel Henríquez,José L. Grez,Javiera Díaz,Isabel Santos,Berta de los Gambardella,Marina
author_facet	Sánchez,Soledad Chamorro,Manuel Henríquez,José L. Grez,Javiera Díaz,Isabel Santos,Berta de los Gambardella,Marina
author_sort	Sánchez,Soledad
title	Genetic and biological characterization of Macrophomina phaseolina (Tassi) Goid. causing crown and root rot of strawberry
title_short	Genetic and biological characterization of Macrophomina phaseolina (Tassi) Goid. causing crown and root rot of strawberry
title_full	Genetic and biological characterization of Macrophomina phaseolina (Tassi) Goid. causing crown and root rot of strawberry
title_fullStr	Genetic and biological characterization of Macrophomina phaseolina (Tassi) Goid. causing crown and root rot of strawberry
title_full_unstemmed	Genetic and biological characterization of Macrophomina phaseolina (Tassi) Goid. causing crown and root rot of strawberry
title_sort	genetic and biological characterization of macrophomina phaseolina (tassi) goid. causing crown and root rot of strawberry
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392017000400325
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Genetic and biological characterization of Macrophomina phaseolina (Tassi) Goid. causing crown and root rot of strawberry

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