Characterization of yeast in hapludands soil with biotechnological potential

Yeasts play an important role in nature, and soil is one of the largest reservoirs of yeasts. Seventy-seven yeast strains were isolated from southern Chilean volcanic ash soil. These 77 strains were composed of 30 strains derived from soils used as permanent grassland, 30 strains from rotating grass...

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Autores principales:	Díaz,Paola E, Aranda,Camila, Martínez,Oscar, Godoy,Roberto, Gonzales,Aldo, Valenzuela,Eduardo
Lenguaje:	English
Publicado:	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo 2017
Materias:	Yeast PCR-RFLP ITS-5.8S lipids biofertilizers
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162017000400009
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spelling	oai:scielo:S0718-951620170004000092017-12-20Characterization of yeast in hapludands soil with biotechnological potentialDíaz,Paola EAranda,CamilaMartínez,OscarGodoy,RobertoGonzales,AldoValenzuela,Eduardo Yeast PCR-RFLP ITS-5.8S lipids biofertilizers Yeasts play an important role in nature, and soil is one of the largest reservoirs of yeasts. Seventy-seven yeast strains were isolated from southern Chilean volcanic ash soil. These 77 strains were composed of 30 strains derived from soils used as permanent grassland, 30 strains from rotating grassland, and 17 strains from native forest (considered as the control). These strains were identified using restriction fragment length polymorphisms (PCR-RFLP) in conjunction with ITS-5.8S rDNA sequencing. Additionally, a physiological characterization of the assimilation of carbon and nitrogen and the fermentation of sugars was performed. Various features of the strains were evaluated including their growth in vinasse medium, nitrogen and phosphorus content, and lipid production. Lastly, the use of strains as biofertilizers was analyzed via the cultivation of bell pepper seedlings in soil. From the RFLP profiles, the 77 strains clustered into 10 groups. Of these, only three groups could be identified to the species level, and another was classified to the level of genus (Devaryomyces hansenii, Pichia fermentan, Kazachstania exigua, Candida sp.). All strains grew in vinasse medium. Six strains (PP1, PP4, PR4, PR10, PR27 and PR29) showed a high capacity to accumulate lipids, and three strains (PP1, PP21 and PR28) have the potential to be used as biofertilizers.info:eu-repo/semantics/openAccessChilean Society of Soil Science / Sociedad Chilena de la Ciencia del SueloJournal of soil science and plant nutrition v.17 n.4 20172017-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162017000400009en10.4067/S0718-95162017000400009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Yeast PCR-RFLP ITS-5.8S lipids biofertilizers
spellingShingle	Yeast PCR-RFLP ITS-5.8S lipids biofertilizers Díaz,Paola E Aranda,Camila Martínez,Oscar Godoy,Roberto Gonzales,Aldo Valenzuela,Eduardo Characterization of yeast in hapludands soil with biotechnological potential
description	Yeasts play an important role in nature, and soil is one of the largest reservoirs of yeasts. Seventy-seven yeast strains were isolated from southern Chilean volcanic ash soil. These 77 strains were composed of 30 strains derived from soils used as permanent grassland, 30 strains from rotating grassland, and 17 strains from native forest (considered as the control). These strains were identified using restriction fragment length polymorphisms (PCR-RFLP) in conjunction with ITS-5.8S rDNA sequencing. Additionally, a physiological characterization of the assimilation of carbon and nitrogen and the fermentation of sugars was performed. Various features of the strains were evaluated including their growth in vinasse medium, nitrogen and phosphorus content, and lipid production. Lastly, the use of strains as biofertilizers was analyzed via the cultivation of bell pepper seedlings in soil. From the RFLP profiles, the 77 strains clustered into 10 groups. Of these, only three groups could be identified to the species level, and another was classified to the level of genus (Devaryomyces hansenii, Pichia fermentan, Kazachstania exigua, Candida sp.). All strains grew in vinasse medium. Six strains (PP1, PP4, PR4, PR10, PR27 and PR29) showed a high capacity to accumulate lipids, and three strains (PP1, PP21 and PR28) have the potential to be used as biofertilizers.
author	Díaz,Paola E Aranda,Camila Martínez,Oscar Godoy,Roberto Gonzales,Aldo Valenzuela,Eduardo
author_facet	Díaz,Paola E Aranda,Camila Martínez,Oscar Godoy,Roberto Gonzales,Aldo Valenzuela,Eduardo
author_sort	Díaz,Paola E
title	Characterization of yeast in hapludands soil with biotechnological potential
title_short	Characterization of yeast in hapludands soil with biotechnological potential
title_full	Characterization of yeast in hapludands soil with biotechnological potential
title_fullStr	Characterization of yeast in hapludands soil with biotechnological potential
title_full_unstemmed	Characterization of yeast in hapludands soil with biotechnological potential
title_sort	characterization of yeast in hapludands soil with biotechnological potential
publisher	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162017000400009
work_keys_str_mv	AT diazpaolae characterizationofyeastinhapludandssoilwithbiotechnologicalpotential AT arandacamila characterizationofyeastinhapludandssoilwithbiotechnologicalpotential AT martinezoscar characterizationofyeastinhapludandssoilwithbiotechnologicalpotential AT godoyroberto characterizationofyeastinhapludandssoilwithbiotechnologicalpotential AT gonzalesaldo characterizationofyeastinhapludandssoilwithbiotechnologicalpotential AT valenzuelaeduardo characterizationofyeastinhapludandssoilwithbiotechnologicalpotential
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Characterization of yeast in hapludands soil with biotechnological potential

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