Assessment of genetic diversity in Tunisian populations of Medicago polymorpha based on SSR markers

ABSTRACT Medicago polymorpha L. is a herbaceous legume that can be a useful pasture crop, especially in Mediterranean climates. This study aimed to analyze the genetic variation in five populations of M. polymorpha collected from different eco- geographic regions in Tunisia using eight SSR markers....

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Autores principales:	Haddoudi,Loua, Hdira,Sabrine, Cheikh,Najah Ben, Mahjoub,Asma, Abdelly,Chedly, Ludidi,Ndiko, Badri,Mounawer
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2021
Materias:	Gene flow FST Medicago polymorpha SSR markers Tunisian populations.
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392021000100053
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spelling	oai:scielo:S0718-583920210001000532021-02-24Assessment of genetic diversity in Tunisian populations of Medicago polymorpha based on SSR markersHaddoudi,LouaHdira,SabrineCheikh,Najah BenMahjoub,AsmaAbdelly,ChedlyLudidi,NdikoBadri,Mounawer Gene flow FST Medicago polymorpha SSR markers Tunisian populations. ABSTRACT Medicago polymorpha L. is a herbaceous legume that can be a useful pasture crop, especially in Mediterranean climates. This study aimed to analyze the genetic variation in five populations of M. polymorpha collected from different eco- geographic regions in Tunisia using eight SSR markers. The transferability of 112 SSR markers distributed on the eight chromosomes of M. truncatula Gaertn. showed that 50 SSR markers could be amplified in M. polymorpha. Among these 50 SSR markers, eight (8) markers were polymorphous. A high level of polymorphism (126 polymorphic alleles with an average of 5.3 alleles per locus) and a moderate level of genetic diversity were found in all the studied populations, with observed and expected heterozygosities averaging between 0 and 0.69, respectively. Results from analysis of molecular variance (AMOVA) revealed that the most variation was found within populations (76%). Moderate levels of population differentiation (FST = 0.12 to 0.19) accompanied by a high rate of gene flow between populations (Nm = 1.08 to 1.83) were recorded. This molecular differentiation (FST) was not dependent on geographical distances (r = 0.395, p = 0.524), suggesting that studied populations are not geographically isolated. Our results showed that studied populations were clustered into three groups. A first group is formed by the populations of El Kef (TNP7) and Bulla Regia (TNP9), a second group of Enfidha (TNP1) and a third group of Soliman (TNP8) and Mateur (TNP11). Results obtained in our study could be helpful for breeders considering introduction of some lines of this species into M. polymorpha breeding programs.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.81 n.1 20212021-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392021000100053en10.4067/S0718-58392021000100053
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Gene flow FST Medicago polymorpha SSR markers Tunisian populations.
spellingShingle	Gene flow FST Medicago polymorpha SSR markers Tunisian populations. Haddoudi,Loua Hdira,Sabrine Cheikh,Najah Ben Mahjoub,Asma Abdelly,Chedly Ludidi,Ndiko Badri,Mounawer Assessment of genetic diversity in Tunisian populations of Medicago polymorpha based on SSR markers
description	ABSTRACT Medicago polymorpha L. is a herbaceous legume that can be a useful pasture crop, especially in Mediterranean climates. This study aimed to analyze the genetic variation in five populations of M. polymorpha collected from different eco- geographic regions in Tunisia using eight SSR markers. The transferability of 112 SSR markers distributed on the eight chromosomes of M. truncatula Gaertn. showed that 50 SSR markers could be amplified in M. polymorpha. Among these 50 SSR markers, eight (8) markers were polymorphous. A high level of polymorphism (126 polymorphic alleles with an average of 5.3 alleles per locus) and a moderate level of genetic diversity were found in all the studied populations, with observed and expected heterozygosities averaging between 0 and 0.69, respectively. Results from analysis of molecular variance (AMOVA) revealed that the most variation was found within populations (76%). Moderate levels of population differentiation (FST = 0.12 to 0.19) accompanied by a high rate of gene flow between populations (Nm = 1.08 to 1.83) were recorded. This molecular differentiation (FST) was not dependent on geographical distances (r = 0.395, p = 0.524), suggesting that studied populations are not geographically isolated. Our results showed that studied populations were clustered into three groups. A first group is formed by the populations of El Kef (TNP7) and Bulla Regia (TNP9), a second group of Enfidha (TNP1) and a third group of Soliman (TNP8) and Mateur (TNP11). Results obtained in our study could be helpful for breeders considering introduction of some lines of this species into M. polymorpha breeding programs.
author	Haddoudi,Loua Hdira,Sabrine Cheikh,Najah Ben Mahjoub,Asma Abdelly,Chedly Ludidi,Ndiko Badri,Mounawer
author_facet	Haddoudi,Loua Hdira,Sabrine Cheikh,Najah Ben Mahjoub,Asma Abdelly,Chedly Ludidi,Ndiko Badri,Mounawer
author_sort	Haddoudi,Loua
title	Assessment of genetic diversity in Tunisian populations of Medicago polymorpha based on SSR markers
title_short	Assessment of genetic diversity in Tunisian populations of Medicago polymorpha based on SSR markers
title_full	Assessment of genetic diversity in Tunisian populations of Medicago polymorpha based on SSR markers
title_fullStr	Assessment of genetic diversity in Tunisian populations of Medicago polymorpha based on SSR markers
title_full_unstemmed	Assessment of genetic diversity in Tunisian populations of Medicago polymorpha based on SSR markers
title_sort	assessment of genetic diversity in tunisian populations of medicago polymorpha based on ssr markers
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392021000100053
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Assessment of genetic diversity in Tunisian populations of Medicago polymorpha based on SSR markers

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