Bacterial community associated with canker disease from sweet cherry orchards of central valley of Chile presents high resistance to copper

ABSTRACT The Chilean sweet cherry (Prunus avium (L.) L.) industry became the leading exporter worldwide. The bacterial canker is the most significant disease causing major economic losses. Pseudomonas syringae pv. syringae (Pss) is the only related pathovar recognized in Chile and it is mainly contr...

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Autores principales:	Beltrán,M. Francisca, Osorio,Valeria, Lemus,Gamalier, Millas,Paz, France,Andrés, Correa,Francisco, Sagredo,Boris
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2021
Materias:	Copper resistance Pseudomonas syringe pv. syringae identification sweet cherry
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392021000300378
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spelling	oai:scielo:S0718-583920210003003782021-08-16Bacterial community associated with canker disease from sweet cherry orchards of central valley of Chile presents high resistance to copperBeltrán,M. FranciscaOsorio,ValeriaLemus,GamalierMillas,PazFrance,AndrésCorrea,FranciscoSagredo,Boris Copper resistance Pseudomonas syringe pv. syringae identification sweet cherry ABSTRACT The Chilean sweet cherry (Prunus avium (L.) L.) industry became the leading exporter worldwide. The bacterial canker is the most significant disease causing major economic losses. Pseudomonas syringae pv. syringae (Pss) is the only related pathovar recognized in Chile and it is mainly controlled with Cu-based antimicrobial compounds (CBAC). Soil contamination and the decreasing efficacy of CBACs by the emergence of Cu-resistant bacterial strains threaten the long- term sustainability of sweet cherry production. This study aimed at characterizing the bacterial community associated with canker infection injuries by assessing Cu resistance in sweet cherry orchards in the O’Higgins Region of Chile. Eighty bacteria isolates were obtained from tissue that presented bacterial canker symptoms from cherry trees. We assessed the production of fluorescent pigments in Fe-deficient media, presence of the housekeeping genes rpoD and cts, and presence of syringomycin-producing genes syrb and syrD. Their pathogenic ability was evaluated on immature sweet cherry fruits and Cu resistance was determined as the minimum inhibitory concentration (MIC) using CuSO4. Only five isolates were Cu-susceptible (MIC < 0.8 mM), while 75 isolates exhibited different levels of Cu resistance (MIC > 0.8 mM). At least one gene of the copABCD operon/or and its regulatory genes were detected in 17 isolates, suggesting that most isolates likely have different mechanisms of Cu tolerance. Six isolates were identified as Pss, presenting different degrees of Cu resistance, but all presented at least one feature of the copABCD operon. A bacterial community that presents high Cu-resistance, probably under control of diverse genetic mechanisms, decreases the efficacy of CBAC.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.81 n.3 20212021-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392021000300378en10.4067/S0718-58392021000300378
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Copper resistance Pseudomonas syringe pv. syringae identification sweet cherry
spellingShingle	Copper resistance Pseudomonas syringe pv. syringae identification sweet cherry Beltrán,M. Francisca Osorio,Valeria Lemus,Gamalier Millas,Paz France,Andrés Correa,Francisco Sagredo,Boris Bacterial community associated with canker disease from sweet cherry orchards of central valley of Chile presents high resistance to copper
description	ABSTRACT The Chilean sweet cherry (Prunus avium (L.) L.) industry became the leading exporter worldwide. The bacterial canker is the most significant disease causing major economic losses. Pseudomonas syringae pv. syringae (Pss) is the only related pathovar recognized in Chile and it is mainly controlled with Cu-based antimicrobial compounds (CBAC). Soil contamination and the decreasing efficacy of CBACs by the emergence of Cu-resistant bacterial strains threaten the long- term sustainability of sweet cherry production. This study aimed at characterizing the bacterial community associated with canker infection injuries by assessing Cu resistance in sweet cherry orchards in the O’Higgins Region of Chile. Eighty bacteria isolates were obtained from tissue that presented bacterial canker symptoms from cherry trees. We assessed the production of fluorescent pigments in Fe-deficient media, presence of the housekeeping genes rpoD and cts, and presence of syringomycin-producing genes syrb and syrD. Their pathogenic ability was evaluated on immature sweet cherry fruits and Cu resistance was determined as the minimum inhibitory concentration (MIC) using CuSO4. Only five isolates were Cu-susceptible (MIC < 0.8 mM), while 75 isolates exhibited different levels of Cu resistance (MIC > 0.8 mM). At least one gene of the copABCD operon/or and its regulatory genes were detected in 17 isolates, suggesting that most isolates likely have different mechanisms of Cu tolerance. Six isolates were identified as Pss, presenting different degrees of Cu resistance, but all presented at least one feature of the copABCD operon. A bacterial community that presents high Cu-resistance, probably under control of diverse genetic mechanisms, decreases the efficacy of CBAC.
author	Beltrán,M. Francisca Osorio,Valeria Lemus,Gamalier Millas,Paz France,Andrés Correa,Francisco Sagredo,Boris
author_facet	Beltrán,M. Francisca Osorio,Valeria Lemus,Gamalier Millas,Paz France,Andrés Correa,Francisco Sagredo,Boris
author_sort	Beltrán,M. Francisca
title	Bacterial community associated with canker disease from sweet cherry orchards of central valley of Chile presents high resistance to copper
title_short	Bacterial community associated with canker disease from sweet cherry orchards of central valley of Chile presents high resistance to copper
title_full	Bacterial community associated with canker disease from sweet cherry orchards of central valley of Chile presents high resistance to copper
title_fullStr	Bacterial community associated with canker disease from sweet cherry orchards of central valley of Chile presents high resistance to copper
title_full_unstemmed	Bacterial community associated with canker disease from sweet cherry orchards of central valley of Chile presents high resistance to copper
title_sort	bacterial community associated with canker disease from sweet cherry orchards of central valley of chile presents high resistance to copper
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392021000300378
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Bacterial community associated with canker disease from sweet cherry orchards of central valley of Chile presents high resistance to copper

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