Analysis of plant growth promoting potential of endophytes isolated from Echinacea purpurea and Lonicera japonica

Plant endophytes help in maintaining plant health by means of their biofertilizer and biocontrol attributes and, are currently being explored for their ability to produce novel biologically active compounds. Herein, we have isolated beneficial endophytic bacteria from Echinacea purpurea (EF.B3) and...

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Autores principales:	Gupta,H, Saini,R. V, Pagadala,V, Kumar,N, Sharma,D. K, Saini,A. K
Lenguaje:	English
Publicado:	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo 2016
Materias:	antifungal
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162016000300001
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spelling	oai:scielo:S0718-951620160003000012017-02-07Analysis of plant growth promoting potential of endophytes isolated from Echinacea purpurea and Lonicera japonicaGupta,HSaini,R. VPagadala,VKumar,NSharma,D. KSaini,A. K antifungal Plant endophytes help in maintaining plant health by means of their biofertilizer and biocontrol attributes and, are currently being explored for their ability to produce novel biologically active compounds. Herein, we have isolated beneficial endophytic bacteria from Echinacea purpurea (EF.B3) and Lonicera japonica (LS.B11) that showed phosphate solubilization, siderophore, indole acetic acid and hydrogen cyanide production, and fixation of atmospheric nitrogen. Additionally, the endophytes also conferred antifungal activity against Fusarium sp., Rhizoctonia sp., Pythium sp. and Alternaria sp. When tested in plantae, the LS.B11 and EF.B3 strains were able to promote plant growth and control fungal infections in peaseedlings. Both strains were found to be endophytic as tested by RAPD and viability count. Based on 16S rRNA gene sequencing, we show that the LS.B11 and EF.B3 strains are related to Pseudomonas sp. And Burkholderia sp. By using degenerate primers, we identified genes related to polyketide synthases and non-ribosomal peptide synthetases in EF.B3 and LS.B11, respectively that are typically involved in the production of antimicrobial compounds. Therefore, we conclude that both endophytes can be used for increasing agriculture productivity and in the production of antimicrobial compounds for crop improvement.info:eu-repo/semantics/openAccessChilean Society of Soil Science / Sociedad Chilena de la Ciencia del SueloJournal of soil science and plant nutrition v.16 n.3 20162016-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162016000300001en10.4067/S0718-95162016005000025
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	antifungal
spellingShingle	antifungal Gupta,H Saini,R. V Pagadala,V Kumar,N Sharma,D. K Saini,A. K Analysis of plant growth promoting potential of endophytes isolated from Echinacea purpurea and Lonicera japonica
description	Plant endophytes help in maintaining plant health by means of their biofertilizer and biocontrol attributes and, are currently being explored for their ability to produce novel biologically active compounds. Herein, we have isolated beneficial endophytic bacteria from Echinacea purpurea (EF.B3) and Lonicera japonica (LS.B11) that showed phosphate solubilization, siderophore, indole acetic acid and hydrogen cyanide production, and fixation of atmospheric nitrogen. Additionally, the endophytes also conferred antifungal activity against Fusarium sp., Rhizoctonia sp., Pythium sp. and Alternaria sp. When tested in plantae, the LS.B11 and EF.B3 strains were able to promote plant growth and control fungal infections in peaseedlings. Both strains were found to be endophytic as tested by RAPD and viability count. Based on 16S rRNA gene sequencing, we show that the LS.B11 and EF.B3 strains are related to Pseudomonas sp. And Burkholderia sp. By using degenerate primers, we identified genes related to polyketide synthases and non-ribosomal peptide synthetases in EF.B3 and LS.B11, respectively that are typically involved in the production of antimicrobial compounds. Therefore, we conclude that both endophytes can be used for increasing agriculture productivity and in the production of antimicrobial compounds for crop improvement.
author	Gupta,H Saini,R. V Pagadala,V Kumar,N Sharma,D. K Saini,A. K
author_facet	Gupta,H Saini,R. V Pagadala,V Kumar,N Sharma,D. K Saini,A. K
author_sort	Gupta,H
title	Analysis of plant growth promoting potential of endophytes isolated from Echinacea purpurea and Lonicera japonica
title_short	Analysis of plant growth promoting potential of endophytes isolated from Echinacea purpurea and Lonicera japonica
title_full	Analysis of plant growth promoting potential of endophytes isolated from Echinacea purpurea and Lonicera japonica
title_fullStr	Analysis of plant growth promoting potential of endophytes isolated from Echinacea purpurea and Lonicera japonica
title_full_unstemmed	Analysis of plant growth promoting potential of endophytes isolated from Echinacea purpurea and Lonicera japonica
title_sort	analysis of plant growth promoting potential of endophytes isolated from echinacea purpurea and lonicera japonica
publisher	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162016000300001
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Analysis of plant growth promoting potential of endophytes isolated from Echinacea purpurea and Lonicera japonica

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