Differential response of denitrifying community to the application of green manure and reduced chemical fertilizer in a paddy soil

ABSTRACT Denitrification could lead to N loss from agricultural soil resulting in a low rate of N availability by crops. We investigated the response of nirK and nirS denitrifiers in a paddy soil to the incorporation of green manure and reduced chemical fertilizer. Soil samples were collected from p...

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Autores principales:	Fang,Yu, Wang,Fei, Jia,Xianbo, Zhang,Hui, Lin,Chenqiang, Chen,Longjun, Chen,Jichen
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2020
Materias:	Astragalus sinicus green manure nirK nirS reduced chemical fertilizer
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000300393
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spelling	oai:scielo:S0718-583920200003003932020-08-18Differential response of denitrifying community to the application of green manure and reduced chemical fertilizer in a paddy soilFang,YuWang,FeiJia,XianboZhang,HuiLin,ChenqiangChen,LongjunChen,Jichen Astragalus sinicus green manure nirK nirS reduced chemical fertilizer ABSTRACT Denitrification could lead to N loss from agricultural soil resulting in a low rate of N availability by crops. We investigated the response of nirK and nirS denitrifiers in a paddy soil to the incorporation of green manure and reduced chemical fertilizer. Soil samples were collected from plots of a short-term fertilization experiment initiated in 2009. The treatments were no chemical fertilizer, no Chinese milk vetch (Astragalus sinicus L., CK), chemical fertilizer (NPK), Chinese milk vetch (MV) plus 80% chemical fertilizer (MF80), MV plus 60% chemical fertilizer (MF60) and MV plus 40% chemical fertilizer (MF40). Abundance and community composition of nirK and nirS denitrifiers were analyzed using quantitative PCR and Miseq sequencing. Reduced chemical fertilizer did not reduce content of total N and available N in soils amended with green manure. Abundances of nirK and nirS genes in different treatments were 1.04 ×108 to 4.89×108 and 1.22 ×107 to 7.04 ×107 copies g-1 soil, respectively, which were significantly higher in NPK treatment than those in soils with green manure. NirK abundance was positively correlated with the potential denitrifying activity (PDA) (r2 = 0.827, p < 0.01). Green manure combination with reduced chemical fertilizer significantly changed the community structure of nirK denitrifiers but not nirS denitrifiers relative to soils amended with chemical fertilizer and unfertilized soil. Shifts of community structure of nirK denitrifiers were closely associated with soil organic matter (r2 = 0.623, p = 0.003), available N (r2 = 0.507, p = 0.01), pH (r2 = 0.661, p = 0.006), and PDA (r2 = 0.633, p = 0.005). In conclusion, nirK-type denitrifying community was more sensitive to the incorporation of green manure and reduced chemical fertilizer and they played a more important role in the denitrification process in this study.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.80 n.3 20202020-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000300393en10.4067/S0718-58392020000300393
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Astragalus sinicus green manure nirK nirS reduced chemical fertilizer
spellingShingle	Astragalus sinicus green manure nirK nirS reduced chemical fertilizer Fang,Yu Wang,Fei Jia,Xianbo Zhang,Hui Lin,Chenqiang Chen,Longjun Chen,Jichen Differential response of denitrifying community to the application of green manure and reduced chemical fertilizer in a paddy soil
description	ABSTRACT Denitrification could lead to N loss from agricultural soil resulting in a low rate of N availability by crops. We investigated the response of nirK and nirS denitrifiers in a paddy soil to the incorporation of green manure and reduced chemical fertilizer. Soil samples were collected from plots of a short-term fertilization experiment initiated in 2009. The treatments were no chemical fertilizer, no Chinese milk vetch (Astragalus sinicus L., CK), chemical fertilizer (NPK), Chinese milk vetch (MV) plus 80% chemical fertilizer (MF80), MV plus 60% chemical fertilizer (MF60) and MV plus 40% chemical fertilizer (MF40). Abundance and community composition of nirK and nirS denitrifiers were analyzed using quantitative PCR and Miseq sequencing. Reduced chemical fertilizer did not reduce content of total N and available N in soils amended with green manure. Abundances of nirK and nirS genes in different treatments were 1.04 ×108 to 4.89×108 and 1.22 ×107 to 7.04 ×107 copies g-1 soil, respectively, which were significantly higher in NPK treatment than those in soils with green manure. NirK abundance was positively correlated with the potential denitrifying activity (PDA) (r2 = 0.827, p < 0.01). Green manure combination with reduced chemical fertilizer significantly changed the community structure of nirK denitrifiers but not nirS denitrifiers relative to soils amended with chemical fertilizer and unfertilized soil. Shifts of community structure of nirK denitrifiers were closely associated with soil organic matter (r2 = 0.623, p = 0.003), available N (r2 = 0.507, p = 0.01), pH (r2 = 0.661, p = 0.006), and PDA (r2 = 0.633, p = 0.005). In conclusion, nirK-type denitrifying community was more sensitive to the incorporation of green manure and reduced chemical fertilizer and they played a more important role in the denitrification process in this study.
author	Fang,Yu Wang,Fei Jia,Xianbo Zhang,Hui Lin,Chenqiang Chen,Longjun Chen,Jichen
author_facet	Fang,Yu Wang,Fei Jia,Xianbo Zhang,Hui Lin,Chenqiang Chen,Longjun Chen,Jichen
author_sort	Fang,Yu
title	Differential response of denitrifying community to the application of green manure and reduced chemical fertilizer in a paddy soil
title_short	Differential response of denitrifying community to the application of green manure and reduced chemical fertilizer in a paddy soil
title_full	Differential response of denitrifying community to the application of green manure and reduced chemical fertilizer in a paddy soil
title_fullStr	Differential response of denitrifying community to the application of green manure and reduced chemical fertilizer in a paddy soil
title_full_unstemmed	Differential response of denitrifying community to the application of green manure and reduced chemical fertilizer in a paddy soil
title_sort	differential response of denitrifying community to the application of green manure and reduced chemical fertilizer in a paddy soil
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000300393
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Differential response of denitrifying community to the application of green manure and reduced chemical fertilizer in a paddy soil

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