Establishment of callus culture of Melientha suavis Pierre

ABSTRACT Melientha sauvis Pierre is an edible wild tree in Thailand. Local communities in Thailand harvest new shoot of the plant by burning the forest to stimulate it, a process that destroys the forest. This study was aimed at developing a methodology for callus culture production of M. suavis. Ca...

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Autores principales: Premjet,Duangporn, Obeng,Abraham K., Premjet,Siripong
Lenguaje:English
Publicado: Instituto de Investigaciones Agropecuarias, INIA 2020
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000300459
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spelling oai:scielo:S0718-583920200003004592020-08-18Establishment of callus culture of Melientha suavis PierrePremjet,DuangpornObeng,Abraham K.Premjet,Siripong 6-Benzylaminopurine callus cultures 2,4-dichlorophenoxyacetic acid pak wan pa ABSTRACT Melientha sauvis Pierre is an edible wild tree in Thailand. Local communities in Thailand harvest new shoot of the plant by burning the forest to stimulate it, a process that destroys the forest. This study was aimed at developing a methodology for callus culture production of M. suavis. Callus was induced from leaf, young stem, and shoot tip of M. suavis on Murashige and Skoog (MS) basal medium supplemented with various formulations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (6-BA). Callus produced from young stem explants appeared faster (14 d). Maximum callus growth (40%) and fresh weight (5.56 g) were observed in young stem explant cultured on MS medium containing 2 mg L-1 2,4-D. The findings of this work contribute to large scale micropropagation or bioactive compound production in M. suavis.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.80 n.3 20202020-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000300459en10.4067/S0718-58392020000300459
institution Scielo Chile
collection Scielo Chile
language English
topic 6-Benzylaminopurine
callus cultures
2,4-dichlorophenoxyacetic acid
pak wan pa
spellingShingle 6-Benzylaminopurine
callus cultures
2,4-dichlorophenoxyacetic acid
pak wan pa
Premjet,Duangporn
Obeng,Abraham K.
Premjet,Siripong
Establishment of callus culture of Melientha suavis Pierre
description ABSTRACT Melientha sauvis Pierre is an edible wild tree in Thailand. Local communities in Thailand harvest new shoot of the plant by burning the forest to stimulate it, a process that destroys the forest. This study was aimed at developing a methodology for callus culture production of M. suavis. Callus was induced from leaf, young stem, and shoot tip of M. suavis on Murashige and Skoog (MS) basal medium supplemented with various formulations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (6-BA). Callus produced from young stem explants appeared faster (14 d). Maximum callus growth (40%) and fresh weight (5.56 g) were observed in young stem explant cultured on MS medium containing 2 mg L-1 2,4-D. The findings of this work contribute to large scale micropropagation or bioactive compound production in M. suavis.
author Premjet,Duangporn
Obeng,Abraham K.
Premjet,Siripong
author_facet Premjet,Duangporn
Obeng,Abraham K.
Premjet,Siripong
author_sort Premjet,Duangporn
title Establishment of callus culture of Melientha suavis Pierre
title_short Establishment of callus culture of Melientha suavis Pierre
title_full Establishment of callus culture of Melientha suavis Pierre
title_fullStr Establishment of callus culture of Melientha suavis Pierre
title_full_unstemmed Establishment of callus culture of Melientha suavis Pierre
title_sort establishment of callus culture of melientha suavis pierre
publisher Instituto de Investigaciones Agropecuarias, INIA
publishDate 2020
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000300459
work_keys_str_mv AT premjetduangporn establishmentofcalluscultureofmelienthasuavispierre
AT obengabrahamk establishmentofcalluscultureofmelienthasuavispierre
AT premjetsiripong establishmentofcalluscultureofmelienthasuavispierre
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