Effect of saponins of Quillaja saponaria extracts in combination with Pseudomonas protegens to control Gaeumannomyces graminis var. tritici in wheat

ABSTRACT Extracts rich in saponins from Quillaja saponaria Mol. and populations of rhizobacteria from the genus Pseudomonas, which produce antimicrobial compounds, have been associated with reduction of the fungus Gaeumannomyces graminis var. tritici, the causal agent of take-all disease, which is r...

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Autores principales:	González-Castillo,Jonathan A., Quezada-D'Angelo,Tamara P., Silva-Aguayo,Gonzalo I., Moya-Elizondo,Ernesto A.
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2018
Materias:	Biofungicide 2,4-DAPG Pseudomonas protegens Quillaja saponaria saponins Triticum aestivum
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392018000300378
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spelling	oai:scielo:S0718-583920180003003782018-11-14Effect of saponins of Quillaja saponaria extracts in combination with Pseudomonas protegens to control Gaeumannomyces graminis var. tritici in wheatGonzález-Castillo,Jonathan A.Quezada-D'Angelo,Tamara P.Silva-Aguayo,Gonzalo I.Moya-Elizondo,Ernesto A. Biofungicide 2,4-DAPG Pseudomonas protegens Quillaja saponaria saponins Triticum aestivum ABSTRACT Extracts rich in saponins from Quillaja saponaria Mol. and populations of rhizobacteria from the genus Pseudomonas, which produce antimicrobial compounds, have been associated with reduction of the fungus Gaeumannomyces graminis var. tritici, the causal agent of take-all disease, which is responsible for severe loss of wheat (Triticum aestivum L.) crops worldwide. However, there is a limited background on the interaction between these bacteria and natural triterpenoids. The aim of this research was to assess the effect of saponin rich extract on Pseudomonas protegens strains 2,4-diacetylphloroglucinol-producers under in vitro and in plant conditions and determining the synergistic effect to be used together to control G. graminis var. tritici in wheat plants. We determined that 8% and 90% of saponins rich Q. saponaria extracts have a differential effect on P. protegens according to their purity (P ( 0.05). On wheat seedlings, quillaja extract with 90% of saponins did not affect the three antagonistic bacterial strain populations assessed, but affected biofilm formation at saponins concentration of 7360 mg L-1. Pseudomonas protegens strains had a variable antagonist activity in wheat plants, and controlled the fungus when were combined with different concentrations of pure Q. saponaria extract, with the concentration of 1840 ppm reducing the take-all disease in 32.5% with respect to the control inoculated with G. graminis var. tritici (P ( 0.05). However, no synergistic effects when the plant extract was combined with the bacterial strains were observed. These results showed the promising and complexity of combining bacterial and plant extract to develop a biopesticide, which could control this fungal disease.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.78 n.3 20182018-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392018000300378en10.4067/S0718-58392018000300378
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Biofungicide 2,4-DAPG Pseudomonas protegens Quillaja saponaria saponins Triticum aestivum
spellingShingle	Biofungicide 2,4-DAPG Pseudomonas protegens Quillaja saponaria saponins Triticum aestivum González-Castillo,Jonathan A. Quezada-D'Angelo,Tamara P. Silva-Aguayo,Gonzalo I. Moya-Elizondo,Ernesto A. Effect of saponins of Quillaja saponaria extracts in combination with Pseudomonas protegens to control Gaeumannomyces graminis var. tritici in wheat
description	ABSTRACT Extracts rich in saponins from Quillaja saponaria Mol. and populations of rhizobacteria from the genus Pseudomonas, which produce antimicrobial compounds, have been associated with reduction of the fungus Gaeumannomyces graminis var. tritici, the causal agent of take-all disease, which is responsible for severe loss of wheat (Triticum aestivum L.) crops worldwide. However, there is a limited background on the interaction between these bacteria and natural triterpenoids. The aim of this research was to assess the effect of saponin rich extract on Pseudomonas protegens strains 2,4-diacetylphloroglucinol-producers under in vitro and in plant conditions and determining the synergistic effect to be used together to control G. graminis var. tritici in wheat plants. We determined that 8% and 90% of saponins rich Q. saponaria extracts have a differential effect on P. protegens according to their purity (P ( 0.05). On wheat seedlings, quillaja extract with 90% of saponins did not affect the three antagonistic bacterial strain populations assessed, but affected biofilm formation at saponins concentration of 7360 mg L-1. Pseudomonas protegens strains had a variable antagonist activity in wheat plants, and controlled the fungus when were combined with different concentrations of pure Q. saponaria extract, with the concentration of 1840 ppm reducing the take-all disease in 32.5% with respect to the control inoculated with G. graminis var. tritici (P ( 0.05). However, no synergistic effects when the plant extract was combined with the bacterial strains were observed. These results showed the promising and complexity of combining bacterial and plant extract to develop a biopesticide, which could control this fungal disease.
author	González-Castillo,Jonathan A. Quezada-D'Angelo,Tamara P. Silva-Aguayo,Gonzalo I. Moya-Elizondo,Ernesto A.
author_facet	González-Castillo,Jonathan A. Quezada-D'Angelo,Tamara P. Silva-Aguayo,Gonzalo I. Moya-Elizondo,Ernesto A.
author_sort	González-Castillo,Jonathan A.
title	Effect of saponins of Quillaja saponaria extracts in combination with Pseudomonas protegens to control Gaeumannomyces graminis var. tritici in wheat
title_short	Effect of saponins of Quillaja saponaria extracts in combination with Pseudomonas protegens to control Gaeumannomyces graminis var. tritici in wheat
title_full	Effect of saponins of Quillaja saponaria extracts in combination with Pseudomonas protegens to control Gaeumannomyces graminis var. tritici in wheat
title_fullStr	Effect of saponins of Quillaja saponaria extracts in combination with Pseudomonas protegens to control Gaeumannomyces graminis var. tritici in wheat
title_full_unstemmed	Effect of saponins of Quillaja saponaria extracts in combination with Pseudomonas protegens to control Gaeumannomyces graminis var. tritici in wheat
title_sort	effect of saponins of quillaja saponaria extracts in combination with pseudomonas protegens to control gaeumannomyces graminis var. tritici in wheat
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392018000300378
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Effect of saponins of Quillaja saponaria extracts in combination with Pseudomonas protegens to control Gaeumannomyces graminis var. tritici in wheat

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