Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide
Combined microbiological and molecular test (BiologEcoplateTM, denaturing gradient gel electrophoresis (DGGE) and Real Time PCR (qPCR)) were carried out to evaluate the impact of repeated diazinon (DZN) applications at high concentration (40 mg kg-1) on microbial communities in a microcosm simulatin...
Guardado en:
| Autores principales: | , , , , , , |
|---|---|
| Lenguaje: | English |
| Publicado: |
Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo
2014
|
| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162014000400009 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:scielo:S0718-95162014000400009 |
|---|---|
| record_format |
dspace |
| spelling |
oai:scielo:S0718-951620140004000092015-01-20Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticideTortella,G.RSalgado,ECuozzo,S.AMella-Herrera,R.AParra,LDiez,M.CRubilar,O CLFP biopurification system microbial communities diazinon Combined microbiological and molecular test (BiologEcoplateTM, denaturing gradient gel electrophoresis (DGGE) and Real Time PCR (qPCR)) were carried out to evaluate the impact of repeated diazinon (DZN) applications at high concentration (40 mg kg-1) on microbial communities in a microcosm simulating the organic matrix (straw (50%): peat (25%): soil (25%) vv-1) of a pesticide biopurification system (PBS). Pesticide dissipation was also evaluated. After three successive exposures with DZN the dissipation efficiency was high; DZN dissipation clearly accelerated in the organic matrix, achieving 87%, 93% and 96% respectively after the three applications. The results obtained with BiologEcoplateTM showed that the physiological profiles of the community were not affected by the addition of DZN. On the other hand, molecular assays (DGGE and qPCR) demonstrated that the microbial structure (bacteria and fungi) remained relatively stable over time with high DZN doses compared to control. The results of the present study clearly demonstrate the high dissipation capacity of this biomixture and highlight the microbiological robustness of this biological system.info:eu-repo/semantics/openAccessChilean Society of Soil Science / Sociedad Chilena de la Ciencia del SueloJournal of soil science and plant nutrition v.14 n.4 20142014-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162014000400009en10.4067/S0718-95162014005000069 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
CLFP biopurification system microbial communities diazinon |
| spellingShingle |
CLFP biopurification system microbial communities diazinon Tortella,G.R Salgado,E Cuozzo,S.A Mella-Herrera,R.A Parra,L Diez,M.C Rubilar,O Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide |
| description |
Combined microbiological and molecular test (BiologEcoplateTM, denaturing gradient gel electrophoresis (DGGE) and Real Time PCR (qPCR)) were carried out to evaluate the impact of repeated diazinon (DZN) applications at high concentration (40 mg kg-1) on microbial communities in a microcosm simulating the organic matrix (straw (50%): peat (25%): soil (25%) vv-1) of a pesticide biopurification system (PBS). Pesticide dissipation was also evaluated. After three successive exposures with DZN the dissipation efficiency was high; DZN dissipation clearly accelerated in the organic matrix, achieving 87%, 93% and 96% respectively after the three applications. The results obtained with BiologEcoplateTM showed that the physiological profiles of the community were not affected by the addition of DZN. On the other hand, molecular assays (DGGE and qPCR) demonstrated that the microbial structure (bacteria and fungi) remained relatively stable over time with high DZN doses compared to control. The results of the present study clearly demonstrate the high dissipation capacity of this biomixture and highlight the microbiological robustness of this biological system. |
| author |
Tortella,G.R Salgado,E Cuozzo,S.A Mella-Herrera,R.A Parra,L Diez,M.C Rubilar,O |
| author_facet |
Tortella,G.R Salgado,E Cuozzo,S.A Mella-Herrera,R.A Parra,L Diez,M.C Rubilar,O |
| author_sort |
Tortella,G.R |
| title |
Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide |
| title_short |
Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide |
| title_full |
Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide |
| title_fullStr |
Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide |
| title_full_unstemmed |
Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide |
| title_sort |
combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide |
| publisher |
Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo |
| publishDate |
2014 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162014000400009 |
| work_keys_str_mv |
AT tortellagr combinedmicrobiologicaltesttoassesschangesinanorganicmatrixusedtoavoidagriculturalsoilcontaminationexposedtoaninsecticide AT salgadoe combinedmicrobiologicaltesttoassesschangesinanorganicmatrixusedtoavoidagriculturalsoilcontaminationexposedtoaninsecticide AT cuozzosa combinedmicrobiologicaltesttoassesschangesinanorganicmatrixusedtoavoidagriculturalsoilcontaminationexposedtoaninsecticide AT mellaherrerara combinedmicrobiologicaltesttoassesschangesinanorganicmatrixusedtoavoidagriculturalsoilcontaminationexposedtoaninsecticide AT parral combinedmicrobiologicaltesttoassesschangesinanorganicmatrixusedtoavoidagriculturalsoilcontaminationexposedtoaninsecticide AT diezmc combinedmicrobiologicaltesttoassesschangesinanorganicmatrixusedtoavoidagriculturalsoilcontaminationexposedtoaninsecticide AT rubilaro combinedmicrobiologicaltesttoassesschangesinanorganicmatrixusedtoavoidagriculturalsoilcontaminationexposedtoaninsecticide |
| _version_ |
1714206491065450496 |