PCR diagnosis and in vitro sanitation of the papaya MSXJ hybrid with ringspot virus disease

ABSTRACT Papaya (Carica papaya L.) is a tropical fruit with a high nutritional value. Current problems affecting its cultivation are the limited number of exploited varieties and its susceptibility to pests and diseases. The papaya MSXJ hybrid is resistant to pests and diseases, and its organoleptic...

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Autores principales:	González-Lázaro,Mariel, Hernández-Rosas,Francisco, Gómez-Merino,Fernando Carlos, Pastelín-Solano,Miriam Cristina, Bulbarela-Marini,Javier Emanuel, Solano-Rodríguez,Luis Alberto, Castro,Odón Castañeda
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2019
Materias:	Apical meristems Carica papaya Papaya ringspot virus (PRSV) RT-PCR thermotherapy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392019000300376
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spelling	oai:scielo:S0718-583920190003003762019-07-18PCR diagnosis and in vitro sanitation of the papaya MSXJ hybrid with ringspot virus diseaseGonzález-Lázaro,MarielHernández-Rosas,FranciscoGómez-Merino,Fernando CarlosPastelín-Solano,Miriam CristinaBulbarela-Marini,Javier EmanuelSolano-Rodríguez,Luis AlbertoCastro,Odón Castañeda Apical meristems Carica papaya Papaya ringspot virus (PRSV) RT-PCR thermotherapy ABSTRACT Papaya (Carica papaya L.) is a tropical fruit with a high nutritional value. Current problems affecting its cultivation are the limited number of exploited varieties and its susceptibility to pests and diseases. The papaya MSXJ hybrid is resistant to pests and diseases, and its organoleptic characteristics are prominent compared with most papaya genotypes on the market. Samples of Papaya ringspot virus (PRSV) disease were evaluated by in vitro culture of the apical meristems and thermotherapy. Sanitation of apical meristems was accomplished by regenerating them with or without light and thermotherapy was performed at 40 and 45 °C for 4 and 7 h, respectively. Both situations were observed for 45 d to evaluate survival, regeneration, and sanitation by reverse transcription polymerase chain reaction (RT-PCR). In vitro culture of the meristems in total darkness obtained the highest survival rate (87%) and regeneration (22%) compared with the treatments under photoperiod (21% and 18%, respectively). Under both study conditions, sanitation in PRSV was 100% of the meristems. Plants subjected to thermotherapy showed a higher survival rate (99.92%) at 40 °C for 4 h and sanitation of 80.2%info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.79 n.3 20192019-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392019000300376en10.4067/S0718-58392019000300376
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Apical meristems Carica papaya Papaya ringspot virus (PRSV) RT-PCR thermotherapy
spellingShingle	Apical meristems Carica papaya Papaya ringspot virus (PRSV) RT-PCR thermotherapy González-Lázaro,Mariel Hernández-Rosas,Francisco Gómez-Merino,Fernando Carlos Pastelín-Solano,Miriam Cristina Bulbarela-Marini,Javier Emanuel Solano-Rodríguez,Luis Alberto Castro,Odón Castañeda PCR diagnosis and in vitro sanitation of the papaya MSXJ hybrid with ringspot virus disease
description	ABSTRACT Papaya (Carica papaya L.) is a tropical fruit with a high nutritional value. Current problems affecting its cultivation are the limited number of exploited varieties and its susceptibility to pests and diseases. The papaya MSXJ hybrid is resistant to pests and diseases, and its organoleptic characteristics are prominent compared with most papaya genotypes on the market. Samples of Papaya ringspot virus (PRSV) disease were evaluated by in vitro culture of the apical meristems and thermotherapy. Sanitation of apical meristems was accomplished by regenerating them with or without light and thermotherapy was performed at 40 and 45 °C for 4 and 7 h, respectively. Both situations were observed for 45 d to evaluate survival, regeneration, and sanitation by reverse transcription polymerase chain reaction (RT-PCR). In vitro culture of the meristems in total darkness obtained the highest survival rate (87%) and regeneration (22%) compared with the treatments under photoperiod (21% and 18%, respectively). Under both study conditions, sanitation in PRSV was 100% of the meristems. Plants subjected to thermotherapy showed a higher survival rate (99.92%) at 40 °C for 4 h and sanitation of 80.2%
author	González-Lázaro,Mariel Hernández-Rosas,Francisco Gómez-Merino,Fernando Carlos Pastelín-Solano,Miriam Cristina Bulbarela-Marini,Javier Emanuel Solano-Rodríguez,Luis Alberto Castro,Odón Castañeda
author_facet	González-Lázaro,Mariel Hernández-Rosas,Francisco Gómez-Merino,Fernando Carlos Pastelín-Solano,Miriam Cristina Bulbarela-Marini,Javier Emanuel Solano-Rodríguez,Luis Alberto Castro,Odón Castañeda
author_sort	González-Lázaro,Mariel
title	PCR diagnosis and in vitro sanitation of the papaya MSXJ hybrid with ringspot virus disease
title_short	PCR diagnosis and in vitro sanitation of the papaya MSXJ hybrid with ringspot virus disease
title_full	PCR diagnosis and in vitro sanitation of the papaya MSXJ hybrid with ringspot virus disease
title_fullStr	PCR diagnosis and in vitro sanitation of the papaya MSXJ hybrid with ringspot virus disease
title_full_unstemmed	PCR diagnosis and in vitro sanitation of the papaya MSXJ hybrid with ringspot virus disease
title_sort	pcr diagnosis and in vitro sanitation of the papaya msxj hybrid with ringspot virus disease
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392019000300376
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PCR diagnosis and in vitro sanitation of the papaya MSXJ hybrid with ringspot virus disease

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