Responses of nitrogen metabolism to copper stress in Luffa cylindrica roots

Pot experiments were performed to investigate the responses of nitrogen metabolism to copper stress in Luffa cylindrica roots. Four treatments were used, in which varying copper concentrations (25, 50, 75 and 100 µM ) were added to MS medium. The fresh weights of the roots decreased gradually with t...

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Autores principales:	Zhang,L.-l, He,X.-j, Chen,M, An,R.-d, An,X. -l, Li,J
Lenguaje:	English
Publicado:	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo 2014
Materias:	Nitrogen metabolism copper stress nitrogen-assimilating enzymes
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162014000300009
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spelling	oai:scielo:S0718-951620140003000092015-03-31Responses of nitrogen metabolism to copper stress in Luffa cylindrica rootsZhang,L.-lHe,X.-jChen,MAn,R.-dAn,X. -lLi,J Nitrogen metabolism copper stress nitrogen-assimilating enzymes Pot experiments were performed to investigate the responses of nitrogen metabolism to copper stress in Luffa cylindrica roots. Four treatments were used, in which varying copper concentrations (25, 50, 75 and 100 µM ) were added to MS medium. The fresh weights of the roots decreased gradually with the increasing copper concentrations. At the lower concentrations, the ammonium and nitrite levels increased compared with the control, but the nitrate concentrations significantly decreased with 75 and 100 µM copper. Compared with the control, nitrate reductase activity levels gradually increased with increasing copper concentrations of up to 50 µM, and nitrite reductase activity levels significantly decreased. Copper stress led to variable increases in the activities of glutamine synthetase and glutamate synthase compared with the controls. The NADH- glutamate dehydrogenase (NADH-GDH) and NAD-glutamate dehydrogenase (NAD-GDH) activities were affected by the copper treatments, but that of NAD-GDH was significantly reduced by 100 µM copper. The activities of alanine aminotransferase (AlaAT) and aspartate aminotransferase (AspAT) showed varying changes following the copper treatments. The present results indicate that Luffa cylindrica shows altered activities of enzymes associated with nitrogen metabolism during copper stress, enabling it to monitor and adapt to changes in its N status and supply, thereby minimizing the harmful effects of the stress.info:eu-repo/semantics/openAccessChilean Society of Soil Science / Sociedad Chilena de la Ciencia del SueloJournal of soil science and plant nutrition v.14 n.3 20142014-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162014000300009en10.4067/S0718-95162014005000049
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Nitrogen metabolism copper stress nitrogen-assimilating enzymes
spellingShingle	Nitrogen metabolism copper stress nitrogen-assimilating enzymes Zhang,L.-l He,X.-j Chen,M An,R.-d An,X. -l Li,J Responses of nitrogen metabolism to copper stress in Luffa cylindrica roots
description	Pot experiments were performed to investigate the responses of nitrogen metabolism to copper stress in Luffa cylindrica roots. Four treatments were used, in which varying copper concentrations (25, 50, 75 and 100 µM ) were added to MS medium. The fresh weights of the roots decreased gradually with the increasing copper concentrations. At the lower concentrations, the ammonium and nitrite levels increased compared with the control, but the nitrate concentrations significantly decreased with 75 and 100 µM copper. Compared with the control, nitrate reductase activity levels gradually increased with increasing copper concentrations of up to 50 µM, and nitrite reductase activity levels significantly decreased. Copper stress led to variable increases in the activities of glutamine synthetase and glutamate synthase compared with the controls. The NADH- glutamate dehydrogenase (NADH-GDH) and NAD-glutamate dehydrogenase (NAD-GDH) activities were affected by the copper treatments, but that of NAD-GDH was significantly reduced by 100 µM copper. The activities of alanine aminotransferase (AlaAT) and aspartate aminotransferase (AspAT) showed varying changes following the copper treatments. The present results indicate that Luffa cylindrica shows altered activities of enzymes associated with nitrogen metabolism during copper stress, enabling it to monitor and adapt to changes in its N status and supply, thereby minimizing the harmful effects of the stress.
author	Zhang,L.-l He,X.-j Chen,M An,R.-d An,X. -l Li,J
author_facet	Zhang,L.-l He,X.-j Chen,M An,R.-d An,X. -l Li,J
author_sort	Zhang,L.-l
title	Responses of nitrogen metabolism to copper stress in Luffa cylindrica roots
title_short	Responses of nitrogen metabolism to copper stress in Luffa cylindrica roots
title_full	Responses of nitrogen metabolism to copper stress in Luffa cylindrica roots
title_fullStr	Responses of nitrogen metabolism to copper stress in Luffa cylindrica roots
title_full_unstemmed	Responses of nitrogen metabolism to copper stress in Luffa cylindrica roots
title_sort	responses of nitrogen metabolism to copper stress in luffa cylindrica roots
publisher	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162014000300009
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Responses of nitrogen metabolism to copper stress in Luffa cylindrica roots

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