Effects of the nitrification inhibitor DMPP on soil bacterial community in a Cambisol in northeast China

A long-term experimental site was built to study effects of the nitrification inhibitor 3, 4-dimethylpyrazole phosphate (DMPP) on a bacterial population's diversity and activity in a Cambisol in northern China. Treatments included no fertilization (CK), application of urea alone (U), and applic...

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Autores principales:	Dong,X.X, Zhang,L.L, Wu,Z.J, Li,D.P, Shang,Z.C, Gong,P
Lenguaje:	English
Publicado:	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo 2013
Materias:	Nitrification inhibitor soil total bacteria ammonium-oxidizing bacteria ammonium-oxidizing archaea long-term experiment
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162013000300006
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spelling	oai:scielo:S0718-951620130003000062014-09-16Effects of the nitrification inhibitor DMPP on soil bacterial community in a Cambisol in northeast ChinaDong,X.XZhang,L.LWu,Z.JLi,D.PShang,Z.CGong,P Nitrification inhibitor soil total bacteria ammonium-oxidizing bacteria ammonium-oxidizing archaea long-term experiment A long-term experimental site was built to study effects of the nitrification inhibitor 3, 4-dimethylpyrazole phosphate (DMPP) on a bacterial population's diversity and activity in a Cambisol in northern China. Treatments included no fertilization (CK), application of urea alone (U), and application of urea plus DMPP (UD). The annual application rate for the urea was 180 kg N ha-1, and that of the DMPP was 1.8 kg ha-1. The diversity and composition of the overall soil bacterial community were analyzed using pyrosequencing, and the abundances of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) were analyzed using a real-time polymerase chain reaction (RT-PCR) assay. The dominant phyla were Proteobacteria, Acidobacteria, Actinobacteria, Bacteroidetes, Gemmatimonadetes, Chloroflexi, Firmicutes, and Planctomycetes in all of the samples. However, compared with treatment U, the relative abundance and identities of the dominant phyla that were increased in treatment UD were more similar to those of the CK treatment. DMPP significantly reduced the targeted ammonia oxidizing bacterial abundance, and the soil potential nitrification rate had a significant positive correlation with the amoA gene copy number of the AOB (r=0.685, n=9, p<0.05) but not of the AOA. The results suggested that long-term application of DMPP to this agricultural soil was relatively beneficial for both urea application and soil bacterial ecosystem reversion.info:eu-repo/semantics/openAccessChilean Society of Soil Science / Sociedad Chilena de la Ciencia del SueloJournal of soil science and plant nutrition v.13 n.3 20132013-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162013000300006en10.4067/S0718-95162013005000046
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Nitrification inhibitor soil total bacteria ammonium-oxidizing bacteria ammonium-oxidizing archaea long-term experiment
spellingShingle	Nitrification inhibitor soil total bacteria ammonium-oxidizing bacteria ammonium-oxidizing archaea long-term experiment Dong,X.X Zhang,L.L Wu,Z.J Li,D.P Shang,Z.C Gong,P Effects of the nitrification inhibitor DMPP on soil bacterial community in a Cambisol in northeast China
description	A long-term experimental site was built to study effects of the nitrification inhibitor 3, 4-dimethylpyrazole phosphate (DMPP) on a bacterial population's diversity and activity in a Cambisol in northern China. Treatments included no fertilization (CK), application of urea alone (U), and application of urea plus DMPP (UD). The annual application rate for the urea was 180 kg N ha-1, and that of the DMPP was 1.8 kg ha-1. The diversity and composition of the overall soil bacterial community were analyzed using pyrosequencing, and the abundances of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) were analyzed using a real-time polymerase chain reaction (RT-PCR) assay. The dominant phyla were Proteobacteria, Acidobacteria, Actinobacteria, Bacteroidetes, Gemmatimonadetes, Chloroflexi, Firmicutes, and Planctomycetes in all of the samples. However, compared with treatment U, the relative abundance and identities of the dominant phyla that were increased in treatment UD were more similar to those of the CK treatment. DMPP significantly reduced the targeted ammonia oxidizing bacterial abundance, and the soil potential nitrification rate had a significant positive correlation with the amoA gene copy number of the AOB (r=0.685, n=9, p<0.05) but not of the AOA. The results suggested that long-term application of DMPP to this agricultural soil was relatively beneficial for both urea application and soil bacterial ecosystem reversion.
author	Dong,X.X Zhang,L.L Wu,Z.J Li,D.P Shang,Z.C Gong,P
author_facet	Dong,X.X Zhang,L.L Wu,Z.J Li,D.P Shang,Z.C Gong,P
author_sort	Dong,X.X
title	Effects of the nitrification inhibitor DMPP on soil bacterial community in a Cambisol in northeast China
title_short	Effects of the nitrification inhibitor DMPP on soil bacterial community in a Cambisol in northeast China
title_full	Effects of the nitrification inhibitor DMPP on soil bacterial community in a Cambisol in northeast China
title_fullStr	Effects of the nitrification inhibitor DMPP on soil bacterial community in a Cambisol in northeast China
title_full_unstemmed	Effects of the nitrification inhibitor DMPP on soil bacterial community in a Cambisol in northeast China
title_sort	effects of the nitrification inhibitor dmpp on soil bacterial community in a cambisol in northeast china
publisher	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162013000300006
work_keys_str_mv	AT dongxx effectsofthenitrificationinhibitordmpponsoilbacterialcommunityinacambisolinnortheastchina AT zhangll effectsofthenitrificationinhibitordmpponsoilbacterialcommunityinacambisolinnortheastchina AT wuzj effectsofthenitrificationinhibitordmpponsoilbacterialcommunityinacambisolinnortheastchina AT lidp effectsofthenitrificationinhibitordmpponsoilbacterialcommunityinacambisolinnortheastchina AT shangzc effectsofthenitrificationinhibitordmpponsoilbacterialcommunityinacambisolinnortheastchina AT gongp effectsofthenitrificationinhibitordmpponsoilbacterialcommunityinacambisolinnortheastchina
_version_	1714206460275064832

Effects of the nitrification inhibitor DMPP on soil bacterial community in a Cambisol in northeast China

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