Analysis of rhamnogalacturonan I fragments as elicitors of the defense mechanism in tomato fruit

ABSTRACT The rhamnogalacturonan I (RGI) is part of pectin plant cell wall, and currently there is scarce information about the elicitor effect of its fragments in the fruit defense mechanism. In this work, the effect of the fragments of RGI on the defense mechanism in tomato (Solanum lycopersicum L....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jiménez-Maldonado,María Isabel, Tiznado-Hernández,Martín Ernesto, Rascón-Chu,Agustín, Carvajal-Millán,Elizabeth, Lizardi-Mendoza,Jaime, Troncoso-Rojas,Rosalba
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2018
Materias:	Fragments of rhamnogalacturonan I FT-IR induction of PR proteins light scattering Solanum lycopersicum sugar profile tomato fruits.
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392018000300339
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-58392018000300339
record_format	dspace
spelling	oai:scielo:S0718-583920180003003392018-11-14Analysis of rhamnogalacturonan I fragments as elicitors of the defense mechanism in tomato fruitJiménez-Maldonado,María IsabelTiznado-Hernández,Martín ErnestoRascón-Chu,AgustínCarvajal-Millán,ElizabethLizardi-Mendoza,JaimeTroncoso-Rojas,Rosalba Fragments of rhamnogalacturonan I FT-IR induction of PR proteins light scattering Solanum lycopersicum sugar profile tomato fruits. ABSTRACT The rhamnogalacturonan I (RGI) is part of pectin plant cell wall, and currently there is scarce information about the elicitor effect of its fragments in the fruit defense mechanism. In this work, the effect of the fragments of RGI on the defense mechanism in tomato (Solanum lycopersicum L.) fruits was evaluated. Three fragments of RGI released from enzymatic degradation by RG lyase were characterized according to their physical-chemical and structural characteristics. Two of RGI fragments (F-1 and F-2) were selected and applied on tomato fruits during 48 h. At different exposure times (0, 0.5, 1, 6, 24, and 48 h) tomato samples were taken, and the enzymatic activity of chitinase was assessed by fluorometry, and β-1,3-glucanase and peroxidase by spectrophotometry (UV-visible). The absorption bands determined by Fourier transform infra-red (FT-IR) spectroscopy in the RGI fragments, was different concerning RGI no fragmented. The RGI had a molecular weight of 64 kDa and an intrinsic viscosity of 40.9 mL g-1; while the molecular weight of F-1 and F-2 fragments were 20 and 16 kDa, and an average intrinsic viscosity of 27 and 26 mL g-1, respectively. The content of galacturonic acid decreased, and the neutral sugars increased concerning the time of enzymatic degradation. F-1 and F-2 fragments induced an increase in the level of β -1,3-glucanase activity in tomato fruits; however, an increase in the enzymatic activity of chitinase and peroxidase was observed after 0.5 h of exposure to F-2 fragment. It was concluded that the RGI fragments were structurally different from the RGI no fragmented and induced the natural defense mechanism of the tomato fruit.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.78 n.3 20182018-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392018000300339en10.4067/S0718-58392018000300339
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Fragments of rhamnogalacturonan I FT-IR induction of PR proteins light scattering Solanum lycopersicum sugar profile tomato fruits.
spellingShingle	Fragments of rhamnogalacturonan I FT-IR induction of PR proteins light scattering Solanum lycopersicum sugar profile tomato fruits. Jiménez-Maldonado,María Isabel Tiznado-Hernández,Martín Ernesto Rascón-Chu,Agustín Carvajal-Millán,Elizabeth Lizardi-Mendoza,Jaime Troncoso-Rojas,Rosalba Analysis of rhamnogalacturonan I fragments as elicitors of the defense mechanism in tomato fruit
description	ABSTRACT The rhamnogalacturonan I (RGI) is part of pectin plant cell wall, and currently there is scarce information about the elicitor effect of its fragments in the fruit defense mechanism. In this work, the effect of the fragments of RGI on the defense mechanism in tomato (Solanum lycopersicum L.) fruits was evaluated. Three fragments of RGI released from enzymatic degradation by RG lyase were characterized according to their physical-chemical and structural characteristics. Two of RGI fragments (F-1 and F-2) were selected and applied on tomato fruits during 48 h. At different exposure times (0, 0.5, 1, 6, 24, and 48 h) tomato samples were taken, and the enzymatic activity of chitinase was assessed by fluorometry, and β-1,3-glucanase and peroxidase by spectrophotometry (UV-visible). The absorption bands determined by Fourier transform infra-red (FT-IR) spectroscopy in the RGI fragments, was different concerning RGI no fragmented. The RGI had a molecular weight of 64 kDa and an intrinsic viscosity of 40.9 mL g-1; while the molecular weight of F-1 and F-2 fragments were 20 and 16 kDa, and an average intrinsic viscosity of 27 and 26 mL g-1, respectively. The content of galacturonic acid decreased, and the neutral sugars increased concerning the time of enzymatic degradation. F-1 and F-2 fragments induced an increase in the level of β -1,3-glucanase activity in tomato fruits; however, an increase in the enzymatic activity of chitinase and peroxidase was observed after 0.5 h of exposure to F-2 fragment. It was concluded that the RGI fragments were structurally different from the RGI no fragmented and induced the natural defense mechanism of the tomato fruit.
author	Jiménez-Maldonado,María Isabel Tiznado-Hernández,Martín Ernesto Rascón-Chu,Agustín Carvajal-Millán,Elizabeth Lizardi-Mendoza,Jaime Troncoso-Rojas,Rosalba
author_facet	Jiménez-Maldonado,María Isabel Tiznado-Hernández,Martín Ernesto Rascón-Chu,Agustín Carvajal-Millán,Elizabeth Lizardi-Mendoza,Jaime Troncoso-Rojas,Rosalba
author_sort	Jiménez-Maldonado,María Isabel
title	Analysis of rhamnogalacturonan I fragments as elicitors of the defense mechanism in tomato fruit
title_short	Analysis of rhamnogalacturonan I fragments as elicitors of the defense mechanism in tomato fruit
title_full	Analysis of rhamnogalacturonan I fragments as elicitors of the defense mechanism in tomato fruit
title_fullStr	Analysis of rhamnogalacturonan I fragments as elicitors of the defense mechanism in tomato fruit
title_full_unstemmed	Analysis of rhamnogalacturonan I fragments as elicitors of the defense mechanism in tomato fruit
title_sort	analysis of rhamnogalacturonan i fragments as elicitors of the defense mechanism in tomato fruit
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392018000300339
work_keys_str_mv	AT jimenezmaldonadomariaisabel analysisofrhamnogalacturonanifragmentsaselicitorsofthedefensemechanismintomatofruit AT tiznadohernandezmartinernesto analysisofrhamnogalacturonanifragmentsaselicitorsofthedefensemechanismintomatofruit AT rasconchuagustin analysisofrhamnogalacturonanifragmentsaselicitorsofthedefensemechanismintomatofruit AT carvajalmillanelizabeth analysisofrhamnogalacturonanifragmentsaselicitorsofthedefensemechanismintomatofruit AT lizardimendozajaime analysisofrhamnogalacturonanifragmentsaselicitorsofthedefensemechanismintomatofruit AT troncosorojasrosalba analysisofrhamnogalacturonanifragmentsaselicitorsofthedefensemechanismintomatofruit
_version_	1714205379686039552

Analysis of rhamnogalacturonan I fragments as elicitors of the defense mechanism in tomato fruit

Ejemplares similares