MOLYBDATE TRANSPORT IN THE Bradyrhizobium japonicum - Glycine max L. SYMBIOSIS

For the molybdoenzymes synthesis as the nitrogenase, the molybdenum, in its most stable form, the molybdate, must be transported inside the cell. In Bradyrhizobium japonicum, the modABC genes code for a high-affinity ABC-type molybdate transporter. This work allowed to study the effect of inoculatio...

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Autores principales:	Tresierra-Ayala,Á, Delgado,M.J, Guzmán,R.A, Rengifo,A.L, Bedmar,E.J
Lenguaje:	English
Publicado:	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo 2011
Materias:	soybean Bradyrhizobium mod genes molybdate transport nitrogenase
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162011000200002
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spelling	oai:scielo:S0718-951620110002000022011-07-21MOLYBDATE TRANSPORT IN THE Bradyrhizobium japonicum - Glycine max L. SYMBIOSISTresierra-Ayala,ÁDelgado,M.JGuzmán,R.ARengifo,A.LBedmar,E.J soybean Bradyrhizobium mod genes molybdate transport nitrogenase For the molybdoenzymes synthesis as the nitrogenase, the molybdenum, in its most stable form, the molybdate, must be transported inside the cell. In Bradyrhizobium japonicum, the modABC genes code for a high-affinity ABC-type molybdate transporter. This work allowed to study the effect of inoculation of soybean plants with strains affected in the molybdate transport. modA and modB mutants, unable to grow in culture media under molybdate-deficient conditions, were used in our experiments. When soybean plants were inoculated with one of these strains and grown in a molybdate-deficient mineral solution did not affect the nodulation, but the nitrogen-fixing ability of the mod mutants was severely impaired. Addition of molybdate to the nutrient mineral solution used for plant growth fully restaured the wild-type phenotype, and the amount of molybdate required for supression of the mutant phenotype was dependent on sulfate concentration. Molybdate concentration required for the functioning of the mutant strains was greater when the medium was supplemented with high amounts of sulfate. Our results suggest the existence in B. japonicum, at least, three independent molybdate transport systems, a high-affinity transporter encoded by the modABC genes, a low affinity system corresponding to a sulfate transporter, and a third transporter that would be functional in the presence of high sulfate concentrations.info:eu-repo/semantics/openAccessChilean Society of Soil Science / Sociedad Chilena de la Ciencia del SueloJournal of soil science and plant nutrition v.11 n.2 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162011000200002en10.4067/S0718-95162011000200002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	soybean Bradyrhizobium mod genes molybdate transport nitrogenase
spellingShingle	soybean Bradyrhizobium mod genes molybdate transport nitrogenase Tresierra-Ayala,Á Delgado,M.J Guzmán,R.A Rengifo,A.L Bedmar,E.J MOLYBDATE TRANSPORT IN THE Bradyrhizobium japonicum - Glycine max L. SYMBIOSIS
description	For the molybdoenzymes synthesis as the nitrogenase, the molybdenum, in its most stable form, the molybdate, must be transported inside the cell. In Bradyrhizobium japonicum, the modABC genes code for a high-affinity ABC-type molybdate transporter. This work allowed to study the effect of inoculation of soybean plants with strains affected in the molybdate transport. modA and modB mutants, unable to grow in culture media under molybdate-deficient conditions, were used in our experiments. When soybean plants were inoculated with one of these strains and grown in a molybdate-deficient mineral solution did not affect the nodulation, but the nitrogen-fixing ability of the mod mutants was severely impaired. Addition of molybdate to the nutrient mineral solution used for plant growth fully restaured the wild-type phenotype, and the amount of molybdate required for supression of the mutant phenotype was dependent on sulfate concentration. Molybdate concentration required for the functioning of the mutant strains was greater when the medium was supplemented with high amounts of sulfate. Our results suggest the existence in B. japonicum, at least, three independent molybdate transport systems, a high-affinity transporter encoded by the modABC genes, a low affinity system corresponding to a sulfate transporter, and a third transporter that would be functional in the presence of high sulfate concentrations.
author	Tresierra-Ayala,Á Delgado,M.J Guzmán,R.A Rengifo,A.L Bedmar,E.J
author_facet	Tresierra-Ayala,Á Delgado,M.J Guzmán,R.A Rengifo,A.L Bedmar,E.J
author_sort	Tresierra-Ayala,Á
title	MOLYBDATE TRANSPORT IN THE Bradyrhizobium japonicum - Glycine max L. SYMBIOSIS
title_short	MOLYBDATE TRANSPORT IN THE Bradyrhizobium japonicum - Glycine max L. SYMBIOSIS
title_full	MOLYBDATE TRANSPORT IN THE Bradyrhizobium japonicum - Glycine max L. SYMBIOSIS
title_fullStr	MOLYBDATE TRANSPORT IN THE Bradyrhizobium japonicum - Glycine max L. SYMBIOSIS
title_full_unstemmed	MOLYBDATE TRANSPORT IN THE Bradyrhizobium japonicum - Glycine max L. SYMBIOSIS
title_sort	molybdate transport in the bradyrhizobium japonicum - glycine max l. symbiosis
publisher	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162011000200002
work_keys_str_mv	AT tresierraayalaa molybdatetransportinthebradyrhizobiumjaponicumglycinemaxlsymbiosis AT delgadomj molybdatetransportinthebradyrhizobiumjaponicumglycinemaxlsymbiosis AT guzmanra molybdatetransportinthebradyrhizobiumjaponicumglycinemaxlsymbiosis AT rengifoal molybdatetransportinthebradyrhizobiumjaponicumglycinemaxlsymbiosis AT bedmarej molybdatetransportinthebradyrhizobiumjaponicumglycinemaxlsymbiosis
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MOLYBDATE TRANSPORT IN THE Bradyrhizobium japonicum - Glycine max L. SYMBIOSIS

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