Abundance of denitrifying genes and microbial community structure in volcanic soils

Nitrous oxide (N2O) is a potent greenhouse gas produced during denitrification, a process that includes several genes coding enzymes responsible for nitrogen (N) forms transformations. In volcanic ash soils of southern Chile, fertilization had increased over the last 10 years with implications for N...

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Autores principales:	Carvajal,A.M, Vargas,R.A, Alfaro,M
Lenguaje:	English
Publicado:	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo 2016
Materias:	Volcanic soils denitrification nitrous oxide denitrifying genes microbial diversity
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162016000300009
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spelling	oai:scielo:S0718-951620160003000092017-02-07Abundance of denitrifying genes and microbial community structure in volcanic soilsCarvajal,A.MVargas,R.AAlfaro,M Volcanic soils denitrification nitrous oxide denitrifying genes microbial diversity Nitrous oxide (N2O) is a potent greenhouse gas produced during denitrification, a process that includes several genes coding enzymes responsible for nitrogen (N) forms transformations. In volcanic ash soils of southern Chile, fertilization had increased over the last 10 years with implications for N2O emissions. Despite this, little is still known about potential denitrification and the presence of denitrifying genes. In this study we report the abundance of denitrifying genes and a basal characterization of microbial communities in five volcanic ash soils with different levels of organic matter. The denitrifying genes determination showed that nosZI predominated over nirK and nirS in all soils suggesting a complete denitrification pathway, which would explain low N2O losses in such soils. Estimation of total microorganisms studies using 16S and 18S rRNA showed that in these soils bacteria (10(9)) was most abundant over archaea and fungi (10(7); p<0.05). Sequences of DNA obtained by denaturant gradient gel electrophoresis (DGGE) indicated that Firmicutes, Acidobacteria and Proteobacteria are the main bacterial classes represented in Andisoils (33, 28 and 23%, respectively) but with a lower diversity. More detailed studies about denitrifiers, gene-activity relationship and N2O emissions are required.info:eu-repo/semantics/openAccessChilean Society of Soil Science / Sociedad Chilena de la Ciencia del SueloJournal of soil science and plant nutrition v.16 n.3 20162016-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162016000300009en10.4067/S0718-95162016005000049
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Volcanic soils denitrification nitrous oxide denitrifying genes microbial diversity
spellingShingle	Volcanic soils denitrification nitrous oxide denitrifying genes microbial diversity Carvajal,A.M Vargas,R.A Alfaro,M Abundance of denitrifying genes and microbial community structure in volcanic soils
description	Nitrous oxide (N2O) is a potent greenhouse gas produced during denitrification, a process that includes several genes coding enzymes responsible for nitrogen (N) forms transformations. In volcanic ash soils of southern Chile, fertilization had increased over the last 10 years with implications for N2O emissions. Despite this, little is still known about potential denitrification and the presence of denitrifying genes. In this study we report the abundance of denitrifying genes and a basal characterization of microbial communities in five volcanic ash soils with different levels of organic matter. The denitrifying genes determination showed that nosZI predominated over nirK and nirS in all soils suggesting a complete denitrification pathway, which would explain low N2O losses in such soils. Estimation of total microorganisms studies using 16S and 18S rRNA showed that in these soils bacteria (10(9)) was most abundant over archaea and fungi (10(7); p<0.05). Sequences of DNA obtained by denaturant gradient gel electrophoresis (DGGE) indicated that Firmicutes, Acidobacteria and Proteobacteria are the main bacterial classes represented in Andisoils (33, 28 and 23%, respectively) but with a lower diversity. More detailed studies about denitrifiers, gene-activity relationship and N2O emissions are required.
author	Carvajal,A.M Vargas,R.A Alfaro,M
author_facet	Carvajal,A.M Vargas,R.A Alfaro,M
author_sort	Carvajal,A.M
title	Abundance of denitrifying genes and microbial community structure in volcanic soils
title_short	Abundance of denitrifying genes and microbial community structure in volcanic soils
title_full	Abundance of denitrifying genes and microbial community structure in volcanic soils
title_fullStr	Abundance of denitrifying genes and microbial community structure in volcanic soils
title_full_unstemmed	Abundance of denitrifying genes and microbial community structure in volcanic soils
title_sort	abundance of denitrifying genes and microbial community structure in volcanic soils
publisher	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162016000300009
work_keys_str_mv	AT carvajalam abundanceofdenitrifyinggenesandmicrobialcommunitystructureinvolcanicsoils AT vargasra abundanceofdenitrifyinggenesandmicrobialcommunitystructureinvolcanicsoils AT alfarom abundanceofdenitrifyinggenesandmicrobialcommunitystructureinvolcanicsoils
_version_	1714206539161534464

Abundance of denitrifying genes and microbial community structure in volcanic soils

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