Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker

Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker

ABSTRACT Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae is one of the major hindrances in rice (Oryza sativa L.) production across the world including Malaysia. Therefore, the development of disease-resistant varieties remains a very economical and effective method of controllin...

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Autores principales: Zuki,Zakiah Mohd, Rafii,Mohd Y., Ramli,Asfaliza, Oladosu,Yusuff, Latif,Mohammad Abdul, Sijam,Kamaruzaman, Ismail,Mohd Razi, Sarif,Hamidah Mohd
Lenguaje:English
Publicado: Instituto de Investigaciones Agropecuarias, INIA 2020
Materias:
Disease resistance
F2 bulk population
Oryza sativa
rice bacterial leaf blight
simple sequence repeat
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000200227
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spelling oai:scielo:S0718-583920200002002272020-11-30Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR markerZuki,Zakiah MohdRafii,Mohd Y.Ramli,AsfalizaOladosu,YusuffLatif,Mohammad AbdulSijam,KamaruzamanIsmail,Mohd RaziSarif,Hamidah Mohd Disease resistance F2 bulk population Oryza sativa rice bacterial leaf blight simple sequence repeat ABSTRACT Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae is one of the major hindrances in rice (Oryza sativa L.) production across the world including Malaysia. Therefore, the development of disease-resistant varieties remains a very economical and effective method of controlling BLB in rice. Based on this background, this study was conducted to analyze segregation pattern of simple-sequence repeat (SSR) markers associated with BLB resistance genes in an F2 bulk population derived from a resistant variety (IRBB60) and a susceptible variety (MR219). Out of 129 simple sequence repeat (SSR) markers screened, 18 distinct polymorphism markers including R-gene based markers were used to screen 345 F2 progenies for resistance to BLB. Among the polymorphic markers, the chi-square analyses showed that 15 SSR markers (i.e. RM13 (xa5), RM21 (Xa21), RM122 (xa5), RM153 (xa5), RM164 (Xa13), RM206 (Xa10), RM5509, RM20B, RM25, RM163, RM169, RM218, RM267, RM276, and RM334) had a segregation ratio of 1:2:1 for a single gene model (df = 2.0, p ≤ 0.05). For phenotypic ratio, the F2 population segregated in ratio 3:1 (R:S) for resistant and susceptible plants, respectively. This indicated that resistance to BLB caused by pathotype X. oryzae pv. oryzae (Xoo) in the ‘MR219’ × ‘IRBB60’ F2 population is controlled by single dominant genes. The result presented in this study will help breeders to further breeding research in rice by enabling selection based on the genotype rather than on the phenotype. Similarly, the markers reported in this study will serve as a valuable tool for marker-aided selection for BLB resistance gene.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.80 n.2 20202020-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000200227en10.4067/S0718-58392020000200227
institution Scielo Chile
collection Scielo Chile
language English
topic Disease resistance
F2 bulk population
Oryza sativa
rice bacterial leaf blight
simple sequence repeat
spellingShingle Disease resistance
F2 bulk population
Oryza sativa
rice bacterial leaf blight
simple sequence repeat
Zuki,Zakiah Mohd
Rafii,Mohd Y.
Ramli,Asfaliza
Oladosu,Yusuff
Latif,Mohammad Abdul
Sijam,Kamaruzaman
Ismail,Mohd Razi
Sarif,Hamidah Mohd
Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker
description ABSTRACT Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae is one of the major hindrances in rice (Oryza sativa L.) production across the world including Malaysia. Therefore, the development of disease-resistant varieties remains a very economical and effective method of controlling BLB in rice. Based on this background, this study was conducted to analyze segregation pattern of simple-sequence repeat (SSR) markers associated with BLB resistance genes in an F2 bulk population derived from a resistant variety (IRBB60) and a susceptible variety (MR219). Out of 129 simple sequence repeat (SSR) markers screened, 18 distinct polymorphism markers including R-gene based markers were used to screen 345 F2 progenies for resistance to BLB. Among the polymorphic markers, the chi-square analyses showed that 15 SSR markers (i.e. RM13 (xa5), RM21 (Xa21), RM122 (xa5), RM153 (xa5), RM164 (Xa13), RM206 (Xa10), RM5509, RM20B, RM25, RM163, RM169, RM218, RM267, RM276, and RM334) had a segregation ratio of 1:2:1 for a single gene model (df = 2.0, p ≤ 0.05). For phenotypic ratio, the F2 population segregated in ratio 3:1 (R:S) for resistant and susceptible plants, respectively. This indicated that resistance to BLB caused by pathotype X. oryzae pv. oryzae (Xoo) in the ‘MR219’ × ‘IRBB60’ F2 population is controlled by single dominant genes. The result presented in this study will help breeders to further breeding research in rice by enabling selection based on the genotype rather than on the phenotype. Similarly, the markers reported in this study will serve as a valuable tool for marker-aided selection for BLB resistance gene.
author Zuki,Zakiah Mohd
Rafii,Mohd Y.
Ramli,Asfaliza
Oladosu,Yusuff
Latif,Mohammad Abdul
Sijam,Kamaruzaman
Ismail,Mohd Razi
Sarif,Hamidah Mohd
author_facet Zuki,Zakiah Mohd
Rafii,Mohd Y.
Ramli,Asfaliza
Oladosu,Yusuff
Latif,Mohammad Abdul
Sijam,Kamaruzaman
Ismail,Mohd Razi
Sarif,Hamidah Mohd
author_sort Zuki,Zakiah Mohd
title Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker
title_short Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker
title_full Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker
title_fullStr Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker
title_full_unstemmed Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker
title_sort segregation analysis for bacterial leaf blight disease resistance genes in rice ‘mr219’ using ssr marker
publisher Instituto de Investigaciones Agropecuarias, INIA
publishDate 2020
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000200227
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