Effect of glucose, root exudates and N forms in mycorrhizal symbiosis using Rhizophagus intraradices

We investigated the effects of glucose and root exudates in combination with different nitrogen (N) forms on the spore germination rate and hyphal growth of the arbuscular mycorrhizal (AM) fungus Rhizophagus intraradices. Spores were cultured for 20 days at 26 ºC in agar medium containing either glu...

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Autores principales:	Wang,Y, Tang,S, Jin,H
Lenguaje:	English
Publicado:	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo 2015
Materias:	Arbuscular mycorrhiza hyphal length nitrogen sources root exudate spore germination
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162015000300014
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spelling	oai:scielo:S0718-951620150003000142015-10-14Effect of glucose, root exudates and N forms in mycorrhizal symbiosis using Rhizophagus intraradicesWang,YTang,SJin,H Arbuscular mycorrhiza hyphal length nitrogen sources root exudate spore germination We investigated the effects of glucose and root exudates in combination with different nitrogen (N) forms on the spore germination rate and hyphal growth of the arbuscular mycorrhizal (AM) fungus Rhizophagus intraradices. Spores were cultured for 20 days at 26 ºC in agar medium containing either glucose or root exudates at different concentrations. Nitrogen was supplied in the form of 4 mM NaNO3, 4 mM NH4Cl, 4 mM NH4NO3 or 2 mM urea. Three different controls without added N were used: deionized water, glucose, and root exudates. After 20 days, Glucose concentrations of 10, 25, 40 and 50 g L-1 inhibited the spore germination; however, when the concentration of glucose was 5 g L-1, the mean hyphal length was longer than that in the control. Glucose plus different forms of N sources also has no positive effect on the germination as well as hyphal growth. The addition of root exudates significantly improved both the spore germination rate and hyphal lengths in all four media (root exudates +4 mmol NaNO3, root exudates +4 mmol NH4Cl, root exudates +4 mmol NH4NO3 and root exudates +2 mmol urea). After incubation for 20 days, the highest spore germination rates (80.33%) were detected in the root exudate + urea medium, while that in the root exudate + NaNO3 medium was lower than in the control. In all these four media, hyphal growth was initially rapid and slowed in the later stages of incubation. Finally, the greatest hyphal length (15. 15 mmspores-1 was observed in the root exudate + NH4NO3 medium, while the lowest was observed in the exudate + NaNO3 medium.info:eu-repo/semantics/openAccessChilean Society of Soil Science / Sociedad Chilena de la Ciencia del SueloJournal of soil science and plant nutrition v.15 n.3 20152015-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162015000300014en10.4067/S0718-95162015005000049
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Arbuscular mycorrhiza hyphal length nitrogen sources root exudate spore germination
spellingShingle	Arbuscular mycorrhiza hyphal length nitrogen sources root exudate spore germination Wang,Y Tang,S Jin,H Effect of glucose, root exudates and N forms in mycorrhizal symbiosis using Rhizophagus intraradices
description	We investigated the effects of glucose and root exudates in combination with different nitrogen (N) forms on the spore germination rate and hyphal growth of the arbuscular mycorrhizal (AM) fungus Rhizophagus intraradices. Spores were cultured for 20 days at 26 ºC in agar medium containing either glucose or root exudates at different concentrations. Nitrogen was supplied in the form of 4 mM NaNO3, 4 mM NH4Cl, 4 mM NH4NO3 or 2 mM urea. Three different controls without added N were used: deionized water, glucose, and root exudates. After 20 days, Glucose concentrations of 10, 25, 40 and 50 g L-1 inhibited the spore germination; however, when the concentration of glucose was 5 g L-1, the mean hyphal length was longer than that in the control. Glucose plus different forms of N sources also has no positive effect on the germination as well as hyphal growth. The addition of root exudates significantly improved both the spore germination rate and hyphal lengths in all four media (root exudates +4 mmol NaNO3, root exudates +4 mmol NH4Cl, root exudates +4 mmol NH4NO3 and root exudates +2 mmol urea). After incubation for 20 days, the highest spore germination rates (80.33%) were detected in the root exudate + urea medium, while that in the root exudate + NaNO3 medium was lower than in the control. In all these four media, hyphal growth was initially rapid and slowed in the later stages of incubation. Finally, the greatest hyphal length (15. 15 mmspores-1 was observed in the root exudate + NH4NO3 medium, while the lowest was observed in the exudate + NaNO3 medium.
author	Wang,Y Tang,S Jin,H
author_facet	Wang,Y Tang,S Jin,H
author_sort	Wang,Y
title	Effect of glucose, root exudates and N forms in mycorrhizal symbiosis using Rhizophagus intraradices
title_short	Effect of glucose, root exudates and N forms in mycorrhizal symbiosis using Rhizophagus intraradices
title_full	Effect of glucose, root exudates and N forms in mycorrhizal symbiosis using Rhizophagus intraradices
title_fullStr	Effect of glucose, root exudates and N forms in mycorrhizal symbiosis using Rhizophagus intraradices
title_full_unstemmed	Effect of glucose, root exudates and N forms in mycorrhizal symbiosis using Rhizophagus intraradices
title_sort	effect of glucose, root exudates and n forms in mycorrhizal symbiosis using rhizophagus intraradices
publisher	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162015000300014
work_keys_str_mv	AT wangy effectofglucoserootexudatesandnformsinmycorrhizalsymbiosisusingrhizophagusintraradices AT tangs effectofglucoserootexudatesandnformsinmycorrhizalsymbiosisusingrhizophagusintraradices AT jinh effectofglucoserootexudatesandnformsinmycorrhizalsymbiosisusingrhizophagusintraradices
_version_	1714206512540286976

Effect of glucose, root exudates and N forms in mycorrhizal symbiosis using Rhizophagus intraradices

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