Pre-breeding for waxy proso millet by phenotyping and marker-assisted selection

ABSTRACT Proso millet (Panicum miliaceum L.) is important in some Kazakhstan food, this research objective was to develop pre- breeding waxy proso millet resource for breeding new varieties by using phenotyping and marker-assisted selection. The amylose content of the endosperm starch in a collectio...

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Autores principales:	Zhirnova,Irina, Rysbekova,Aiman, Dyussibayeva,Elmira, Zhakenova,Aiym, Amantaev,Bekzak, Hu,Yin-Gang, Feng,Bai-Li, Zhunusbayeva,Zhazira
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2021
Materias:	Amylose content glutinous variety hybridization Panicum miliaceum proso millet waxy gene
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392021000400518
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spelling	oai:scielo:S0718-583920210004005182021-10-27Pre-breeding for waxy proso millet by phenotyping and marker-assisted selectionZhirnova,IrinaRysbekova,AimanDyussibayeva,ElmiraZhakenova,AiymAmantaev,BekzakHu,Yin-GangFeng,Bai-LiZhunusbayeva,Zhazira Amylose content glutinous variety hybridization Panicum miliaceum proso millet waxy gene ABSTRACT Proso millet (Panicum miliaceum L.) is important in some Kazakhstan food, this research objective was to develop pre- breeding waxy proso millet resource for breeding new varieties by using phenotyping and marker-assisted selection. The amylose content of the endosperm starch in a collection of 18 proso millet cultivars widely used in northern Kazakhstan were evaluated and ranged from 14.6% to 34.8%. The introduced glutinous millet accessions of PI436626 (Lung Shu 18), PI436625 (Lung Shu 16) were selected to cross with local cultivars (Saratovskoe 6 and Pamyati Bersieva). The potassium iodide (KI) staining method and PCR analysis using the 9bF/15delRB primer were used to identify the waxy trait for selection of the F2-F3 generation. Seeds staining dark blue to black were scored as wild type, while waxy seeds were stained as pinkish or amber to reddish-brown color. The hybridological analysis of inheritance of the “waxy” trait suggested that the segregation ratios for waxy endosperm in the F2 and F3 populations was close to 15:1 of the caryopses with blue and brown colors. PCR analysis with the 9bF/15delRB primer confirmed that this specific primer flanks a 123 bp fragment in amylose samples, while in glutinous samples, this primer promotes the amplification of 108 bp long PCR product (15 nucleotides deletion). Finally, the waxy lines were obtained and could be used for further testing.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.81 n.4 20212021-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392021000400518en10.4067/S0718-58392021000400518
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Amylose content glutinous variety hybridization Panicum miliaceum proso millet waxy gene
spellingShingle	Amylose content glutinous variety hybridization Panicum miliaceum proso millet waxy gene Zhirnova,Irina Rysbekova,Aiman Dyussibayeva,Elmira Zhakenova,Aiym Amantaev,Bekzak Hu,Yin-Gang Feng,Bai-Li Zhunusbayeva,Zhazira Pre-breeding for waxy proso millet by phenotyping and marker-assisted selection
description	ABSTRACT Proso millet (Panicum miliaceum L.) is important in some Kazakhstan food, this research objective was to develop pre- breeding waxy proso millet resource for breeding new varieties by using phenotyping and marker-assisted selection. The amylose content of the endosperm starch in a collection of 18 proso millet cultivars widely used in northern Kazakhstan were evaluated and ranged from 14.6% to 34.8%. The introduced glutinous millet accessions of PI436626 (Lung Shu 18), PI436625 (Lung Shu 16) were selected to cross with local cultivars (Saratovskoe 6 and Pamyati Bersieva). The potassium iodide (KI) staining method and PCR analysis using the 9bF/15delRB primer were used to identify the waxy trait for selection of the F2-F3 generation. Seeds staining dark blue to black were scored as wild type, while waxy seeds were stained as pinkish or amber to reddish-brown color. The hybridological analysis of inheritance of the “waxy” trait suggested that the segregation ratios for waxy endosperm in the F2 and F3 populations was close to 15:1 of the caryopses with blue and brown colors. PCR analysis with the 9bF/15delRB primer confirmed that this specific primer flanks a 123 bp fragment in amylose samples, while in glutinous samples, this primer promotes the amplification of 108 bp long PCR product (15 nucleotides deletion). Finally, the waxy lines were obtained and could be used for further testing.
author	Zhirnova,Irina Rysbekova,Aiman Dyussibayeva,Elmira Zhakenova,Aiym Amantaev,Bekzak Hu,Yin-Gang Feng,Bai-Li Zhunusbayeva,Zhazira
author_facet	Zhirnova,Irina Rysbekova,Aiman Dyussibayeva,Elmira Zhakenova,Aiym Amantaev,Bekzak Hu,Yin-Gang Feng,Bai-Li Zhunusbayeva,Zhazira
author_sort	Zhirnova,Irina
title	Pre-breeding for waxy proso millet by phenotyping and marker-assisted selection
title_short	Pre-breeding for waxy proso millet by phenotyping and marker-assisted selection
title_full	Pre-breeding for waxy proso millet by phenotyping and marker-assisted selection
title_fullStr	Pre-breeding for waxy proso millet by phenotyping and marker-assisted selection
title_full_unstemmed	Pre-breeding for waxy proso millet by phenotyping and marker-assisted selection
title_sort	pre-breeding for waxy proso millet by phenotyping and marker-assisted selection
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392021000400518
work_keys_str_mv	AT zhirnovairina prebreedingforwaxyprosomilletbyphenotypingandmarkerassistedselection AT rysbekovaaiman prebreedingforwaxyprosomilletbyphenotypingandmarkerassistedselection AT dyussibayevaelmira prebreedingforwaxyprosomilletbyphenotypingandmarkerassistedselection AT zhakenovaaiym prebreedingforwaxyprosomilletbyphenotypingandmarkerassistedselection AT amantaevbekzak prebreedingforwaxyprosomilletbyphenotypingandmarkerassistedselection AT huyingang prebreedingforwaxyprosomilletbyphenotypingandmarkerassistedselection AT fengbaili prebreedingforwaxyprosomilletbyphenotypingandmarkerassistedselection AT zhunusbayevazhazira prebreedingforwaxyprosomilletbyphenotypingandmarkerassistedselection
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Pre-breeding for waxy proso millet by phenotyping and marker-assisted selection

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