Identification of a phytoplasma associated with witches’ broom symptoms in calafate (Berberis microphylla G. Forst.)

Identification of a phytoplasma associated with witches’ broom symptoms in calafate (Berberis microphylla G. Forst.)

ABSTRACT Berberis microphylla G. Forst. (“calafate”) is a native berry grown in the Patagonian area of Chile and Argentina. The calafate fruit have important commercial projection, given that contains antioxidant characteristics with high levels of phenolic compounds and anthocya...

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Autores principales: Madariaga,Mónica, Ramírez,Isabel
Lenguaje:English
Publicado: Instituto de Investigaciones Agropecuarias, INIA 2019
Materias:
Calafate
phytoplasmas
witch’s broom
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392019000300493
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spelling oai:scielo:S0718-583920190003004932019-07-18Identification of a phytoplasma associated with witches’ broom symptoms in calafate (Berberis microphylla G. Forst.)Madariaga,MónicaRamírez,Isabel Calafate phytoplasmas witch’s broom ABSTRACT Berberis microphylla G. Forst. (“calafate”) is a native berry grown in the Patagonian area of Chile and Argentina. The calafate fruit have important commercial projection, given that contains antioxidant characteristics with high levels of phenolic compounds and anthocyanins. Recently, wild growth of calafate present in Region of Magallanes y la Antártica Chilena has been affected by symptoms of witch’s broom, which is associated to infections caused by phytoplasmas. To determine the presence of phytoplasmas in calafate plants affected by these symptoms, symptomatic and asymptomatic samples were analyzed. To identify the phytopathogen, nested polymerase chain reaction (nested-PCR) was used with three pairs of primers (P1/P7, R16mF2/mR1, R16F2n/R2); sequencing of the fragments obtained from PCR and an in silico restriction analysis was carried out on the obtained genomic sequences. Results from nested-PCR showed an expected band of 1.2 kb only in the samples with symptoms. The nucleotide sequence obtained from the amplified DNA fragment had similarity to 99% of other phytoplasmas present in the National Center for Biotechnology Information (NCBI) database, according to a basic local alignment search tool (BLAST) analysis. Restriction analysis indicated that the amplified DNA fragment corresponded to a phytoplasma from the 16SrXIII-f group. In conclusion, the symptoms of witch’s broom present in the analyzed calafate plants from Magallanes region signal the presence of the phytoplasma “Candidatus Phytoplasma hispanicum” pertaining to the group 16SrXIII-Finfo:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.79 n.3 20192019-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392019000300493en10.4067/S0718-58392019000300493
institution Scielo Chile
collection Scielo Chile
language English
topic Calafate
phytoplasmas
witch’s broom
spellingShingle Calafate
phytoplasmas
witch’s broom
Madariaga,Mónica
Ramírez,Isabel
Identification of a phytoplasma associated with witches’ broom symptoms in calafate (Berberis microphylla G. Forst.)
description ABSTRACT Berberis microphylla G. Forst. (“calafate”) is a native berry grown in the Patagonian area of Chile and Argentina. The calafate fruit have important commercial projection, given that contains antioxidant characteristics with high levels of phenolic compounds and anthocyanins. Recently, wild growth of calafate present in Region of Magallanes y la Antártica Chilena has been affected by symptoms of witch’s broom, which is associated to infections caused by phytoplasmas. To determine the presence of phytoplasmas in calafate plants affected by these symptoms, symptomatic and asymptomatic samples were analyzed. To identify the phytopathogen, nested polymerase chain reaction (nested-PCR) was used with three pairs of primers (P1/P7, R16mF2/mR1, R16F2n/R2); sequencing of the fragments obtained from PCR and an in silico restriction analysis was carried out on the obtained genomic sequences. Results from nested-PCR showed an expected band of 1.2 kb only in the samples with symptoms. The nucleotide sequence obtained from the amplified DNA fragment had similarity to 99% of other phytoplasmas present in the National Center for Biotechnology Information (NCBI) database, according to a basic local alignment search tool (BLAST) analysis. Restriction analysis indicated that the amplified DNA fragment corresponded to a phytoplasma from the 16SrXIII-f group. In conclusion, the symptoms of witch’s broom present in the analyzed calafate plants from Magallanes region signal the presence of the phytoplasma “Candidatus Phytoplasma hispanicum” pertaining to the group 16SrXIII-F
author Madariaga,Mónica
Ramírez,Isabel
author_facet Madariaga,Mónica
Ramírez,Isabel
author_sort Madariaga,Mónica
title Identification of a phytoplasma associated with witches’ broom symptoms in calafate (Berberis microphylla G. Forst.)
title_short Identification of a phytoplasma associated with witches’ broom symptoms in calafate (Berberis microphylla G. Forst.)
title_full Identification of a phytoplasma associated with witches’ broom symptoms in calafate (Berberis microphylla G. Forst.)
title_fullStr Identification of a phytoplasma associated with witches’ broom symptoms in calafate (Berberis microphylla G. Forst.)
title_full_unstemmed Identification of a phytoplasma associated with witches’ broom symptoms in calafate (Berberis microphylla G. Forst.)
title_sort identification of a phytoplasma associated with witches’ broom symptoms in calafate (berberis microphylla g. forst.)
publisher Instituto de Investigaciones Agropecuarias, INIA
publishDate 2019
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392019000300493
work_keys_str_mv AT madariagamonica identificationofaphytoplasmaassociatedwithwitches8217broomsymptomsincalafateberberismicrophyllagforst
AT ramirezisabel identificationofaphytoplasmaassociatedwithwitches8217broomsymptomsincalafateberberismicrophyllagforst
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