Establishment of callus culture of Melientha suavis Pierre
ABSTRACT Melientha sauvis Pierre is an edible wild tree in Thailand. Local communities in Thailand harvest new shoot of the plant by burning the forest to stimulate it, a process that destroys the forest. This study was aimed at developing a methodology for callus culture production of M. suavis. Ca...
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Instituto de Investigaciones Agropecuarias, INIA
2020
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oai:scielo:S0718-583920200003004592020-08-18Establishment of callus culture of Melientha suavis PierrePremjet,DuangpornObeng,Abraham K.Premjet,Siripong 6-Benzylaminopurine callus cultures 2,4-dichlorophenoxyacetic acid pak wan pa ABSTRACT Melientha sauvis Pierre is an edible wild tree in Thailand. Local communities in Thailand harvest new shoot of the plant by burning the forest to stimulate it, a process that destroys the forest. This study was aimed at developing a methodology for callus culture production of M. suavis. Callus was induced from leaf, young stem, and shoot tip of M. suavis on Murashige and Skoog (MS) basal medium supplemented with various formulations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (6-BA). Callus produced from young stem explants appeared faster (14 d). Maximum callus growth (40%) and fresh weight (5.56 g) were observed in young stem explant cultured on MS medium containing 2 mg L-1 2,4-D. The findings of this work contribute to large scale micropropagation or bioactive compound production in M. suavis.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.80 n.3 20202020-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000300459en10.4067/S0718-58392020000300459 |
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English |
topic |
6-Benzylaminopurine callus cultures 2,4-dichlorophenoxyacetic acid pak wan pa |
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6-Benzylaminopurine callus cultures 2,4-dichlorophenoxyacetic acid pak wan pa Premjet,Duangporn Obeng,Abraham K. Premjet,Siripong Establishment of callus culture of Melientha suavis Pierre |
description |
ABSTRACT Melientha sauvis Pierre is an edible wild tree in Thailand. Local communities in Thailand harvest new shoot of the plant by burning the forest to stimulate it, a process that destroys the forest. This study was aimed at developing a methodology for callus culture production of M. suavis. Callus was induced from leaf, young stem, and shoot tip of M. suavis on Murashige and Skoog (MS) basal medium supplemented with various formulations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (6-BA). Callus produced from young stem explants appeared faster (14 d). Maximum callus growth (40%) and fresh weight (5.56 g) were observed in young stem explant cultured on MS medium containing 2 mg L-1 2,4-D. The findings of this work contribute to large scale micropropagation or bioactive compound production in M. suavis. |
author |
Premjet,Duangporn Obeng,Abraham K. Premjet,Siripong |
author_facet |
Premjet,Duangporn Obeng,Abraham K. Premjet,Siripong |
author_sort |
Premjet,Duangporn |
title |
Establishment of callus culture of Melientha suavis Pierre |
title_short |
Establishment of callus culture of Melientha suavis Pierre |
title_full |
Establishment of callus culture of Melientha suavis Pierre |
title_fullStr |
Establishment of callus culture of Melientha suavis Pierre |
title_full_unstemmed |
Establishment of callus culture of Melientha suavis Pierre |
title_sort |
establishment of callus culture of melientha suavis pierre |
publisher |
Instituto de Investigaciones Agropecuarias, INIA |
publishDate |
2020 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000300459 |
work_keys_str_mv |
AT premjetduangporn establishmentofcalluscultureofmelienthasuavispierre AT obengabrahamk establishmentofcalluscultureofmelienthasuavispierre AT premjetsiripong establishmentofcalluscultureofmelienthasuavispierre |
_version_ |
1714205402630979584 |