Modulation of antioxidant enzymes by salicylic acid in arsenic exposed Glycine max L.

To investigate the physiological and metabolic attributes of arsenic (As) stress tolerance conferred by exogenous salicylic acid (SA), Glycine max L. (variety JS 335) seeds were aseptically germinated over filter paper moistened with SA (500 µM) and/or10 and 100 µM As (Sodium arsenite was used). On...

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Autores principales:	Chandrakar,V, Dubey,A, Keshavkant,S
Lenguaje:	English
Publicado:	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo 2016
Materias:	Antioxidant enzymes arsenic toxicity oxidative stress reactive oxygen species salicylic acid
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162016000300008
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spelling	oai:scielo:S0718-951620160003000082017-02-07Modulation of antioxidant enzymes by salicylic acid in arsenic exposed Glycine max L.Chandrakar,VDubey,AKeshavkant,S Antioxidant enzymes arsenic toxicity oxidative stress reactive oxygen species salicylic acid To investigate the physiological and metabolic attributes of arsenic (As) stress tolerance conferred by exogenous salicylic acid (SA), Glycine max L. (variety JS 335) seeds were aseptically germinated over filter paper moistened with SA (500 µM) and/or10 and 100 µM As (Sodium arsenite was used). On 2nd and 5th days of germination, the growing radicles were harvested, and analyzed for growth and different metabolic attributes. Findings exemplified that As significantly decreased germination percentage, radicle length, dry mass and activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX), while stimulated the contents of As, reactive oxygen species (ROS), lipoxygenase (LOX), guaiacol peroxidase (POD) and proline. Additionally, isozymes of antioxidants were also scrutinized over Native-PAGE gels and were found to be altered considerably under As-stress. However, exogenous SA remarkably enhanced germination percentage, growth indices, activities of SOD, CAT and APX, and proline accumulation along with reduced As, ROS and LOX, and restoring POD in As-stressed seedlings. In conclusion, SA confers As-stress tolerance to Glycine max L. by regulating the antioxidant enzymes and proline accumulation thereby reduced As content and ROS production. Further study is intended, particularly at gene level, to understand precise mechanism(s) involved in SA-mediated As-stress tolerance.info:eu-repo/semantics/openAccessChilean Society of Soil Science / Sociedad Chilena de la Ciencia del SueloJournal of soil science and plant nutrition v.16 n.3 20162016-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162016000300008en10.4067/S0718-95162016005000048
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Antioxidant enzymes arsenic toxicity oxidative stress reactive oxygen species salicylic acid
spellingShingle	Antioxidant enzymes arsenic toxicity oxidative stress reactive oxygen species salicylic acid Chandrakar,V Dubey,A Keshavkant,S Modulation of antioxidant enzymes by salicylic acid in arsenic exposed Glycine max L.
description	To investigate the physiological and metabolic attributes of arsenic (As) stress tolerance conferred by exogenous salicylic acid (SA), Glycine max L. (variety JS 335) seeds were aseptically germinated over filter paper moistened with SA (500 µM) and/or10 and 100 µM As (Sodium arsenite was used). On 2nd and 5th days of germination, the growing radicles were harvested, and analyzed for growth and different metabolic attributes. Findings exemplified that As significantly decreased germination percentage, radicle length, dry mass and activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX), while stimulated the contents of As, reactive oxygen species (ROS), lipoxygenase (LOX), guaiacol peroxidase (POD) and proline. Additionally, isozymes of antioxidants were also scrutinized over Native-PAGE gels and were found to be altered considerably under As-stress. However, exogenous SA remarkably enhanced germination percentage, growth indices, activities of SOD, CAT and APX, and proline accumulation along with reduced As, ROS and LOX, and restoring POD in As-stressed seedlings. In conclusion, SA confers As-stress tolerance to Glycine max L. by regulating the antioxidant enzymes and proline accumulation thereby reduced As content and ROS production. Further study is intended, particularly at gene level, to understand precise mechanism(s) involved in SA-mediated As-stress tolerance.
author	Chandrakar,V Dubey,A Keshavkant,S
author_facet	Chandrakar,V Dubey,A Keshavkant,S
author_sort	Chandrakar,V
title	Modulation of antioxidant enzymes by salicylic acid in arsenic exposed Glycine max L.
title_short	Modulation of antioxidant enzymes by salicylic acid in arsenic exposed Glycine max L.
title_full	Modulation of antioxidant enzymes by salicylic acid in arsenic exposed Glycine max L.
title_fullStr	Modulation of antioxidant enzymes by salicylic acid in arsenic exposed Glycine max L.
title_full_unstemmed	Modulation of antioxidant enzymes by salicylic acid in arsenic exposed Glycine max L.
title_sort	modulation of antioxidant enzymes by salicylic acid in arsenic exposed glycine max l.
publisher	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162016000300008
work_keys_str_mv	AT chandrakarv modulationofantioxidantenzymesbysalicylicacidinarsenicexposedglycinemaxl AT dubeya modulationofantioxidantenzymesbysalicylicacidinarsenicexposedglycinemaxl AT keshavkants modulationofantioxidantenzymesbysalicylicacidinarsenicexposedglycinemaxl
_version_	1714206538997956608

Modulation of antioxidant enzymes by salicylic acid in arsenic exposed Glycine max L.

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