Micropropagation and germplasm conservation of four chickpea (Cicer arietinum L.) genotypes

ABSTRACT Genetic improvement of chickpea (Cicer arietinum L.), a recalcitrant crop, has been largely restricted owing to a lack of efficient regeneration methods. In this study, four chickpea genotypes (Giza 4, Giza 88, Giza 195, and Giza 531) were efficiently micropropagated using embryo axes and s...

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Autores principales:	Alghamdi,Salem S., Dewir,Yaser Hassan, Khan,Muhammad Altaf, Migdadi,Hussein, EL-Harty,Ehab H., Aldubai,Abdulhakim A., Al-Aizari,Ahmed A.
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2020
Materias:	Embryo recalcitrant crops regeneration rooting synthetic seeds
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000400487
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spelling	oai:scielo:S0718-583920200004004872020-11-22Micropropagation and germplasm conservation of four chickpea (Cicer arietinum L.) genotypesAlghamdi,Salem S.Dewir,Yaser HassanKhan,Muhammad AltafMigdadi,HusseinEL-Harty,Ehab H.Aldubai,Abdulhakim A.Al-Aizari,Ahmed A. Embryo recalcitrant crops regeneration rooting synthetic seeds ABSTRACT Genetic improvement of chickpea (Cicer arietinum L.), a recalcitrant crop, has been largely restricted owing to a lack of efficient regeneration methods. In this study, four chickpea genotypes (Giza 4, Giza 88, Giza 195, and Giza 531) were efficiently micropropagated using embryo axes and synthetic seeds developed using an encapsulation technique. Multiple shoots grew from embryo explants using 2 and 4 mg L-1 benzylaminopurine (BAP). The number of shoots for Giza 195 explants using 4 and 6 mg L-1 BAP were 5.8 and 6.2, respectively. The elongated shoots were transferred to two indole- 3-butyric acid (IBA) concentrations (50 and 100 mmol L-1) for root induction. A high rooting percentage (67%-100%) followed by successful acclimatization (70%-75%) was obtained for shoots dipped in 50 mmol L-1 IBA and cultured into an MS liquid medium. Apical buds from in vitro shoots were encapsulated in sodium alginate along with calcium chloride to produce synthetic seeds, which were successfully stored at 4 °C for 5 mo. Giza 195 and Giza 531 were better conserved than Giza 4 and Giza 88, with 70%-75% regrowth and recovery rates. These results confirmed an efficient regeneration protocol and synthetic seed production, which could be utilized for genetic transformations and crop improvement.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.80 n.4 20202020-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000400487en10.4067/S0718-58392020000400487
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Embryo recalcitrant crops regeneration rooting synthetic seeds
spellingShingle	Embryo recalcitrant crops regeneration rooting synthetic seeds Alghamdi,Salem S. Dewir,Yaser Hassan Khan,Muhammad Altaf Migdadi,Hussein EL-Harty,Ehab H. Aldubai,Abdulhakim A. Al-Aizari,Ahmed A. Micropropagation and germplasm conservation of four chickpea (Cicer arietinum L.) genotypes
description	ABSTRACT Genetic improvement of chickpea (Cicer arietinum L.), a recalcitrant crop, has been largely restricted owing to a lack of efficient regeneration methods. In this study, four chickpea genotypes (Giza 4, Giza 88, Giza 195, and Giza 531) were efficiently micropropagated using embryo axes and synthetic seeds developed using an encapsulation technique. Multiple shoots grew from embryo explants using 2 and 4 mg L-1 benzylaminopurine (BAP). The number of shoots for Giza 195 explants using 4 and 6 mg L-1 BAP were 5.8 and 6.2, respectively. The elongated shoots were transferred to two indole- 3-butyric acid (IBA) concentrations (50 and 100 mmol L-1) for root induction. A high rooting percentage (67%-100%) followed by successful acclimatization (70%-75%) was obtained for shoots dipped in 50 mmol L-1 IBA and cultured into an MS liquid medium. Apical buds from in vitro shoots were encapsulated in sodium alginate along with calcium chloride to produce synthetic seeds, which were successfully stored at 4 °C for 5 mo. Giza 195 and Giza 531 were better conserved than Giza 4 and Giza 88, with 70%-75% regrowth and recovery rates. These results confirmed an efficient regeneration protocol and synthetic seed production, which could be utilized for genetic transformations and crop improvement.
author	Alghamdi,Salem S. Dewir,Yaser Hassan Khan,Muhammad Altaf Migdadi,Hussein EL-Harty,Ehab H. Aldubai,Abdulhakim A. Al-Aizari,Ahmed A.
author_facet	Alghamdi,Salem S. Dewir,Yaser Hassan Khan,Muhammad Altaf Migdadi,Hussein EL-Harty,Ehab H. Aldubai,Abdulhakim A. Al-Aizari,Ahmed A.
author_sort	Alghamdi,Salem S.
title	Micropropagation and germplasm conservation of four chickpea (Cicer arietinum L.) genotypes
title_short	Micropropagation and germplasm conservation of four chickpea (Cicer arietinum L.) genotypes
title_full	Micropropagation and germplasm conservation of four chickpea (Cicer arietinum L.) genotypes
title_fullStr	Micropropagation and germplasm conservation of four chickpea (Cicer arietinum L.) genotypes
title_full_unstemmed	Micropropagation and germplasm conservation of four chickpea (Cicer arietinum L.) genotypes
title_sort	micropropagation and germplasm conservation of four chickpea (cicer arietinum l.) genotypes
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000400487
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Micropropagation and germplasm conservation of four chickpea (Cicer arietinum L.) genotypes

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