Single Exposure of Human Oral Mucosa Fibroblasts to Ultraviolet B Radiation Reduces Proliferation and Induces COX-2 Expression and Activation

The lip vermillion constitutes a transition tissue, between oral mucosa and skin, where oral mucosal cells from epithelial and connective tissue compartments are exposed to ultraviolet (UV) sunlight. Fibroblasts are abundant resident cells of the connective tissue which are key regulators of extrace...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Boza,Y, Yefi,R, Rudolph,Ml, Smith,PC, Oberyszyn,TM, Tober,KL, Rojas,IG
Lenguaje:English
Publicado: Sociedad de Periodoncia de Chile. Sociedad de Implantología Oral de Chile. Sociedad de Prótesis y Rehabilitación Oral de Chile. 2010
Materias:
UVB
PGE
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-01072010000300004
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:scielo:S0719-01072010000300004
record_format dspace
spelling oai:scielo:S0719-010720100003000042012-02-03Single Exposure of Human Oral Mucosa Fibroblasts to Ultraviolet B Radiation Reduces Proliferation and Induces COX-2 Expression and ActivationBoza,YYefi,RRudolph,MlSmith,PCOberyszyn,TMTober,KLRojas,IG Oral fibroblasts UVB COX-2 proliferation PGE The lip vermillion constitutes a transition tissue, between oral mucosa and skin, where oral mucosal cells from epithelial and connective tissue compartments are exposed to ultraviolet (UV) sunlight. Fibroblasts are abundant resident cells of the connective tissue which are key regulators of extracellular matrix composition, as well as, epithelial and endothelial cell function. UVB light, an inherent component of sunlight, causes several alterations in skin fibroblasts, including premature senescence and increased cyclooxygenase (COX)-2 expression. To assess if UVB irradiation had similar effects on fibroblasts derived from human oral mucosa (HOM), primary cultures of HOM fibroblasts were irradiated with a single dose of 30 or 60 mJ/cm²of UVB light or sham-irradiated. Fibroblast proliferation was assessed from 3 to 48 hrs after UVB-irradiation utilizing [³H]-thymidine incorporation and MTT assays. In addition, COX-2 mRNA expression was detected by RT-PCR, and PGE2 production was assessed using enzyme immunoassay from 0.5 to 24 hrs after UVB-irradiation. The results showed a significant decrease in proliferation of UVB-irradiated HOM fibroblasts as compared to controls as measured by both [³H]-thymidine incorporation and MTT assays (p<0.001). HOM fibroblasts had increased COX-2 mRNA expression at 0.5 and 12 hrs after irradiation, and PGE2 production was elevated at 12 and 24 hrs post-irradiation as compared to controls (p<0.05). The results showed an inhibitory effect of a single dose of UVB irradiation on HOM fibroblast proliferation with an increase in COX-2 expression and activation. Therefore, photodamaged fibroblasts may play and important role in the pathogenesis of UV-induced lesions of the lip.info:eu-repo/semantics/openAccessSociedad de Periodoncia de Chile. Sociedad de Implantología Oral de Chile. Sociedad de Prótesis y Rehabilitación Oral de Chile.Revista clínica de periodoncia, implantología y rehabilitación oral v.3 n.3 20102010-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-01072010000300004en
institution Scielo Chile
collection Scielo Chile
language English
topic Oral fibroblasts
UVB
COX-2
proliferation
PGE
spellingShingle Oral fibroblasts
UVB
COX-2
proliferation
PGE
Boza,Y
Yefi,R
Rudolph,Ml
Smith,PC
Oberyszyn,TM
Tober,KL
Rojas,IG
Single Exposure of Human Oral Mucosa Fibroblasts to Ultraviolet B Radiation Reduces Proliferation and Induces COX-2 Expression and Activation
description The lip vermillion constitutes a transition tissue, between oral mucosa and skin, where oral mucosal cells from epithelial and connective tissue compartments are exposed to ultraviolet (UV) sunlight. Fibroblasts are abundant resident cells of the connective tissue which are key regulators of extracellular matrix composition, as well as, epithelial and endothelial cell function. UVB light, an inherent component of sunlight, causes several alterations in skin fibroblasts, including premature senescence and increased cyclooxygenase (COX)-2 expression. To assess if UVB irradiation had similar effects on fibroblasts derived from human oral mucosa (HOM), primary cultures of HOM fibroblasts were irradiated with a single dose of 30 or 60 mJ/cm²of UVB light or sham-irradiated. Fibroblast proliferation was assessed from 3 to 48 hrs after UVB-irradiation utilizing [³H]-thymidine incorporation and MTT assays. In addition, COX-2 mRNA expression was detected by RT-PCR, and PGE2 production was assessed using enzyme immunoassay from 0.5 to 24 hrs after UVB-irradiation. The results showed a significant decrease in proliferation of UVB-irradiated HOM fibroblasts as compared to controls as measured by both [³H]-thymidine incorporation and MTT assays (p<0.001). HOM fibroblasts had increased COX-2 mRNA expression at 0.5 and 12 hrs after irradiation, and PGE2 production was elevated at 12 and 24 hrs post-irradiation as compared to controls (p<0.05). The results showed an inhibitory effect of a single dose of UVB irradiation on HOM fibroblast proliferation with an increase in COX-2 expression and activation. Therefore, photodamaged fibroblasts may play and important role in the pathogenesis of UV-induced lesions of the lip.
author Boza,Y
Yefi,R
Rudolph,Ml
Smith,PC
Oberyszyn,TM
Tober,KL
Rojas,IG
author_facet Boza,Y
Yefi,R
Rudolph,Ml
Smith,PC
Oberyszyn,TM
Tober,KL
Rojas,IG
author_sort Boza,Y
title Single Exposure of Human Oral Mucosa Fibroblasts to Ultraviolet B Radiation Reduces Proliferation and Induces COX-2 Expression and Activation
title_short Single Exposure of Human Oral Mucosa Fibroblasts to Ultraviolet B Radiation Reduces Proliferation and Induces COX-2 Expression and Activation
title_full Single Exposure of Human Oral Mucosa Fibroblasts to Ultraviolet B Radiation Reduces Proliferation and Induces COX-2 Expression and Activation
title_fullStr Single Exposure of Human Oral Mucosa Fibroblasts to Ultraviolet B Radiation Reduces Proliferation and Induces COX-2 Expression and Activation
title_full_unstemmed Single Exposure of Human Oral Mucosa Fibroblasts to Ultraviolet B Radiation Reduces Proliferation and Induces COX-2 Expression and Activation
title_sort single exposure of human oral mucosa fibroblasts to ultraviolet b radiation reduces proliferation and induces cox-2 expression and activation
publisher Sociedad de Periodoncia de Chile. Sociedad de Implantología Oral de Chile. Sociedad de Prótesis y Rehabilitación Oral de Chile.
publishDate 2010
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-01072010000300004
work_keys_str_mv AT bozay singleexposureofhumanoralmucosafibroblaststoultravioletbradiationreducesproliferationandinducescox2expressionandactivation
AT yefir singleexposureofhumanoralmucosafibroblaststoultravioletbradiationreducesproliferationandinducescox2expressionandactivation
AT rudolphml singleexposureofhumanoralmucosafibroblaststoultravioletbradiationreducesproliferationandinducescox2expressionandactivation
AT smithpc singleexposureofhumanoralmucosafibroblaststoultravioletbradiationreducesproliferationandinducescox2expressionandactivation
AT oberyszyntm singleexposureofhumanoralmucosafibroblaststoultravioletbradiationreducesproliferationandinducescox2expressionandactivation
AT toberkl singleexposureofhumanoralmucosafibroblaststoultravioletbradiationreducesproliferationandinducescox2expressionandactivation
AT rojasig singleexposureofhumanoralmucosafibroblaststoultravioletbradiationreducesproliferationandinducescox2expressionandactivation
_version_ 1714206665585197056