Slow growth in vitro culture for conservation of Chilotanum potato germplasm

ABSTRACT To keep potato (Solanum tuberosum L.) germplasm accessions disease-free and available for use, these are conserved as in vitro microplants under tissue culture conditions. The management of in vitro plants is labor intensive due to the necessity of periodic transferring of explants to new c...

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Autores principales:	Muñoz,Manuel, Díaz,Oscar, Reinún,Waleska, Winkler,Annelore, Quevedo,Roberto
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2019
Materias:	Growth modelling mannitol potato germplasm Solanum tuberosum sorbitol tissue culture.
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392019000100026
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spelling	oai:scielo:S0718-583920190001000262019-02-19Slow growth in vitro culture for conservation of Chilotanum potato germplasmMuñoz,ManuelDíaz,OscarReinún,WaleskaWinkler,AnneloreQuevedo,Roberto Growth modelling mannitol potato germplasm Solanum tuberosum sorbitol tissue culture. ABSTRACT To keep potato (Solanum tuberosum L.) germplasm accessions disease-free and available for use, these are conserved as in vitro microplants under tissue culture conditions. The management of in vitro plants is labor intensive due to the necessity of periodic transferring of explants to new containers and fresh medium (sub culturing). The effectiveness of MS medium supplemented with sorbitol or mannitol in conservation of potato germplasm corresponding to different genotypes from Chilean native landraces (Chilotanum group) was investigated. Growth curves for modelling, describing and predicting shoot elongation of in vitro potato plants through the time in different culture media were developed as a tool to plan subculture labor for refreshing explants to new media. In MS medium without osmotic active compounds the rate of shoot elongation (k) was 1.12-1.45 cm wk-1 with 0% mortality. In media supplemented with 20, 40, and 60 g L-1 sorbitol, k value ranged between 0.58-0.35, 0.42-0.27 and 0.09-0.05 cm wk-1 respectively. Mortality was 0%, 13%, and 26% for such treatments. In case of mannitol, k value ranged between 0.14-0.25, 0.065-0.11 and 0.042-0.068 cm wk with 3%, 6%, and 26% mortality for 20, 40, and 60 g L-1, respectively. These data can be used to predict shoot elongation rate in different media that provide several alternatives of speed of growth. The information allows to design an adequate strategy for organizing the work in a potato germplasm bank of S. tuberosum, Chilotanum group.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.79 n.1 20192019-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392019000100026en10.4067/S0718-58392019000100026
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Growth modelling mannitol potato germplasm Solanum tuberosum sorbitol tissue culture.
spellingShingle	Growth modelling mannitol potato germplasm Solanum tuberosum sorbitol tissue culture. Muñoz,Manuel Díaz,Oscar Reinún,Waleska Winkler,Annelore Quevedo,Roberto Slow growth in vitro culture for conservation of Chilotanum potato germplasm
description	ABSTRACT To keep potato (Solanum tuberosum L.) germplasm accessions disease-free and available for use, these are conserved as in vitro microplants under tissue culture conditions. The management of in vitro plants is labor intensive due to the necessity of periodic transferring of explants to new containers and fresh medium (sub culturing). The effectiveness of MS medium supplemented with sorbitol or mannitol in conservation of potato germplasm corresponding to different genotypes from Chilean native landraces (Chilotanum group) was investigated. Growth curves for modelling, describing and predicting shoot elongation of in vitro potato plants through the time in different culture media were developed as a tool to plan subculture labor for refreshing explants to new media. In MS medium without osmotic active compounds the rate of shoot elongation (k) was 1.12-1.45 cm wk-1 with 0% mortality. In media supplemented with 20, 40, and 60 g L-1 sorbitol, k value ranged between 0.58-0.35, 0.42-0.27 and 0.09-0.05 cm wk-1 respectively. Mortality was 0%, 13%, and 26% for such treatments. In case of mannitol, k value ranged between 0.14-0.25, 0.065-0.11 and 0.042-0.068 cm wk with 3%, 6%, and 26% mortality for 20, 40, and 60 g L-1, respectively. These data can be used to predict shoot elongation rate in different media that provide several alternatives of speed of growth. The information allows to design an adequate strategy for organizing the work in a potato germplasm bank of S. tuberosum, Chilotanum group.
author	Muñoz,Manuel Díaz,Oscar Reinún,Waleska Winkler,Annelore Quevedo,Roberto
author_facet	Muñoz,Manuel Díaz,Oscar Reinún,Waleska Winkler,Annelore Quevedo,Roberto
author_sort	Muñoz,Manuel
title	Slow growth in vitro culture for conservation of Chilotanum potato germplasm
title_short	Slow growth in vitro culture for conservation of Chilotanum potato germplasm
title_full	Slow growth in vitro culture for conservation of Chilotanum potato germplasm
title_fullStr	Slow growth in vitro culture for conservation of Chilotanum potato germplasm
title_full_unstemmed	Slow growth in vitro culture for conservation of Chilotanum potato germplasm
title_sort	slow growth in vitro culture for conservation of chilotanum potato germplasm
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392019000100026
work_keys_str_mv	AT munozmanuel slowgrowthinvitrocultureforconservationofchilotanumpotatogermplasm AT diazoscar slowgrowthinvitrocultureforconservationofchilotanumpotatogermplasm AT reinunwaleska slowgrowthinvitrocultureforconservationofchilotanumpotatogermplasm AT winklerannelore slowgrowthinvitrocultureforconservationofchilotanumpotatogermplasm AT quevedoroberto slowgrowthinvitrocultureforconservationofchilotanumpotatogermplasm
_version_	1714205384296628224

Slow growth in vitro culture for conservation of Chilotanum potato germplasm

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