Statistical optimization for improved indole-3-acetic acid (iaa) production by Pseudomonas aeruginosa and demonstration of enhanced plant growth promotion

The present study was undertaken to study the statistical optimization of medium components for improved Indole-3- acetic acid (IAA) production by Pseudomonas aeruginosa. Colorimetric analysis showed maximum IAA (132 ìg mL-1) in the medium supplemented with tryptophan (0.5 g L-1). The maximum produc...

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Autores principales:	Sasirekha,B, Shivakumar,S
Lenguaje:	English
Publicado:	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo 2012
Materias:	indole-3- acetic acid Plackett- Burman Design Pseudomonas aeruginosa GC- MS seed bacterization
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162012000400018
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spelling	oai:scielo:S0718-951620120004000182013-04-02Statistical optimization for improved indole-3-acetic acid (iaa) production by Pseudomonas aeruginosa and demonstration of enhanced plant growth promotionSasirekha,BShivakumar,S indole-3- acetic acid Plackett- Burman Design Pseudomonas aeruginosa GC- MS seed bacterization The present study was undertaken to study the statistical optimization of medium components for improved Indole-3- acetic acid (IAA) production by Pseudomonas aeruginosa. Colorimetric analysis showed maximum IAA (132 ìg mL-1) in the medium supplemented with tryptophan (0.5 g L-1). The maximum production was achieved after 96 h of incubation. Yeast extract, tryptophan and EDTA were identified as significant components influencing IAA production by Pseudomonas aeruginosa, using the Plackett- Burman method. The statistical optimization approach led to the production of 318 ìg mL-1 of IAA within 24 h of incubation. Statistical approach was found to be very effective in optimizing the medium components in a manageable number of experimental runs with overall 2.4 fold increase in IAA production. TLC and GC- MS analysis further confirmed the IAA production in the cell filtrates of the strain. GC-MS analysis and tryptophan side chain oxidase confirmed the existence of atleast 2 possible pathways for IAA by this strain. Inoculation of P. aeruginosa culture filtrate enhanced seed germination (82.4%) and increase in root length and shoot length (~ 2.6 and ~ 1.1 folds over the control) of cowpea seeds over the control treatment under pot culture conditions.info:eu-repo/semantics/openAccessChilean Society of Soil Science / Sociedad Chilena de la Ciencia del SueloJournal of soil science and plant nutrition v.12 n.4 20122012-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162012000400018en10.4067/S0718-95162012005000038
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	indole-3- acetic acid Plackett- Burman Design Pseudomonas aeruginosa GC- MS seed bacterization
spellingShingle	indole-3- acetic acid Plackett- Burman Design Pseudomonas aeruginosa GC- MS seed bacterization Sasirekha,B Shivakumar,S Statistical optimization for improved indole-3-acetic acid (iaa) production by Pseudomonas aeruginosa and demonstration of enhanced plant growth promotion
description	The present study was undertaken to study the statistical optimization of medium components for improved Indole-3- acetic acid (IAA) production by Pseudomonas aeruginosa. Colorimetric analysis showed maximum IAA (132 ìg mL-1) in the medium supplemented with tryptophan (0.5 g L-1). The maximum production was achieved after 96 h of incubation. Yeast extract, tryptophan and EDTA were identified as significant components influencing IAA production by Pseudomonas aeruginosa, using the Plackett- Burman method. The statistical optimization approach led to the production of 318 ìg mL-1 of IAA within 24 h of incubation. Statistical approach was found to be very effective in optimizing the medium components in a manageable number of experimental runs with overall 2.4 fold increase in IAA production. TLC and GC- MS analysis further confirmed the IAA production in the cell filtrates of the strain. GC-MS analysis and tryptophan side chain oxidase confirmed the existence of atleast 2 possible pathways for IAA by this strain. Inoculation of P. aeruginosa culture filtrate enhanced seed germination (82.4%) and increase in root length and shoot length (~ 2.6 and ~ 1.1 folds over the control) of cowpea seeds over the control treatment under pot culture conditions.
author	Sasirekha,B Shivakumar,S
author_facet	Sasirekha,B Shivakumar,S
author_sort	Sasirekha,B
title	Statistical optimization for improved indole-3-acetic acid (iaa) production by Pseudomonas aeruginosa and demonstration of enhanced plant growth promotion
title_short	Statistical optimization for improved indole-3-acetic acid (iaa) production by Pseudomonas aeruginosa and demonstration of enhanced plant growth promotion
title_full	Statistical optimization for improved indole-3-acetic acid (iaa) production by Pseudomonas aeruginosa and demonstration of enhanced plant growth promotion
title_fullStr	Statistical optimization for improved indole-3-acetic acid (iaa) production by Pseudomonas aeruginosa and demonstration of enhanced plant growth promotion
title_full_unstemmed	Statistical optimization for improved indole-3-acetic acid (iaa) production by Pseudomonas aeruginosa and demonstration of enhanced plant growth promotion
title_sort	statistical optimization for improved indole-3-acetic acid (iaa) production by pseudomonas aeruginosa and demonstration of enhanced plant growth promotion
publisher	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162012000400018
work_keys_str_mv	AT sasirekhab statisticaloptimizationforimprovedindole3aceticacidiaaproductionbypseudomonasaeruginosaanddemonstrationofenhancedplantgrowthpromotion AT shivakumars statisticaloptimizationforimprovedindole3aceticacidiaaproductionbypseudomonasaeruginosaanddemonstrationofenhancedplantgrowthpromotion
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Statistical optimization for improved indole-3-acetic acid (iaa) production by Pseudomonas aeruginosa and demonstration of enhanced plant growth promotion

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