OPTIMAL PARAMETERS FORT In Vitro DEVELOPMENT OF THE HYDROCARBONOCLASTIC MICROORGANISM Proteus sp

In México, there are extensive areas polluted by oil spills. Currently, bioremediation technologies have been developed, using microorganisms to clean up oil sites. In this study, we evaluated the development of a hydrocarbonoclastic bacterial strain, using a completely randomized 3x3x4 factor arran...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hernández-Rivera,M.A, Ojeda-Morales,M.E, Martínez-Vázquez,J.G, Villegas-Cornelio,V.M, Córdova-Bautista,Y
Lenguaje:	English
Publicado:	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo 2011
Materias:	hydrocarbon degrading cells optimal growth Proteus sp
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162011000100003
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-95162011000100003
record_format	dspace
spelling	oai:scielo:S0718-951620110001000032011-03-25OPTIMAL PARAMETERS FORT In Vitro DEVELOPMENT OF THE HYDROCARBONOCLASTIC MICROORGANISM Proteus spHernández-Rivera,M.AOjeda-Morales,M.EMartínez-Vázquez,J.GVillegas-Cornelio,V.MCórdova-Bautista,Y hydrocarbon degrading cells optimal growth Proteus sp In México, there are extensive areas polluted by oil spills. Currently, bioremediation technologies have been developed, using microorganisms to clean up oil sites. In this study, we evaluated the development of a hydrocarbonoclastic bacterial strain, using a completely randomized 3x3x4 factor arrangement: three temperatures, three pH, and four nutrients. We collected samples of soil contaminated with 3.45 x 10(5) mg kg-1 (345,000 ppm) Total Petroleum Hydrocarbons (TPH), located at the Ejido José N. Rovirosa, Huimanguillo, Tabasco, México. The samples were grown in a culture medium Nutrient Agar (NA), obtaining a bacterial strain, which was characterized and classified as Proteus sp. The strain was grown in a combined carbon culture medium and then in a liquid mineral medium with crude oil as sole carbon source. Analysis of variance and mean test were performed, using the SPSS-11.0 statistical software. The microorganisms showed the highest population growth in the treatment N2=Triple17pH1=4.029°C with a value of 4.6 x 10(10) CFU mL-1. To reach, by bioaugmentation, the same development of Proteus sp in a conditioned soil would allow us to implement a potential bioremediation strategy for solving the problem of soils contaminated with hydrocarbons in the state of Tabasco in particular, and in Mexico in general.info:eu-repo/semantics/openAccessChilean Society of Soil Science / Sociedad Chilena de la Ciencia del SueloJournal of soil science and plant nutrition v.11 n.1 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162011000100003en10.4067/S0718-95162011000100003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	hydrocarbon degrading cells optimal growth Proteus sp
spellingShingle	hydrocarbon degrading cells optimal growth Proteus sp Hernández-Rivera,M.A Ojeda-Morales,M.E Martínez-Vázquez,J.G Villegas-Cornelio,V.M Córdova-Bautista,Y OPTIMAL PARAMETERS FORT In Vitro DEVELOPMENT OF THE HYDROCARBONOCLASTIC MICROORGANISM Proteus sp
description	In México, there are extensive areas polluted by oil spills. Currently, bioremediation technologies have been developed, using microorganisms to clean up oil sites. In this study, we evaluated the development of a hydrocarbonoclastic bacterial strain, using a completely randomized 3x3x4 factor arrangement: three temperatures, three pH, and four nutrients. We collected samples of soil contaminated with 3.45 x 10(5) mg kg-1 (345,000 ppm) Total Petroleum Hydrocarbons (TPH), located at the Ejido José N. Rovirosa, Huimanguillo, Tabasco, México. The samples were grown in a culture medium Nutrient Agar (NA), obtaining a bacterial strain, which was characterized and classified as Proteus sp. The strain was grown in a combined carbon culture medium and then in a liquid mineral medium with crude oil as sole carbon source. Analysis of variance and mean test were performed, using the SPSS-11.0 statistical software. The microorganisms showed the highest population growth in the treatment N2=Triple17pH1=4.029°C with a value of 4.6 x 10(10) CFU mL-1. To reach, by bioaugmentation, the same development of Proteus sp in a conditioned soil would allow us to implement a potential bioremediation strategy for solving the problem of soils contaminated with hydrocarbons in the state of Tabasco in particular, and in Mexico in general.
author	Hernández-Rivera,M.A Ojeda-Morales,M.E Martínez-Vázquez,J.G Villegas-Cornelio,V.M Córdova-Bautista,Y
author_facet	Hernández-Rivera,M.A Ojeda-Morales,M.E Martínez-Vázquez,J.G Villegas-Cornelio,V.M Córdova-Bautista,Y
author_sort	Hernández-Rivera,M.A
title	OPTIMAL PARAMETERS FORT In Vitro DEVELOPMENT OF THE HYDROCARBONOCLASTIC MICROORGANISM Proteus sp
title_short	OPTIMAL PARAMETERS FORT In Vitro DEVELOPMENT OF THE HYDROCARBONOCLASTIC MICROORGANISM Proteus sp
title_full	OPTIMAL PARAMETERS FORT In Vitro DEVELOPMENT OF THE HYDROCARBONOCLASTIC MICROORGANISM Proteus sp
title_fullStr	OPTIMAL PARAMETERS FORT In Vitro DEVELOPMENT OF THE HYDROCARBONOCLASTIC MICROORGANISM Proteus sp
title_full_unstemmed	OPTIMAL PARAMETERS FORT In Vitro DEVELOPMENT OF THE HYDROCARBONOCLASTIC MICROORGANISM Proteus sp
title_sort	optimal parameters fort in vitro development of the hydrocarbonoclastic microorganism proteus sp
publisher	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162011000100003
work_keys_str_mv	AT hernandezriverama optimalparametersfortinvitrodevelopmentofthehydrocarbonoclasticmicroorganismproteussp AT ojedamoralesme optimalparametersfortinvitrodevelopmentofthehydrocarbonoclasticmicroorganismproteussp AT martinezvazquezjg optimalparametersfortinvitrodevelopmentofthehydrocarbonoclasticmicroorganismproteussp AT villegascorneliovm optimalparametersfortinvitrodevelopmentofthehydrocarbonoclasticmicroorganismproteussp AT cordovabautistay optimalparametersfortinvitrodevelopmentofthehydrocarbonoclasticmicroorganismproteussp
_version_	1714206417537204224

OPTIMAL PARAMETERS FORT In Vitro DEVELOPMENT OF THE HYDROCARBONOCLASTIC MICROORGANISM Proteus sp

Ejemplares similares