Determination of the fluorescence spectrum of Botrytis cinerea Pers: Fr. isolated from highbush blueberry (Vaccinium corymbosum L.)

The prompt diagnosis of an attack by phytopathogens associated with the deterioration of the quality and condition of highbush blueberry is of great interest, particularly when dealing with Botrytis cinerea in pre- and postharvest stages. Real-time technologies that complement traditional diagnostic...

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Autores principales:	Cerna Larenas,M, Guzmán Estrada,R, Guerrero Contreras,J, Meriño Gergichevich,C
Lenguaje:	English
Publicado:	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo 2015
Materias:	fluorescence spectrum highbush blueberry
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162015000400010
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spelling	oai:scielo:S0718-951620150004000102016-01-14Determination of the fluorescence spectrum of Botrytis cinerea Pers: Fr. isolated from highbush blueberry (Vaccinium corymbosum L.)Cerna Larenas,MGuzmán Estrada,RGuerrero Contreras,JMeriño Gergichevich,C fluorescence spectrum highbush blueberry The prompt diagnosis of an attack by phytopathogens associated with the deterioration of the quality and condition of highbush blueberry is of great interest, particularly when dealing with Botrytis cinerea in pre- and postharvest stages. Real-time technologies that complement traditional diagnostic methods during the processing, storage and transport of fruit are advisable. The aim of this study was to determine the fluorescence spectrum of the B. cinerea mycelium in culture medium as well as its temporal variation. Two evaluations were conducted daily for 18 days using a fluorimeter with a 100 mW, 405 nm laser light, which excited the mycelium of each of five isolates of B. cinerea in two fixed positions. The fluorescence emission of the malt agar culture medium and the emission obtained from the fungus Trichothecium roseum were quantified for comparative reference. It was found that B. cinerea fluoresces mainly in the red-infrared area of the spectrum and T. roseum does so with greater intensity in the green-red segment. The energy of the fluorescence spectrum did not vary significantly according to the development of the B. cinerea mycelium in artificial culture medium.info:eu-repo/semantics/openAccessChilean Society of Soil Science / Sociedad Chilena de la Ciencia del SueloJournal of soil science and plant nutrition v.15 n.4 20152015-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162015000400010en10.4067/S0718-95162015005000065
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	fluorescence spectrum highbush blueberry
spellingShingle	fluorescence spectrum highbush blueberry Cerna Larenas,M Guzmán Estrada,R Guerrero Contreras,J Meriño Gergichevich,C Determination of the fluorescence spectrum of Botrytis cinerea Pers: Fr. isolated from highbush blueberry (Vaccinium corymbosum L.)
description	The prompt diagnosis of an attack by phytopathogens associated with the deterioration of the quality and condition of highbush blueberry is of great interest, particularly when dealing with Botrytis cinerea in pre- and postharvest stages. Real-time technologies that complement traditional diagnostic methods during the processing, storage and transport of fruit are advisable. The aim of this study was to determine the fluorescence spectrum of the B. cinerea mycelium in culture medium as well as its temporal variation. Two evaluations were conducted daily for 18 days using a fluorimeter with a 100 mW, 405 nm laser light, which excited the mycelium of each of five isolates of B. cinerea in two fixed positions. The fluorescence emission of the malt agar culture medium and the emission obtained from the fungus Trichothecium roseum were quantified for comparative reference. It was found that B. cinerea fluoresces mainly in the red-infrared area of the spectrum and T. roseum does so with greater intensity in the green-red segment. The energy of the fluorescence spectrum did not vary significantly according to the development of the B. cinerea mycelium in artificial culture medium.
author	Cerna Larenas,M Guzmán Estrada,R Guerrero Contreras,J Meriño Gergichevich,C
author_facet	Cerna Larenas,M Guzmán Estrada,R Guerrero Contreras,J Meriño Gergichevich,C
author_sort	Cerna Larenas,M
title	Determination of the fluorescence spectrum of Botrytis cinerea Pers: Fr. isolated from highbush blueberry (Vaccinium corymbosum L.)
title_short	Determination of the fluorescence spectrum of Botrytis cinerea Pers: Fr. isolated from highbush blueberry (Vaccinium corymbosum L.)
title_full	Determination of the fluorescence spectrum of Botrytis cinerea Pers: Fr. isolated from highbush blueberry (Vaccinium corymbosum L.)
title_fullStr	Determination of the fluorescence spectrum of Botrytis cinerea Pers: Fr. isolated from highbush blueberry (Vaccinium corymbosum L.)
title_full_unstemmed	Determination of the fluorescence spectrum of Botrytis cinerea Pers: Fr. isolated from highbush blueberry (Vaccinium corymbosum L.)
title_sort	determination of the fluorescence spectrum of botrytis cinerea pers: fr. isolated from highbush blueberry (vaccinium corymbosum l.)
publisher	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162015000400010
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Determination of the fluorescence spectrum of Botrytis cinerea Pers: Fr. isolated from highbush blueberry (Vaccinium corymbosum L.)

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