Assessing the genetic diversity of Dioscorea alata and related species from Colombia through inter-simple sequence repeat (ISSR) markers

ABSTRACT Dioscorea, commonly known as yam, is an economically, socially, and culturally-relevant tuber cropin many tropical countries. This study aimed to characterize the genetic diversity of Dioscorea alata L. and related species from Colombia (42 D. alata L., 6 D. bulbifera L., 3 D. rotundata Poi...

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Autores principales:	Castañeda-Cardona,Christian C., Morillo-Coronado,Yacenia, Morillo,Ana C.
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2020
Materias:	Molecular markers ploidy population structure yam
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000400608
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spelling	oai:scielo:S0718-583920200004006082020-11-22Assessing the genetic diversity of Dioscorea alata and related species from Colombia through inter-simple sequence repeat (ISSR) markersCastañeda-Cardona,Christian C.Morillo-Coronado,YaceniaMorillo,Ana C. Molecular markers ploidy population structure yam ABSTRACT Dioscorea, commonly known as yam, is an economically, socially, and culturally-relevant tuber cropin many tropical countries. This study aimed to characterize the genetic diversity of Dioscorea alata L. and related species from Colombia (42 D. alata L., 6 D. bulbifera L., 3 D. rotundata Poir., and 3 D. trifida L. f.) through seven inter- simple sequence repeat (ISSR) markers. The plant material was collected in five departments of Colombia. The seven ISSR generated 164 bands that showed high polymorphism (83.62%). Nei-Li genetic diversity coefficient (0.66) and Shannon information index (0.2636) revealed a high level of genetic diversity. The 54 genotypes were grouped into six groups according to geographic location but not by species. We found moderate genetic differentiation (Gst = 0.13) and high gene flow (Nm = 2.7367). The analysis of molecular variance (AMOVA) showed higher variation (86%) within groups than among groups (14%). Our results suggest that the high genetic diversity in yam in Colombia can be exploited in future work on crop improvement.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.80 n.4 20202020-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000400608en10.4067/S0718-58392020000400608
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Molecular markers ploidy population structure yam
spellingShingle	Molecular markers ploidy population structure yam Castañeda-Cardona,Christian C. Morillo-Coronado,Yacenia Morillo,Ana C. Assessing the genetic diversity of Dioscorea alata and related species from Colombia through inter-simple sequence repeat (ISSR) markers
description	ABSTRACT Dioscorea, commonly known as yam, is an economically, socially, and culturally-relevant tuber cropin many tropical countries. This study aimed to characterize the genetic diversity of Dioscorea alata L. and related species from Colombia (42 D. alata L., 6 D. bulbifera L., 3 D. rotundata Poir., and 3 D. trifida L. f.) through seven inter- simple sequence repeat (ISSR) markers. The plant material was collected in five departments of Colombia. The seven ISSR generated 164 bands that showed high polymorphism (83.62%). Nei-Li genetic diversity coefficient (0.66) and Shannon information index (0.2636) revealed a high level of genetic diversity. The 54 genotypes were grouped into six groups according to geographic location but not by species. We found moderate genetic differentiation (Gst = 0.13) and high gene flow (Nm = 2.7367). The analysis of molecular variance (AMOVA) showed higher variation (86%) within groups than among groups (14%). Our results suggest that the high genetic diversity in yam in Colombia can be exploited in future work on crop improvement.
author	Castañeda-Cardona,Christian C. Morillo-Coronado,Yacenia Morillo,Ana C.
author_facet	Castañeda-Cardona,Christian C. Morillo-Coronado,Yacenia Morillo,Ana C.
author_sort	Castañeda-Cardona,Christian C.
title	Assessing the genetic diversity of Dioscorea alata and related species from Colombia through inter-simple sequence repeat (ISSR) markers
title_short	Assessing the genetic diversity of Dioscorea alata and related species from Colombia through inter-simple sequence repeat (ISSR) markers
title_full	Assessing the genetic diversity of Dioscorea alata and related species from Colombia through inter-simple sequence repeat (ISSR) markers
title_fullStr	Assessing the genetic diversity of Dioscorea alata and related species from Colombia through inter-simple sequence repeat (ISSR) markers
title_full_unstemmed	Assessing the genetic diversity of Dioscorea alata and related species from Colombia through inter-simple sequence repeat (ISSR) markers
title_sort	assessing the genetic diversity of dioscorea alata and related species from colombia through inter-simple sequence repeat (issr) markers
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000400608
work_keys_str_mv	AT castanedacardonachristianc assessingthegeneticdiversityofdioscoreaalataandrelatedspeciesfromcolombiathroughintersimplesequencerepeatissrmarkers AT morillocoronadoyacenia assessingthegeneticdiversityofdioscoreaalataandrelatedspeciesfromcolombiathroughintersimplesequencerepeatissrmarkers AT morilloanac assessingthegeneticdiversityofdioscoreaalataandrelatedspeciesfromcolombiathroughintersimplesequencerepeatissrmarkers
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Assessing the genetic diversity of Dioscorea alata and related species from Colombia through inter-simple sequence repeat (ISSR) markers

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