Molecular profiling of sweet cherry cultivars present in Chile using polymorphic microsatellite markers

ABSTRACT Sweet cherry (Prunus avium (L.) L.) is one of the most important fruit crops of temperate climates. In Chile, the actual planted area is over 42 000 ha that produce over 260 000 t yearly. The accurate identification of sweet cherry cultivars is key for processes involved both in breeding ne...

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Autores principales:	Guajardo,Verónica, Muñoz,Carlos, Hinrichsen,Patricio
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2021
Materias:	Cultivar identification fingerprinting germplasm molecular markers Prunus avium SSR
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392021000300326
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spelling	oai:scielo:S0718-583920210003003262021-08-16Molecular profiling of sweet cherry cultivars present in Chile using polymorphic microsatellite markersGuajardo,VerónicaMuñoz,CarlosHinrichsen,Patricio Cultivar identification fingerprinting germplasm molecular markers Prunus avium SSR ABSTRACT Sweet cherry (Prunus avium (L.) L.) is one of the most important fruit crops of temperate climates. In Chile, the actual planted area is over 42 000 ha that produce over 260 000 t yearly. The accurate identification of sweet cherry cultivars is key for processes involved both in breeding new cultivars and along the production chain. In this study, we performed the molecular characterization of 87 sweet cherry genotypes cultivated in Chile, using nine microsatellite markers originally described for both peaches and sweet cherries. The analysis showed that 69 of these genotypes corresponded to unique cultivars, each harboring a unique allelic pattern. They could be differentiated using only five markers (BPPCT-037, BPPCT-039, BPPCT-040, PMS-30 and UCD-CH18). The remaining 19 genotypes could correspond to misidentified, mutated or even synonyms of the studied genotypes, since they have allelic patterns identical to one or more of the 69 individualized genotypes. Between 3 and 8 alleles per marker were identified, with a mean of 6, while the expected heterozygosity over the nine polymorphic loci averaged 0.72, ranging from 0.59 in UDP96-001 to 0.78 in BPPCT-040. Phylogenetic and population structure analyses showed that most cultivars were grouped according to their country of origin or the breeding program from where they were released, being also coincident with their presumed pedigrees. These results are the basis for a fingerprinting protocol, based on microsatellite markers, for sweet cherry cultivars.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.81 n.3 20212021-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392021000300326en10.4067/S0718-58392021000300326
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cultivar identification fingerprinting germplasm molecular markers Prunus avium SSR
spellingShingle	Cultivar identification fingerprinting germplasm molecular markers Prunus avium SSR Guajardo,Verónica Muñoz,Carlos Hinrichsen,Patricio Molecular profiling of sweet cherry cultivars present in Chile using polymorphic microsatellite markers
description	ABSTRACT Sweet cherry (Prunus avium (L.) L.) is one of the most important fruit crops of temperate climates. In Chile, the actual planted area is over 42 000 ha that produce over 260 000 t yearly. The accurate identification of sweet cherry cultivars is key for processes involved both in breeding new cultivars and along the production chain. In this study, we performed the molecular characterization of 87 sweet cherry genotypes cultivated in Chile, using nine microsatellite markers originally described for both peaches and sweet cherries. The analysis showed that 69 of these genotypes corresponded to unique cultivars, each harboring a unique allelic pattern. They could be differentiated using only five markers (BPPCT-037, BPPCT-039, BPPCT-040, PMS-30 and UCD-CH18). The remaining 19 genotypes could correspond to misidentified, mutated or even synonyms of the studied genotypes, since they have allelic patterns identical to one or more of the 69 individualized genotypes. Between 3 and 8 alleles per marker were identified, with a mean of 6, while the expected heterozygosity over the nine polymorphic loci averaged 0.72, ranging from 0.59 in UDP96-001 to 0.78 in BPPCT-040. Phylogenetic and population structure analyses showed that most cultivars were grouped according to their country of origin or the breeding program from where they were released, being also coincident with their presumed pedigrees. These results are the basis for a fingerprinting protocol, based on microsatellite markers, for sweet cherry cultivars.
author	Guajardo,Verónica Muñoz,Carlos Hinrichsen,Patricio
author_facet	Guajardo,Verónica Muñoz,Carlos Hinrichsen,Patricio
author_sort	Guajardo,Verónica
title	Molecular profiling of sweet cherry cultivars present in Chile using polymorphic microsatellite markers
title_short	Molecular profiling of sweet cherry cultivars present in Chile using polymorphic microsatellite markers
title_full	Molecular profiling of sweet cherry cultivars present in Chile using polymorphic microsatellite markers
title_fullStr	Molecular profiling of sweet cherry cultivars present in Chile using polymorphic microsatellite markers
title_full_unstemmed	Molecular profiling of sweet cherry cultivars present in Chile using polymorphic microsatellite markers
title_sort	molecular profiling of sweet cherry cultivars present in chile using polymorphic microsatellite markers
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392021000300326
work_keys_str_mv	AT guajardoveronica molecularprofilingofsweetcherrycultivarspresentinchileusingpolymorphicmicrosatellitemarkers AT munozcarlos molecularprofilingofsweetcherrycultivarspresentinchileusingpolymorphicmicrosatellitemarkers AT hinrichsenpatricio molecularprofilingofsweetcherrycultivarspresentinchileusingpolymorphicmicrosatellitemarkers
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Molecular profiling of sweet cherry cultivars present in Chile using polymorphic microsatellite markers

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