The effects of CaCO3 on adsorption, immobilization and activity of cellulase in a decarbonated soil

The interaction of organic molecules with mineral surfaces is a subject of interest in a variety of disciplines. The present study was done to elucidate some aspects of sorption and immobilization of cellulase on soil components by analysis of the sorption, desorption, immobilization and activity of...

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Autores principales:	Safari-Sinegani,A.A, Safari-Sinegani,M
Lenguaje:	English
Publicado:	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo 2011
Materias:	CaCO3 application Decarbonated soil Cellulase Sorption Immobilization Activity
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162011000300008
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spelling	oai:scielo:S0718-951620110003000082012-05-28The effects of CaCO3 on adsorption, immobilization and activity of cellulase in a decarbonated soilSafari-Sinegani,A.ASafari-Sinegani,M CaCO3 application Decarbonated soil Cellulase Sorption Immobilization Activity The interaction of organic molecules with mineral surfaces is a subject of interest in a variety of disciplines. The present study was done to elucidate some aspects of sorption and immobilization of cellulase on soil components by analysis of the sorption, desorption, immobilization and activity of cellulase on a decarbonated soil treated with different levels of CaCO3 (0, 2.5, 10 and 20 %). Applied concentrations of cellulase protein on Ca-homoionized soil suspensions were 0, 0.014, 0.028, 0.070, 0.140, 0.280, 0.701, 0.981 and 1.402 mg mL-1. After shaking for 1 h in sterile conditions, they were centrifuged and the amount of the cellulase protein remaining in solution was determined. The adsorbed cellulase protein was calculated. The immobilized and desorbed cellulase proteins were calculated after washing soil suspensions thrice with distilled water. Analysis of variance showed that the effects of the enzyme concentration, CaCO3 level and their interaction on cellulase protein adsorption and activity were statistically significant. The adsorption and immobilization capacities of the decarbonated soil increased by application of CaCO3. However, these effects of CaCO3 were only significant when high concentrations of cellulase protein were added to the soil. The desorption of cellulase protein from the decarbonated soil did not depend on the amount of cellulase adsorbed on the soil and the CaCO3 level in the soil. The immobilized cellulase activity, and particularly its specific activity, decreased considerably by increasing CaCO3 levels in the soil. This negative effect of CaCO3 on the cellulase specific activity in the decarbonated soil was significant even for low levels.info:eu-repo/semantics/openAccessChilean Society of Soil Science / Sociedad Chilena de la Ciencia del SueloJournal of soil science and plant nutrition v.11 n.3 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162011000300008en10.4067/S0718-95162011000300008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	CaCO3 application Decarbonated soil Cellulase Sorption Immobilization Activity
spellingShingle	CaCO3 application Decarbonated soil Cellulase Sorption Immobilization Activity Safari-Sinegani,A.A Safari-Sinegani,M The effects of CaCO3 on adsorption, immobilization and activity of cellulase in a decarbonated soil
description	The interaction of organic molecules with mineral surfaces is a subject of interest in a variety of disciplines. The present study was done to elucidate some aspects of sorption and immobilization of cellulase on soil components by analysis of the sorption, desorption, immobilization and activity of cellulase on a decarbonated soil treated with different levels of CaCO3 (0, 2.5, 10 and 20 %). Applied concentrations of cellulase protein on Ca-homoionized soil suspensions were 0, 0.014, 0.028, 0.070, 0.140, 0.280, 0.701, 0.981 and 1.402 mg mL-1. After shaking for 1 h in sterile conditions, they were centrifuged and the amount of the cellulase protein remaining in solution was determined. The adsorbed cellulase protein was calculated. The immobilized and desorbed cellulase proteins were calculated after washing soil suspensions thrice with distilled water. Analysis of variance showed that the effects of the enzyme concentration, CaCO3 level and their interaction on cellulase protein adsorption and activity were statistically significant. The adsorption and immobilization capacities of the decarbonated soil increased by application of CaCO3. However, these effects of CaCO3 were only significant when high concentrations of cellulase protein were added to the soil. The desorption of cellulase protein from the decarbonated soil did not depend on the amount of cellulase adsorbed on the soil and the CaCO3 level in the soil. The immobilized cellulase activity, and particularly its specific activity, decreased considerably by increasing CaCO3 levels in the soil. This negative effect of CaCO3 on the cellulase specific activity in the decarbonated soil was significant even for low levels.
author	Safari-Sinegani,A.A Safari-Sinegani,M
author_facet	Safari-Sinegani,A.A Safari-Sinegani,M
author_sort	Safari-Sinegani,A.A
title	The effects of CaCO3 on adsorption, immobilization and activity of cellulase in a decarbonated soil
title_short	The effects of CaCO3 on adsorption, immobilization and activity of cellulase in a decarbonated soil
title_full	The effects of CaCO3 on adsorption, immobilization and activity of cellulase in a decarbonated soil
title_fullStr	The effects of CaCO3 on adsorption, immobilization and activity of cellulase in a decarbonated soil
title_full_unstemmed	The effects of CaCO3 on adsorption, immobilization and activity of cellulase in a decarbonated soil
title_sort	effects of caco3 on adsorption, immobilization and activity of cellulase in a decarbonated soil
publisher	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162011000300008
work_keys_str_mv	AT safarisineganiaa theeffectsofcaco3onadsorptionimmobilizationandactivityofcellulaseinadecarbonatedsoil AT safarisineganim theeffectsofcaco3onadsorptionimmobilizationandactivityofcellulaseinadecarbonatedsoil AT safarisineganiaa effectsofcaco3onadsorptionimmobilizationandactivityofcellulaseinadecarbonatedsoil AT safarisineganim effectsofcaco3onadsorptionimmobilizationandactivityofcellulaseinadecarbonatedsoil
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The effects of CaCO3 on adsorption, immobilization and activity of cellulase in a decarbonated soil

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