Calcium chloride priming increases chilling tolerance in Salvia miltiorrhiza Bunge

ABSTRACT Chilling stress inhibits germination and early seedling growth of Salvia miltiorrhiza Bunge. The present study was carried out to investigate the effects of osmopriming with CaCl2 on the germination and early seedling growth of S. miltiorrhiza under chilling stress (10 ℃). Seeds w...

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Autores principales:	Gao,Lu, Yan,Min
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2020
Materias:	Calcium chloride chilling stress Salvia miltiorrhiza seed priming
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000200219
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spelling	oai:scielo:S0718-583920200002002192020-11-30Calcium chloride priming increases chilling tolerance in Salvia miltiorrhiza BungeGao,LuYan,Min Calcium chloride chilling stress Salvia miltiorrhiza seed priming ABSTRACT Chilling stress inhibits germination and early seedling growth of Salvia miltiorrhiza Bunge. The present study was carried out to investigate the effects of osmopriming with CaCl2 on the germination and early seedling growth of S. miltiorrhiza under chilling stress (10 ℃). Seeds were soaked in different concentrations of CaCl2 solutions (0, 50, 100, and 150 mM) at 20 ℃ for 24 h in darkness, rinsed, and air-dried. The effects of seed priming were evaluated on germination and survival during subsequent exposure to chilling stress (10 ℃) for 14 d. An unprimed control treatment was sown under optimal temperatures (25 ℃). Results indicated that germination percentage, germination potential, and seedling vigor index of unprimed seeds decreased from 69.2%, 12.4, and 0.092 to 28%, 3.6, and 0.022, respectively, when subjected to chilling stress. Higher malondialdehyde (MDA) content and lower chlorophyll contents (including total chlorophyll, chlorophyll a, and chlorophyll b) were detected in S. miltiorrhiza seedlings. Osmopriming with 100 and 150 mM CaCl2 effectively lessened the negative effects of chilling stress. Germination percentage, germination potential, and seedling vigor index of primed seeds reached 42% and 48%, 6.36 and 8.78, and 0.037 and 0.047, respectively. Meanwhile, CaCl2 priming significantly triggered superoxide dismutase, peroxidase, and catalase antioxidant enzyme activity, enhanced the accumulation of osmoregulation substances (soluble protein, accumulation sugars, and proline), increased the photosynthetic pigment content (total chlorophyll, chlorophyll a, chlorophyll b, and carotenoids), and decreased the MDA content. The most effective concentration of CaCl2 priming was 150 mM.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.80 n.2 20202020-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000200219en10.4067/S0718-58392020000200219
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Calcium chloride chilling stress Salvia miltiorrhiza seed priming
spellingShingle	Calcium chloride chilling stress Salvia miltiorrhiza seed priming Gao,Lu Yan,Min Calcium chloride priming increases chilling tolerance in Salvia miltiorrhiza Bunge
description	ABSTRACT Chilling stress inhibits germination and early seedling growth of Salvia miltiorrhiza Bunge. The present study was carried out to investigate the effects of osmopriming with CaCl2 on the germination and early seedling growth of S. miltiorrhiza under chilling stress (10 ℃). Seeds were soaked in different concentrations of CaCl2 solutions (0, 50, 100, and 150 mM) at 20 ℃ for 24 h in darkness, rinsed, and air-dried. The effects of seed priming were evaluated on germination and survival during subsequent exposure to chilling stress (10 ℃) for 14 d. An unprimed control treatment was sown under optimal temperatures (25 ℃). Results indicated that germination percentage, germination potential, and seedling vigor index of unprimed seeds decreased from 69.2%, 12.4, and 0.092 to 28%, 3.6, and 0.022, respectively, when subjected to chilling stress. Higher malondialdehyde (MDA) content and lower chlorophyll contents (including total chlorophyll, chlorophyll a, and chlorophyll b) were detected in S. miltiorrhiza seedlings. Osmopriming with 100 and 150 mM CaCl2 effectively lessened the negative effects of chilling stress. Germination percentage, germination potential, and seedling vigor index of primed seeds reached 42% and 48%, 6.36 and 8.78, and 0.037 and 0.047, respectively. Meanwhile, CaCl2 priming significantly triggered superoxide dismutase, peroxidase, and catalase antioxidant enzyme activity, enhanced the accumulation of osmoregulation substances (soluble protein, accumulation sugars, and proline), increased the photosynthetic pigment content (total chlorophyll, chlorophyll a, chlorophyll b, and carotenoids), and decreased the MDA content. The most effective concentration of CaCl2 priming was 150 mM.
author	Gao,Lu Yan,Min
author_facet	Gao,Lu Yan,Min
author_sort	Gao,Lu
title	Calcium chloride priming increases chilling tolerance in Salvia miltiorrhiza Bunge
title_short	Calcium chloride priming increases chilling tolerance in Salvia miltiorrhiza Bunge
title_full	Calcium chloride priming increases chilling tolerance in Salvia miltiorrhiza Bunge
title_fullStr	Calcium chloride priming increases chilling tolerance in Salvia miltiorrhiza Bunge
title_full_unstemmed	Calcium chloride priming increases chilling tolerance in Salvia miltiorrhiza Bunge
title_sort	calcium chloride priming increases chilling tolerance in salvia miltiorrhiza bunge
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000200219
work_keys_str_mv	AT gaolu calciumchlorideprimingincreaseschillingtoleranceinsalviamiltiorrhizabunge AT yanmin calciumchlorideprimingincreaseschillingtoleranceinsalviamiltiorrhizabunge
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Calcium chloride priming increases chilling tolerance in Salvia miltiorrhiza Bunge

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