New polymorphic nuclear microsatellites from Aristotelia chilensis (Mol.) Stuntz (Elaeocarpaceae)

ABSTRACT Maqui (Aristotelia chilensis [Molina] Stuntz) is a dioecious small tree native to Chile and southwestern Argentina. This species has gained attention due to its high polyphenol content and anti-oxidant capacity. Nevertheless, genetics studies and information about A. chilensis population ge...

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Autores principales:	Cona,Matías, Castro,Mª Herminia, León-Lobos,Pedro, Correa,Francisco, Jordan,Greg, Bastías,Adriana, Sagredo,Boris, Hinrichsen,Patricio
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2020
Materias:	Fingerprinting maqui maqui berry molecular markers Patagonian endemics SSR
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000200153
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spelling	oai:scielo:S0718-583920200002001532020-11-30New polymorphic nuclear microsatellites from Aristotelia chilensis (Mol.) Stuntz (Elaeocarpaceae)Cona,MatíasCastro,Mª HerminiaLeón-Lobos,PedroCorrea,FranciscoJordan,GregBastías,AdrianaSagredo,BorisHinrichsen,Patricio Fingerprinting maqui maqui berry molecular markers Patagonian endemics SSR ABSTRACT Maqui (Aristotelia chilensis [Molina] Stuntz) is a dioecious small tree native to Chile and southwestern Argentina. This species has gained attention due to its high polyphenol content and anti-oxidant capacity. Nevertheless, genetics studies and information about A. chilensis population genetics are scarce and even contradictory. In fact, the available species-specific simple sequence repeat (SSR) markers are not informative at all, and so we decided to identify and characterize new ones able to trace individual genotypes, a basic tool intended for different genetic studies. We identified and characterized 15 new polymorphic SSR markers for A. chilensis. These markers were evaluated in three populations distributed along 1000 km of Central Chile, exhibiting up to 10 alleles per locus and a combined expected heterozygosity of 0.858. Markers were also informative in two related species, Aristotelia peduncularis (Labill.) Hook. f. and Crinodendron patagua Molina (Elaeocarpaceae), with 13 and six SSRs showing clear amplification patterns, respectively. This new set of SSR markers are highly polymorphic and informative, being the first ones available for the effective fingerprinting of maqui genotypes. A proof of concept of that was the differentiation of six maqui accessions that are under domestication for productive purposes, based on a subset of the polymorphic SSR markers.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.80 n.2 20202020-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000200153en10.4067/S0718-58392020000200153
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Fingerprinting maqui maqui berry molecular markers Patagonian endemics SSR
spellingShingle	Fingerprinting maqui maqui berry molecular markers Patagonian endemics SSR Cona,Matías Castro,Mª Herminia León-Lobos,Pedro Correa,Francisco Jordan,Greg Bastías,Adriana Sagredo,Boris Hinrichsen,Patricio New polymorphic nuclear microsatellites from Aristotelia chilensis (Mol.) Stuntz (Elaeocarpaceae)
description	ABSTRACT Maqui (Aristotelia chilensis [Molina] Stuntz) is a dioecious small tree native to Chile and southwestern Argentina. This species has gained attention due to its high polyphenol content and anti-oxidant capacity. Nevertheless, genetics studies and information about A. chilensis population genetics are scarce and even contradictory. In fact, the available species-specific simple sequence repeat (SSR) markers are not informative at all, and so we decided to identify and characterize new ones able to trace individual genotypes, a basic tool intended for different genetic studies. We identified and characterized 15 new polymorphic SSR markers for A. chilensis. These markers were evaluated in three populations distributed along 1000 km of Central Chile, exhibiting up to 10 alleles per locus and a combined expected heterozygosity of 0.858. Markers were also informative in two related species, Aristotelia peduncularis (Labill.) Hook. f. and Crinodendron patagua Molina (Elaeocarpaceae), with 13 and six SSRs showing clear amplification patterns, respectively. This new set of SSR markers are highly polymorphic and informative, being the first ones available for the effective fingerprinting of maqui genotypes. A proof of concept of that was the differentiation of six maqui accessions that are under domestication for productive purposes, based on a subset of the polymorphic SSR markers.
author	Cona,Matías Castro,Mª Herminia León-Lobos,Pedro Correa,Francisco Jordan,Greg Bastías,Adriana Sagredo,Boris Hinrichsen,Patricio
author_facet	Cona,Matías Castro,Mª Herminia León-Lobos,Pedro Correa,Francisco Jordan,Greg Bastías,Adriana Sagredo,Boris Hinrichsen,Patricio
author_sort	Cona,Matías
title	New polymorphic nuclear microsatellites from Aristotelia chilensis (Mol.) Stuntz (Elaeocarpaceae)
title_short	New polymorphic nuclear microsatellites from Aristotelia chilensis (Mol.) Stuntz (Elaeocarpaceae)
title_full	New polymorphic nuclear microsatellites from Aristotelia chilensis (Mol.) Stuntz (Elaeocarpaceae)
title_fullStr	New polymorphic nuclear microsatellites from Aristotelia chilensis (Mol.) Stuntz (Elaeocarpaceae)
title_full_unstemmed	New polymorphic nuclear microsatellites from Aristotelia chilensis (Mol.) Stuntz (Elaeocarpaceae)
title_sort	new polymorphic nuclear microsatellites from aristotelia chilensis (mol.) stuntz (elaeocarpaceae)
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000200153
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New polymorphic nuclear microsatellites from Aristotelia chilensis (Mol.) Stuntz (Elaeocarpaceae)

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