Novel hydrocarbonoclastic metal-tolerant Acinetobacter and Pseudomonas strains from Aconcagua river oil-polluted soil

Bioremediation of sites polluted with petroleum hydrocarbons and heavy metals is a major challenge. The aim of this study was the isolation and characterization of hydrocarbon-degrading and heavy metal-tolerant bacteria. Sixteen hydrocarbonoclastic bacteria were isolated by enrichment from a crude o...

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Autores principales:	Méndez,Valentina, Fuentes,Sebastián, Morgante,Verónica, Hernández,Marcela, González,Myriam, Moore,Edward, Seeger,Michael
Lenguaje:	English
Publicado:	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo 2017
Materias:	Biodegradation hydrocarbon heavy metal PAH
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162017000400017
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spelling	oai:scielo:S0718-951620170004000172017-12-20Novel hydrocarbonoclastic metal-tolerant Acinetobacter and Pseudomonas strains from Aconcagua river oil-polluted soilMéndez,ValentinaFuentes,SebastiánMorgante,VerónicaHernández,MarcelaGonzález,MyriamMoore,EdwardSeeger,Michael Biodegradation hydrocarbon heavy metal PAH Bioremediation of sites polluted with petroleum hydrocarbons and heavy metals is a major challenge. The aim of this study was the isolation and characterization of hydrocarbon-degrading and heavy metal-tolerant bacteria. Sixteen hydrocarbonoclastic bacteria were isolated by enrichment from a crude oil-contaminated soil at Aconcagua river mouth, central Chile. Most strains were cocci-shaped and exhibited circular cream-colored colonies with smooth texture. Isolates were resistant to bacitracin and penicillin, and two isolates were motile. Isolates were identified by 16S rRNA and rpoD and rpoB genes sequence analyses. Most isolates belonged to Gammaproteobacteria including Acinetobacter radioresistens (4 isolates), Acinetobacter calcoaceticus (1), Pseudomonas stutzeri (2)and Pseudomonas chloritidismutans (1). Seven isolates possessed 97% 16S rRNA gene similarity with A. calcoaceticus ATCC 23055T, suggesting that these probably represent a new Acinetobacter species. One isolate is an Actinobacteria of the Kocuria genus. All isolates were able to grow on crude oil, whereas eleven Acinetobacter and Pseudomonas strains grew on n-hexadecane. Pseudomonas isolates grew on fluorene (DM88 and DM95) and naphthalene (DD74). Acinetobacter isolates grew on fluorene (DD75, DD79 and DM81) and phenanthrene (DM82). Remarkably, most isolates (except DD79) exhibited copper or cadmium tolerance. These novel hydrocarbonoclastic and heavy metal-tolerant Pseudomonas and Acinetobacter strains are potential biocatalysts for bioremediation.info:eu-repo/semantics/openAccessChilean Society of Soil Science / Sociedad Chilena de la Ciencia del SueloJournal of soil science and plant nutrition v.17 n.4 20172017-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162017000400017en10.4067/S0718-95162017000400017
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Biodegradation hydrocarbon heavy metal PAH
spellingShingle	Biodegradation hydrocarbon heavy metal PAH Méndez,Valentina Fuentes,Sebastián Morgante,Verónica Hernández,Marcela González,Myriam Moore,Edward Seeger,Michael Novel hydrocarbonoclastic metal-tolerant Acinetobacter and Pseudomonas strains from Aconcagua river oil-polluted soil
description	Bioremediation of sites polluted with petroleum hydrocarbons and heavy metals is a major challenge. The aim of this study was the isolation and characterization of hydrocarbon-degrading and heavy metal-tolerant bacteria. Sixteen hydrocarbonoclastic bacteria were isolated by enrichment from a crude oil-contaminated soil at Aconcagua river mouth, central Chile. Most strains were cocci-shaped and exhibited circular cream-colored colonies with smooth texture. Isolates were resistant to bacitracin and penicillin, and two isolates were motile. Isolates were identified by 16S rRNA and rpoD and rpoB genes sequence analyses. Most isolates belonged to Gammaproteobacteria including Acinetobacter radioresistens (4 isolates), Acinetobacter calcoaceticus (1), Pseudomonas stutzeri (2)and Pseudomonas chloritidismutans (1). Seven isolates possessed 97% 16S rRNA gene similarity with A. calcoaceticus ATCC 23055T, suggesting that these probably represent a new Acinetobacter species. One isolate is an Actinobacteria of the Kocuria genus. All isolates were able to grow on crude oil, whereas eleven Acinetobacter and Pseudomonas strains grew on n-hexadecane. Pseudomonas isolates grew on fluorene (DM88 and DM95) and naphthalene (DD74). Acinetobacter isolates grew on fluorene (DD75, DD79 and DM81) and phenanthrene (DM82). Remarkably, most isolates (except DD79) exhibited copper or cadmium tolerance. These novel hydrocarbonoclastic and heavy metal-tolerant Pseudomonas and Acinetobacter strains are potential biocatalysts for bioremediation.
author	Méndez,Valentina Fuentes,Sebastián Morgante,Verónica Hernández,Marcela González,Myriam Moore,Edward Seeger,Michael
author_facet	Méndez,Valentina Fuentes,Sebastián Morgante,Verónica Hernández,Marcela González,Myriam Moore,Edward Seeger,Michael
author_sort	Méndez,Valentina
title	Novel hydrocarbonoclastic metal-tolerant Acinetobacter and Pseudomonas strains from Aconcagua river oil-polluted soil
title_short	Novel hydrocarbonoclastic metal-tolerant Acinetobacter and Pseudomonas strains from Aconcagua river oil-polluted soil
title_full	Novel hydrocarbonoclastic metal-tolerant Acinetobacter and Pseudomonas strains from Aconcagua river oil-polluted soil
title_fullStr	Novel hydrocarbonoclastic metal-tolerant Acinetobacter and Pseudomonas strains from Aconcagua river oil-polluted soil
title_full_unstemmed	Novel hydrocarbonoclastic metal-tolerant Acinetobacter and Pseudomonas strains from Aconcagua river oil-polluted soil
title_sort	novel hydrocarbonoclastic metal-tolerant acinetobacter and pseudomonas strains from aconcagua river oil-polluted soil
publisher	Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162017000400017
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Novel hydrocarbonoclastic metal-tolerant Acinetobacter and Pseudomonas strains from Aconcagua river oil-polluted soil

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