Optimization of phosphate solubilization by Aspergillus niger using plackett-burman and response surface methodology

Phosphorus is one of the major components required for the metabolic activities and for the growth of any organism. Many soil organisms are known to solubilize inorganic phosphates. Aspergillus niger isolated from the soil showed extensive solubilization of Tri-calcium phosphate.It was observed that...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autor principal:	Padmavathi,T
Lenguaje:	English
Publicado:	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo 2015
Materias:	Phosphate solubilizing fungi Plackett-Burman response surface methodology organic acids
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162015000300018
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-95162015000300018
record_format	dspace
spelling	oai:scielo:S0718-951620150003000182015-10-14Optimization of phosphate solubilization by Aspergillus niger using plackett-burman and response surface methodologyPadmavathi,T Phosphate solubilizing fungi Plackett-Burman response surface methodology organic acids Phosphorus is one of the major components required for the metabolic activities and for the growth of any organism. Many soil organisms are known to solubilize inorganic phosphates. Aspergillus niger isolated from the soil showed extensive solubilization of Tri-calcium phosphate.It was observed that the solubilization was due to drop in pH.Acidification was due to production of organic acids by the fungi. The effect of different medium components on the solubilization of phosphate was determined using the Packett-Burman design. It was observed that glucose and ammonium sulphate had significant effect on phosphate solubilization.Considering the Plackett-Burman results, the response surface methodology was used for optimization of these medium components along with tri-calcium phosphate on P-solublization. The analysis from RSM revealed that the optimum values for the tested variables were glucose - 2g/50ml, ammonium sulphate -0.2g/50ml and tri-calcium phosphate - 1g/50ml. Phosphate solubilization of 3.64 mg/ml was observed as comparison to original level of 1.88mg/ml, which was a 1.93-fold increase was obtained. From the HPLC analysis it was observed that oxalic acid and lactic acid were the major acids responsible for enhancing the P solubilization.info:eu-repo/semantics/openAccessChilean Society of Soil Science / Sociedad Chilena de la Ciencia del SueloJournal of soil science and plant nutrition v.15 n.3 20152015-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162015000300018en10.4067/S0718-95162015005000053
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Phosphate solubilizing fungi Plackett-Burman response surface methodology organic acids
spellingShingle	Phosphate solubilizing fungi Plackett-Burman response surface methodology organic acids Padmavathi,T Optimization of phosphate solubilization by Aspergillus niger using plackett-burman and response surface methodology
description	Phosphorus is one of the major components required for the metabolic activities and for the growth of any organism. Many soil organisms are known to solubilize inorganic phosphates. Aspergillus niger isolated from the soil showed extensive solubilization of Tri-calcium phosphate.It was observed that the solubilization was due to drop in pH.Acidification was due to production of organic acids by the fungi. The effect of different medium components on the solubilization of phosphate was determined using the Packett-Burman design. It was observed that glucose and ammonium sulphate had significant effect on phosphate solubilization.Considering the Plackett-Burman results, the response surface methodology was used for optimization of these medium components along with tri-calcium phosphate on P-solublization. The analysis from RSM revealed that the optimum values for the tested variables were glucose - 2g/50ml, ammonium sulphate -0.2g/50ml and tri-calcium phosphate - 1g/50ml. Phosphate solubilization of 3.64 mg/ml was observed as comparison to original level of 1.88mg/ml, which was a 1.93-fold increase was obtained. From the HPLC analysis it was observed that oxalic acid and lactic acid were the major acids responsible for enhancing the P solubilization.
author	Padmavathi,T
author_facet	Padmavathi,T
author_sort	Padmavathi,T
title	Optimization of phosphate solubilization by Aspergillus niger using plackett-burman and response surface methodology
title_short	Optimization of phosphate solubilization by Aspergillus niger using plackett-burman and response surface methodology
title_full	Optimization of phosphate solubilization by Aspergillus niger using plackett-burman and response surface methodology
title_fullStr	Optimization of phosphate solubilization by Aspergillus niger using plackett-burman and response surface methodology
title_full_unstemmed	Optimization of phosphate solubilization by Aspergillus niger using plackett-burman and response surface methodology
title_sort	optimization of phosphate solubilization by aspergillus niger using plackett-burman and response surface methodology
publisher	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162015000300018
work_keys_str_mv	AT padmavathit optimizationofphosphatesolubilizationbyaspergillusnigerusingplackettburmanandresponsesurfacemethodology
_version_	1714206513210327040

Optimization of phosphate solubilization by Aspergillus niger using plackett-burman and response surface methodology

Ejemplares similares