Phytase-producing Bacillus sp. inoculation increases phosphorus availability in cattle manure

Organic wastes rich in phosphorus (P) are considered an alternative to decrease the dependence on chemical P fertilization in crops and pastures. Microbial inoculants are being studied as a tool to increase plant P availability in organic wastes. In this study, we explore the effect of inoculation w...

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Autores principales:	Menezes-Blackburn,D, Inostroza,N.G, Gianfreda,L, Greiner,R, Mora,M.L, Jorquera,M.A
Lenguaje:	English
Publicado:	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo 2016
Materias:	Bacillus cattle manure phytate phytase-producing bacteria phosphorus
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162016000100016
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spelling	oai:scielo:S0718-951620160001000162016-05-05Phytase-producing Bacillus sp. inoculation increases phosphorus availability in cattle manureMenezes-Blackburn,DInostroza,N.GGianfreda,LGreiner,RMora,M.LJorquera,M.A Bacillus cattle manure phytate phytase-producing bacteria phosphorus Organic wastes rich in phosphorus (P) are considered an alternative to decrease the dependence on chemical P fertilization in crops and pastures. Microbial inoculants are being studied as a tool to increase plant P availability in organic wastes. In this study, we explore the effect of inoculation with Bacillus sp. MQH-19 (a native phytase-producing bacterium) on the release of inorganic phosphorus (Pi) in cattle manure with low available P but a high total P content. Bacteria inoculation resulted in a higher release of Pi (8% in NaHCÜ3 and 13% in NaOH-EDTA extracts) compared with that of uninoculated manure (0.7% in NaHCÜ3 and 0.1% in NaOH-EDTA extracts). However, a greater amount of Pi was released in inoculated manure supplemented with phytate (47% in NaHCÜ3 and 117% in NaOH-EDTA extracts) compared with that of uninoculated manure supplemented with phytate (30% in NaHCÜ3 and 15% in NaOH-EDTA extracts). In addition, the use of denaturing gradient gel electrophoresis (DGGE) and quantitative PCR (qPCR) revealed that the bacterial community structure in manure was affected by inoculation and that the prevalence of Bacillus sp. MQH-19 decreased during incubation (6 days). This study demonstrates that Pi availability in cattle manure can be increased by phytase-producing bacteria inoculation. Phytase-producing bacteria inoculation might represent an attractive strategy to increase P availability in agricultural wastes, which are used as organic fertilizers in crops and pastures.info:eu-repo/semantics/openAccessChilean Society of Soil Science / Sociedad Chilena de la Ciencia del SueloJournal of soil science and plant nutrition v.16 n.1 20162016-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162016000100016en10.4067/S0718-95162016005000016
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Bacillus cattle manure phytate phytase-producing bacteria phosphorus
spellingShingle	Bacillus cattle manure phytate phytase-producing bacteria phosphorus Menezes-Blackburn,D Inostroza,N.G Gianfreda,L Greiner,R Mora,M.L Jorquera,M.A Phytase-producing Bacillus sp. inoculation increases phosphorus availability in cattle manure
description	Organic wastes rich in phosphorus (P) are considered an alternative to decrease the dependence on chemical P fertilization in crops and pastures. Microbial inoculants are being studied as a tool to increase plant P availability in organic wastes. In this study, we explore the effect of inoculation with Bacillus sp. MQH-19 (a native phytase-producing bacterium) on the release of inorganic phosphorus (Pi) in cattle manure with low available P but a high total P content. Bacteria inoculation resulted in a higher release of Pi (8% in NaHCÜ3 and 13% in NaOH-EDTA extracts) compared with that of uninoculated manure (0.7% in NaHCÜ3 and 0.1% in NaOH-EDTA extracts). However, a greater amount of Pi was released in inoculated manure supplemented with phytate (47% in NaHCÜ3 and 117% in NaOH-EDTA extracts) compared with that of uninoculated manure supplemented with phytate (30% in NaHCÜ3 and 15% in NaOH-EDTA extracts). In addition, the use of denaturing gradient gel electrophoresis (DGGE) and quantitative PCR (qPCR) revealed that the bacterial community structure in manure was affected by inoculation and that the prevalence of Bacillus sp. MQH-19 decreased during incubation (6 days). This study demonstrates that Pi availability in cattle manure can be increased by phytase-producing bacteria inoculation. Phytase-producing bacteria inoculation might represent an attractive strategy to increase P availability in agricultural wastes, which are used as organic fertilizers in crops and pastures.
author	Menezes-Blackburn,D Inostroza,N.G Gianfreda,L Greiner,R Mora,M.L Jorquera,M.A
author_facet	Menezes-Blackburn,D Inostroza,N.G Gianfreda,L Greiner,R Mora,M.L Jorquera,M.A
author_sort	Menezes-Blackburn,D
title	Phytase-producing Bacillus sp. inoculation increases phosphorus availability in cattle manure
title_short	Phytase-producing Bacillus sp. inoculation increases phosphorus availability in cattle manure
title_full	Phytase-producing Bacillus sp. inoculation increases phosphorus availability in cattle manure
title_fullStr	Phytase-producing Bacillus sp. inoculation increases phosphorus availability in cattle manure
title_full_unstemmed	Phytase-producing Bacillus sp. inoculation increases phosphorus availability in cattle manure
title_sort	phytase-producing bacillus sp. inoculation increases phosphorus availability in cattle manure
publisher	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162016000100016
work_keys_str_mv	AT menezesblackburnd phytaseproducingbacillusspinoculationincreasesphosphorusavailabilityincattlemanure AT inostrozang phytaseproducingbacillusspinoculationincreasesphosphorusavailabilityincattlemanure AT gianfredal phytaseproducingbacillusspinoculationincreasesphosphorusavailabilityincattlemanure AT greinerr phytaseproducingbacillusspinoculationincreasesphosphorusavailabilityincattlemanure AT moraml phytaseproducingbacillusspinoculationincreasesphosphorusavailabilityincattlemanure AT jorquerama phytaseproducingbacillusspinoculationincreasesphosphorusavailabilityincattlemanure
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Phytase-producing Bacillus sp. inoculation increases phosphorus availability in cattle manure

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