AN EFFICIENT PROTOCOL TO PERFORM GENETIC TRACEABILITY OF TISSUE AND FOODS FROM Geoffroea decorticans

ABSTRACT The quality of a DNA isolation method depends, among others, on the target tissue and the metabolites therein. Geoffroea decorticans Burkart (chañar) is a species that has nutritional and pharmacological potential. However, an effective method of DNA extraction capable of facilitating popul...

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Autores principales:	Contreras,Roberto, Porcile,Vincenzo, Guggiana-Nilo,Drago, Aguayo,Fernanda
Lenguaje:	English
Publicado:	Universidad de Concepción. Facultad de Agronomía, Facultad de Ingeniería Agricola y Facultad de Ciencias Veterinarias 2019
Materias:	DNA isolation chañar Geoffroea decorticans quality control ISSR RAPD SSR
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-38902019000300224
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spelling	oai:scielo:S0719-389020190003002242020-01-29AN EFFICIENT PROTOCOL TO PERFORM GENETIC TRACEABILITY OF TISSUE AND FOODS FROM Geoffroea decorticansContreras,RobertoPorcile,VincenzoGuggiana-Nilo,DragoAguayo,Fernanda DNA isolation chañar Geoffroea decorticans quality control ISSR RAPD SSR ABSTRACT The quality of a DNA isolation method depends, among others, on the target tissue and the metabolites therein. Geoffroea decorticans Burkart (chañar) is a species that has nutritional and pharmacological potential. However, an effective method of DNA extraction capable of facilitating population studies and food genetic traceability has not been studied yet. The objective of the present work was to evaluate four methods of DNA extraction from leaves and chañar-based foods. The methods were evaluated based on yield, DNA purity, and molecular markers. The CCI-p (CTAB/ Chloroform-Isoamylalcohol/pellet) method showed the highest yield of DNA obtained from leaves. However, the CPCI-SC (CTAB/phenol-Chloroform-Isoamylalcohol/silica-column) method was the only one that resulted in acceptable DNA quality with both parameters (A260/A280 and A260/A230). The leaf DNA obtained with this method showed a greater amount of fragments with RAPD, and an acceptable amount of fragments with ISSR. On the other hand, the CCI-p method showed a higher yield of DNA from arrope de chañar (syrup). However, the CPCI-SC method was the only one that had relatively better DNA quality, which allowed the amplification of molecular markers. Regarding chañar flour, the CPCI-SC method showed the highest yield, DNA quality and good amplification with molecular markers. Therefore, the CPCI-SC extraction method is efficient for obtaining DNA from different matrices, and can support studies for a possible designation of origin of chañar-based foods.info:eu-repo/semantics/openAccessUniversidad de Concepción. Facultad de Agronomía, Facultad de Ingeniería Agricola y Facultad de Ciencias VeterinariasChilean journal of agricultural & animal sciences v.35 n.3 20192019-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-38902019000300224en10.4067/S0719-38902019005000402
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	DNA isolation chañar Geoffroea decorticans quality control ISSR RAPD SSR
spellingShingle	DNA isolation chañar Geoffroea decorticans quality control ISSR RAPD SSR Contreras,Roberto Porcile,Vincenzo Guggiana-Nilo,Drago Aguayo,Fernanda AN EFFICIENT PROTOCOL TO PERFORM GENETIC TRACEABILITY OF TISSUE AND FOODS FROM Geoffroea decorticans
description	ABSTRACT The quality of a DNA isolation method depends, among others, on the target tissue and the metabolites therein. Geoffroea decorticans Burkart (chañar) is a species that has nutritional and pharmacological potential. However, an effective method of DNA extraction capable of facilitating population studies and food genetic traceability has not been studied yet. The objective of the present work was to evaluate four methods of DNA extraction from leaves and chañar-based foods. The methods were evaluated based on yield, DNA purity, and molecular markers. The CCI-p (CTAB/ Chloroform-Isoamylalcohol/pellet) method showed the highest yield of DNA obtained from leaves. However, the CPCI-SC (CTAB/phenol-Chloroform-Isoamylalcohol/silica-column) method was the only one that resulted in acceptable DNA quality with both parameters (A260/A280 and A260/A230). The leaf DNA obtained with this method showed a greater amount of fragments with RAPD, and an acceptable amount of fragments with ISSR. On the other hand, the CCI-p method showed a higher yield of DNA from arrope de chañar (syrup). However, the CPCI-SC method was the only one that had relatively better DNA quality, which allowed the amplification of molecular markers. Regarding chañar flour, the CPCI-SC method showed the highest yield, DNA quality and good amplification with molecular markers. Therefore, the CPCI-SC extraction method is efficient for obtaining DNA from different matrices, and can support studies for a possible designation of origin of chañar-based foods.
author	Contreras,Roberto Porcile,Vincenzo Guggiana-Nilo,Drago Aguayo,Fernanda
author_facet	Contreras,Roberto Porcile,Vincenzo Guggiana-Nilo,Drago Aguayo,Fernanda
author_sort	Contreras,Roberto
title	AN EFFICIENT PROTOCOL TO PERFORM GENETIC TRACEABILITY OF TISSUE AND FOODS FROM Geoffroea decorticans
title_short	AN EFFICIENT PROTOCOL TO PERFORM GENETIC TRACEABILITY OF TISSUE AND FOODS FROM Geoffroea decorticans
title_full	AN EFFICIENT PROTOCOL TO PERFORM GENETIC TRACEABILITY OF TISSUE AND FOODS FROM Geoffroea decorticans
title_fullStr	AN EFFICIENT PROTOCOL TO PERFORM GENETIC TRACEABILITY OF TISSUE AND FOODS FROM Geoffroea decorticans
title_full_unstemmed	AN EFFICIENT PROTOCOL TO PERFORM GENETIC TRACEABILITY OF TISSUE AND FOODS FROM Geoffroea decorticans
title_sort	efficient protocol to perform genetic traceability of tissue and foods from geoffroea decorticans
publisher	Universidad de Concepción. Facultad de Agronomía, Facultad de Ingeniería Agricola y Facultad de Ciencias Veterinarias
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-38902019000300224
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AN EFFICIENT PROTOCOL TO PERFORM GENETIC TRACEABILITY OF TISSUE AND FOODS FROM Geoffroea decorticans

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