Transcriptome analysis of hot pepper plants identifies waterlogging resistance related genes

ABSTRACT Hot pepper (Capsicum annuum L.) is one of the most important vegetable crops in China, but floods bring substantial decreases in production over the past several decades. In order to investigate the mechanisms of waterlogging resistance in mutant hot pepper, we measured the agronomic traits...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhang,Yuping, Ou,Lijun, Zhao,Ji, Liu,Zhoubin, Li,Xuefeng
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2019
Materias:	Capsicum annuum gene hot pepper mutant transcriptomic data waterlogging.
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392019000200296
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-58392019000200296
record_format	dspace
spelling	oai:scielo:S0718-583920190002002962019-05-14Transcriptome analysis of hot pepper plants identifies waterlogging resistance related genesZhang,YupingOu,LijunZhao,JiLiu,ZhoubinLi,Xuefeng Capsicum annuum gene hot pepper mutant transcriptomic data waterlogging. ABSTRACT Hot pepper (Capsicum annuum L.) is one of the most important vegetable crops in China, but floods bring substantial decreases in production over the past several decades. In order to investigate the mechanisms of waterlogging resistance in mutant hot pepper, we measured the agronomic traits of mutant and wild-type, as well as the activities of root antioxidant enzymes and the contents of osmotic regulation substance. At the same time, we did transcriptome sequencing on the plant roots, and screened for differentially expressed genes between mutant and wild-type. The results showed that, under waterlogging stress, the mutants could grow normally, and the activities of their superoxide dismutase, peroxidase, catalase, and glutathione reductase were significantly increased, as well as the contents of proline and soluble sugar. The accumulation of malondialdehyde and hydroxyl radical in mutants was significantly reduced. Among the 61 differentially expressed genes from transcriptome analysis, 24 genes were up-regulated and 37 genes were down-regulated in mutants. After functional analysis, we found 8 genes related to the metabolism of endogenous hormone and protective enzymes, among which, auxin-induced protein related gene cap.ARATH, ethylene response related gene Cap.RAP2, MYB family related gene Cap.MYB1R1, and the 4 genes related to peroxidase Cap.POD, were significantly up-regulated in mutants, while the Capana01g001329 gene was down-regulated. These results suggest that under waterlogging stress, the mutant could enhance its resistance to waterlogging by regulating the genes involved in metabolism of endogenous hormones and protective enzymes. This study provides the scientific basis for elucidating the gene regulation network of mutant pepper under waterlogging stressinfo:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.79 n.2 20192019-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392019000200296en10.4067/S0718-58392019000200296
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Capsicum annuum gene hot pepper mutant transcriptomic data waterlogging.
spellingShingle	Capsicum annuum gene hot pepper mutant transcriptomic data waterlogging. Zhang,Yuping Ou,Lijun Zhao,Ji Liu,Zhoubin Li,Xuefeng Transcriptome analysis of hot pepper plants identifies waterlogging resistance related genes
description	ABSTRACT Hot pepper (Capsicum annuum L.) is one of the most important vegetable crops in China, but floods bring substantial decreases in production over the past several decades. In order to investigate the mechanisms of waterlogging resistance in mutant hot pepper, we measured the agronomic traits of mutant and wild-type, as well as the activities of root antioxidant enzymes and the contents of osmotic regulation substance. At the same time, we did transcriptome sequencing on the plant roots, and screened for differentially expressed genes between mutant and wild-type. The results showed that, under waterlogging stress, the mutants could grow normally, and the activities of their superoxide dismutase, peroxidase, catalase, and glutathione reductase were significantly increased, as well as the contents of proline and soluble sugar. The accumulation of malondialdehyde and hydroxyl radical in mutants was significantly reduced. Among the 61 differentially expressed genes from transcriptome analysis, 24 genes were up-regulated and 37 genes were down-regulated in mutants. After functional analysis, we found 8 genes related to the metabolism of endogenous hormone and protective enzymes, among which, auxin-induced protein related gene cap.ARATH, ethylene response related gene Cap.RAP2, MYB family related gene Cap.MYB1R1, and the 4 genes related to peroxidase Cap.POD, were significantly up-regulated in mutants, while the Capana01g001329 gene was down-regulated. These results suggest that under waterlogging stress, the mutant could enhance its resistance to waterlogging by regulating the genes involved in metabolism of endogenous hormones and protective enzymes. This study provides the scientific basis for elucidating the gene regulation network of mutant pepper under waterlogging stress
author	Zhang,Yuping Ou,Lijun Zhao,Ji Liu,Zhoubin Li,Xuefeng
author_facet	Zhang,Yuping Ou,Lijun Zhao,Ji Liu,Zhoubin Li,Xuefeng
author_sort	Zhang,Yuping
title	Transcriptome analysis of hot pepper plants identifies waterlogging resistance related genes
title_short	Transcriptome analysis of hot pepper plants identifies waterlogging resistance related genes
title_full	Transcriptome analysis of hot pepper plants identifies waterlogging resistance related genes
title_fullStr	Transcriptome analysis of hot pepper plants identifies waterlogging resistance related genes
title_full_unstemmed	Transcriptome analysis of hot pepper plants identifies waterlogging resistance related genes
title_sort	transcriptome analysis of hot pepper plants identifies waterlogging resistance related genes
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392019000200296
work_keys_str_mv	AT zhangyuping transcriptomeanalysisofhotpepperplantsidentifieswaterloggingresistancerelatedgenes AT oulijun transcriptomeanalysisofhotpepperplantsidentifieswaterloggingresistancerelatedgenes AT zhaoji transcriptomeanalysisofhotpepperplantsidentifieswaterloggingresistancerelatedgenes AT liuzhoubin transcriptomeanalysisofhotpepperplantsidentifieswaterloggingresistancerelatedgenes AT lixuefeng transcriptomeanalysisofhotpepperplantsidentifieswaterloggingresistancerelatedgenes
_version_	1714205388785582080

Transcriptome analysis of hot pepper plants identifies waterlogging resistance related genes

Ejemplares similares