Optimization of media formulation for axillary shoot multiplication of the red-peeled sweet potato (Ipomoea batatas [L.] Lam.) ‘Abees’

ABSTRACT Sweet potato (Ipomoea batatas [L.] Lam.) is an important food crop that is cultivated and consumed worldwide, and its nutritional value makes it especially important in developing countries. However, sweet potato plants produced using conventional vegetative propagation can be prone to seve...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dewir,Yaser Hassan, Aldubai,Abdulhakim A., Kher,Mafatlal M., Alsadon,Abdullah A., El-Hendawy,Salah, Al-Suhaibani,Nasser A.
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2020
Materias:	Convolvulaceae culture duration micropropagation oxalic acid phenylacetic acid silver nitrate.
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000100003
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-58392020000100003
record_format	dspace
spelling	oai:scielo:S0718-583920200001000032020-02-25Optimization of media formulation for axillary shoot multiplication of the red-peeled sweet potato (Ipomoea batatas [L.] Lam.) ‘Abees’Dewir,Yaser HassanAldubai,Abdulhakim A.Kher,Mafatlal M.Alsadon,Abdullah A.El-Hendawy,SalahAl-Suhaibani,Nasser A. Convolvulaceae culture duration micropropagation oxalic acid phenylacetic acid silver nitrate. ABSTRACT Sweet potato (Ipomoea batatas [L.] Lam.) is an important food crop that is cultivated and consumed worldwide, and its nutritional value makes it especially important in developing countries. However, sweet potato plants produced using conventional vegetative propagation can be prone to several diseases, thereby hindering crop yield. Therefore, micropropagation techniques are useful for producing higher quality plant material. The aim of the present study was to determine the optimal medium formulation for the axillary shoot multiplication of single-node ‘Abees’ explants. The influences of cytokinins (6-benzyladenine [BA], kinetin, thidiazuron, and zeatin), gibberellic acid, phenylacetic acid, indole-3-butyric acid, silver nitrate, and oxalic acid, culture duration, and medium salt strength were investigated. Optimal proliferation was observed when shoots were cultured on half-strength MS medium that was supplemented with 2 mg L-1 BA and 100 mg L-1 oxalic acid for 2 wk, followed by culture on half-strength MS medium without plant growth regulators for 4 wk. In this medium, the greatest number of shoots (3.1) and total number of nodes (16.1) per explant were observed. All (100%) of the regenerated shoots were rooted and acclimatized, with a survival rate of 96% when transferred to ex vitro conditions and no morphological abnormalities.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.80 n.1 20202020-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000100003en10.4067/S0718-58392020000100003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Convolvulaceae culture duration micropropagation oxalic acid phenylacetic acid silver nitrate.
spellingShingle	Convolvulaceae culture duration micropropagation oxalic acid phenylacetic acid silver nitrate. Dewir,Yaser Hassan Aldubai,Abdulhakim A. Kher,Mafatlal M. Alsadon,Abdullah A. El-Hendawy,Salah Al-Suhaibani,Nasser A. Optimization of media formulation for axillary shoot multiplication of the red-peeled sweet potato (Ipomoea batatas [L.] Lam.) ‘Abees’
description	ABSTRACT Sweet potato (Ipomoea batatas [L.] Lam.) is an important food crop that is cultivated and consumed worldwide, and its nutritional value makes it especially important in developing countries. However, sweet potato plants produced using conventional vegetative propagation can be prone to several diseases, thereby hindering crop yield. Therefore, micropropagation techniques are useful for producing higher quality plant material. The aim of the present study was to determine the optimal medium formulation for the axillary shoot multiplication of single-node ‘Abees’ explants. The influences of cytokinins (6-benzyladenine [BA], kinetin, thidiazuron, and zeatin), gibberellic acid, phenylacetic acid, indole-3-butyric acid, silver nitrate, and oxalic acid, culture duration, and medium salt strength were investigated. Optimal proliferation was observed when shoots were cultured on half-strength MS medium that was supplemented with 2 mg L-1 BA and 100 mg L-1 oxalic acid for 2 wk, followed by culture on half-strength MS medium without plant growth regulators for 4 wk. In this medium, the greatest number of shoots (3.1) and total number of nodes (16.1) per explant were observed. All (100%) of the regenerated shoots were rooted and acclimatized, with a survival rate of 96% when transferred to ex vitro conditions and no morphological abnormalities.
author	Dewir,Yaser Hassan Aldubai,Abdulhakim A. Kher,Mafatlal M. Alsadon,Abdullah A. El-Hendawy,Salah Al-Suhaibani,Nasser A.
author_facet	Dewir,Yaser Hassan Aldubai,Abdulhakim A. Kher,Mafatlal M. Alsadon,Abdullah A. El-Hendawy,Salah Al-Suhaibani,Nasser A.
author_sort	Dewir,Yaser Hassan
title	Optimization of media formulation for axillary shoot multiplication of the red-peeled sweet potato (Ipomoea batatas [L.] Lam.) ‘Abees’
title_short	Optimization of media formulation for axillary shoot multiplication of the red-peeled sweet potato (Ipomoea batatas [L.] Lam.) ‘Abees’
title_full	Optimization of media formulation for axillary shoot multiplication of the red-peeled sweet potato (Ipomoea batatas [L.] Lam.) ‘Abees’
title_fullStr	Optimization of media formulation for axillary shoot multiplication of the red-peeled sweet potato (Ipomoea batatas [L.] Lam.) ‘Abees’
title_full_unstemmed	Optimization of media formulation for axillary shoot multiplication of the red-peeled sweet potato (Ipomoea batatas [L.] Lam.) ‘Abees’
title_sort	optimization of media formulation for axillary shoot multiplication of the red-peeled sweet potato (ipomoea batatas [l.] lam.) ‘abees’
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000100003
work_keys_str_mv	AT dewiryaserhassan optimizationofmediaformulationforaxillaryshootmultiplicationoftheredpeeledsweetpotatoipomoeabatatasllam8216abees8217 AT aldubaiabdulhakima optimizationofmediaformulationforaxillaryshootmultiplicationoftheredpeeledsweetpotatoipomoeabatatasllam8216abees8217 AT khermafatlalm optimizationofmediaformulationforaxillaryshootmultiplicationoftheredpeeledsweetpotatoipomoeabatatasllam8216abees8217 AT alsadonabdullaha optimizationofmediaformulationforaxillaryshootmultiplicationoftheredpeeledsweetpotatoipomoeabatatasllam8216abees8217 AT elhendawysalah optimizationofmediaformulationforaxillaryshootmultiplicationoftheredpeeledsweetpotatoipomoeabatatasllam8216abees8217 AT alsuhaibaninassera optimizationofmediaformulationforaxillaryshootmultiplicationoftheredpeeledsweetpotatoipomoeabatatasllam8216abees8217
_version_	1714205395159875584

Optimization of media formulation for axillary shoot multiplication of the red-peeled sweet potato (Ipomoea batatas [L.] Lam.) &#8216;Abees&#8217;

Ejemplares similares

Optimization of media formulation for axillary shoot multiplication of the red-peeled sweet potato (Ipomoea batatas [L.] Lam.) ‘Abees’