Land use and seasonal effects on a Mediterranean soil bacterial community

To evaluate the effects of management practices and seasons on a soil bacterial community and the composition of ammonia-oxidizing bacteria (AOB), molecular screenings were compared among Mediterranean (Sardinia) soils with different plant covers and different agricultural practices, namely cork oak...

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Autores principales:	Francioli,D, Ascher,J, Ceccherini,M.T, Pietramellara,G
Lenguaje:	English
Publicado:	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo 2014
Materias:	Microbial biomass PCR-DGGE land management seasonal shifts ammonia oxidizing bacteria pooled soil samples
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162014000300017
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spelling	oai:scielo:S0718-951620140003000172015-03-31Land use and seasonal effects on a Mediterranean soil bacterial communityFrancioli,DAscher,JCeccherini,M.TPietramellara,G Microbial biomass PCR-DGGE land management seasonal shifts ammonia oxidizing bacteria pooled soil samples To evaluate the effects of management practices and seasons on a soil bacterial community and the composition of ammonia-oxidizing bacteria (AOB), molecular screenings were compared among Mediterranean (Sardinia) soils with different plant covers and different agricultural practices, namely cork oak forest, tilled/non-tilled vineyard, hay crop and pasture. We compared the fingerprints from both independent replicates and pooled samples to ascertain the best approach for studying the environmental effects on bacterial composition. The soil microbial biomass, which was estimated from the amounts of extracted soil dsDNA, was 2 to 3 folds higher in the spring than in the autumn; in the spring, it was negatively correlated with the intensity of land use. A 16S rDNA DGGE experiment confirmed that both the land use and season markedly affect the composition of the soil bacterial community. Tilled vineyard soil exhibited the lowest similarities in community structures, suggesting that tillage induced the most marked disturbance among the tested land management methods. Distinct AOB populations were found for each type of land use; among these types, the cork oak forest proved to be a protective habitat for AOB against environmental changes. Our results suggest that the comparative community level and group-specific fingerprinting enabled an accurate evaluation of multiple factors in soil bacterial structures when performed with both independent and pooled replicates.info:eu-repo/semantics/openAccessChilean Society of Soil Science / Sociedad Chilena de la Ciencia del SueloJournal of soil science and plant nutrition v.14 n.3 20142014-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162014000300017en10.4067/S0718-95162014005000057
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Microbial biomass PCR-DGGE land management seasonal shifts ammonia oxidizing bacteria pooled soil samples
spellingShingle	Microbial biomass PCR-DGGE land management seasonal shifts ammonia oxidizing bacteria pooled soil samples Francioli,D Ascher,J Ceccherini,M.T Pietramellara,G Land use and seasonal effects on a Mediterranean soil bacterial community
description	To evaluate the effects of management practices and seasons on a soil bacterial community and the composition of ammonia-oxidizing bacteria (AOB), molecular screenings were compared among Mediterranean (Sardinia) soils with different plant covers and different agricultural practices, namely cork oak forest, tilled/non-tilled vineyard, hay crop and pasture. We compared the fingerprints from both independent replicates and pooled samples to ascertain the best approach for studying the environmental effects on bacterial composition. The soil microbial biomass, which was estimated from the amounts of extracted soil dsDNA, was 2 to 3 folds higher in the spring than in the autumn; in the spring, it was negatively correlated with the intensity of land use. A 16S rDNA DGGE experiment confirmed that both the land use and season markedly affect the composition of the soil bacterial community. Tilled vineyard soil exhibited the lowest similarities in community structures, suggesting that tillage induced the most marked disturbance among the tested land management methods. Distinct AOB populations were found for each type of land use; among these types, the cork oak forest proved to be a protective habitat for AOB against environmental changes. Our results suggest that the comparative community level and group-specific fingerprinting enabled an accurate evaluation of multiple factors in soil bacterial structures when performed with both independent and pooled replicates.
author	Francioli,D Ascher,J Ceccherini,M.T Pietramellara,G
author_facet	Francioli,D Ascher,J Ceccherini,M.T Pietramellara,G
author_sort	Francioli,D
title	Land use and seasonal effects on a Mediterranean soil bacterial community
title_short	Land use and seasonal effects on a Mediterranean soil bacterial community
title_full	Land use and seasonal effects on a Mediterranean soil bacterial community
title_fullStr	Land use and seasonal effects on a Mediterranean soil bacterial community
title_full_unstemmed	Land use and seasonal effects on a Mediterranean soil bacterial community
title_sort	land use and seasonal effects on a mediterranean soil bacterial community
publisher	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162014000300017
work_keys_str_mv	AT franciolid landuseandseasonaleffectsonamediterraneansoilbacterialcommunity AT ascherj landuseandseasonaleffectsonamediterraneansoilbacterialcommunity AT ceccherinimt landuseandseasonaleffectsonamediterraneansoilbacterialcommunity AT pietramellarag landuseandseasonaleffectsonamediterraneansoilbacterialcommunity
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Land use and seasonal effects on a Mediterranean soil bacterial community

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