Distribution of actinomycetes in different soil ecosystems and effect of media composition on extracellular phosphatase activity

Actinomycetes are widely distributed in different habitats and involved in important processes. Therefore, evaluation of their distribution is important in understanding their ecological role. Ninety seven samples were taken from different soil ecosystems (forest, pasture, rain-fed and irrigated cul...

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Autores principales:	Ghorbani-Nasrabadi,R, Greiner,R, Alikhani,H. A, Hamedi,J, Yakhchali,B
Lenguaje:	English
Publicado:	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo 2013
Materias:	Actinomycetes land use phosphatase phosphorus phytate phytate-degrading enzyme
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162013000100020
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spelling	oai:scielo:S0718-951620130001000202014-09-16Distribution of actinomycetes in different soil ecosystems and effect of media composition on extracellular phosphatase activityGhorbani-Nasrabadi,RGreiner,RAlikhani,H. AHamedi,JYakhchali,B Actinomycetes land use phosphatase phosphorus phytate phytate-degrading enzyme Actinomycetes are widely distributed in different habitats and involved in important processes. Therefore, evaluation of their distribution is important in understanding their ecological role. Ninety seven samples were taken from different soil ecosystems (forest, pasture, rain-fed and irrigated cultivated land) located in various climatic zones in the province of Golestan, Northeast Iran. The number of actinomycetes as well as pH value, organic carbon (OC) and soil salinity expressed as electrical conductivity (EC) showed significant differences in the soil ecosystems under investigation. The number of actinomycetes decreased from 2.86 * 10 6cfu g-1 in irrigated cultivated land to 7* 10(5) cfu g-1 in pasture (p<0.01). In pastures, the number of actinomycetes was negatively correlated with EC (r=-0.60, n=15, p<0.001) and pH (r=-0.59, n=15, p<0.001). In other soil ecosystems under investigation no significant correlations between soil pH, OC and EC and the number of actinomycetes could be found. In forest soils, the number of actinomycetes was significantly dependent (p<0.05) on climate. A significantly higher population was observed under semi-arid conditions compared to all other climatic zones (humid, sub-humid, Mediterranean). Because many soil microorganisms are able to transform different forms of insoluble organic and inorganic phosphorus into a soluble form suitable for plant uptake, the capacity of the isolated actinomycetes to secrete phosphatase activity was determined under laboratory conditions. A huge variation in the capacities to produce acid and alkaline phosphatases among the different isolates was observed. These enzyme activities and the capacities to hydrolyze phytate in the fermentation broth were significantly dependent on medium composition. In general, substitution of arginine and glycerol in the modified glycerol arginine medium (MGA) with other nitrogen and carbon sources resulted in a significant reduction of phytate dephosphorylation.info:eu-repo/semantics/openAccessChilean Society of Soil Science / Sociedad Chilena de la Ciencia del SueloJournal of soil science and plant nutrition v.13 n.1 20132013-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162013000100020en10.4067/S0718-95162013005000020
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Actinomycetes land use phosphatase phosphorus phytate phytate-degrading enzyme
spellingShingle	Actinomycetes land use phosphatase phosphorus phytate phytate-degrading enzyme Ghorbani-Nasrabadi,R Greiner,R Alikhani,H. A Hamedi,J Yakhchali,B Distribution of actinomycetes in different soil ecosystems and effect of media composition on extracellular phosphatase activity
description	Actinomycetes are widely distributed in different habitats and involved in important processes. Therefore, evaluation of their distribution is important in understanding their ecological role. Ninety seven samples were taken from different soil ecosystems (forest, pasture, rain-fed and irrigated cultivated land) located in various climatic zones in the province of Golestan, Northeast Iran. The number of actinomycetes as well as pH value, organic carbon (OC) and soil salinity expressed as electrical conductivity (EC) showed significant differences in the soil ecosystems under investigation. The number of actinomycetes decreased from 2.86 * 10 6cfu g-1 in irrigated cultivated land to 7* 10(5) cfu g-1 in pasture (p<0.01). In pastures, the number of actinomycetes was negatively correlated with EC (r=-0.60, n=15, p<0.001) and pH (r=-0.59, n=15, p<0.001). In other soil ecosystems under investigation no significant correlations between soil pH, OC and EC and the number of actinomycetes could be found. In forest soils, the number of actinomycetes was significantly dependent (p<0.05) on climate. A significantly higher population was observed under semi-arid conditions compared to all other climatic zones (humid, sub-humid, Mediterranean). Because many soil microorganisms are able to transform different forms of insoluble organic and inorganic phosphorus into a soluble form suitable for plant uptake, the capacity of the isolated actinomycetes to secrete phosphatase activity was determined under laboratory conditions. A huge variation in the capacities to produce acid and alkaline phosphatases among the different isolates was observed. These enzyme activities and the capacities to hydrolyze phytate in the fermentation broth were significantly dependent on medium composition. In general, substitution of arginine and glycerol in the modified glycerol arginine medium (MGA) with other nitrogen and carbon sources resulted in a significant reduction of phytate dephosphorylation.
author	Ghorbani-Nasrabadi,R Greiner,R Alikhani,H. A Hamedi,J Yakhchali,B
author_facet	Ghorbani-Nasrabadi,R Greiner,R Alikhani,H. A Hamedi,J Yakhchali,B
author_sort	Ghorbani-Nasrabadi,R
title	Distribution of actinomycetes in different soil ecosystems and effect of media composition on extracellular phosphatase activity
title_short	Distribution of actinomycetes in different soil ecosystems and effect of media composition on extracellular phosphatase activity
title_full	Distribution of actinomycetes in different soil ecosystems and effect of media composition on extracellular phosphatase activity
title_fullStr	Distribution of actinomycetes in different soil ecosystems and effect of media composition on extracellular phosphatase activity
title_full_unstemmed	Distribution of actinomycetes in different soil ecosystems and effect of media composition on extracellular phosphatase activity
title_sort	distribution of actinomycetes in different soil ecosystems and effect of media composition on extracellular phosphatase activity
publisher	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162013000100020
work_keys_str_mv	AT ghorbaninasrabadir distributionofactinomycetesindifferentsoilecosystemsandeffectofmediacompositiononextracellularphosphataseactivity AT greinerr distributionofactinomycetesindifferentsoilecosystemsandeffectofmediacompositiononextracellularphosphataseactivity AT alikhaniha distributionofactinomycetesindifferentsoilecosystemsandeffectofmediacompositiononextracellularphosphataseactivity AT hamedij distributionofactinomycetesindifferentsoilecosystemsandeffectofmediacompositiononextracellularphosphataseactivity AT yakhchalib distributionofactinomycetesindifferentsoilecosystemsandeffectofmediacompositiononextracellularphosphataseactivity
_version_	1714206452434862080

Distribution of actinomycetes in different soil ecosystems and effect of media composition on extracellular phosphatase activity

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