Effects of two platelet-rich gel supernatants at two concentrations on healthy cartilage explants from horses

ABSTRACT Platelet-rich plasma is used as a treatment of arthropathies in horses. However, there is lack of in vitro information about the mechanism of action of this substance and its effects on healthy cartilage of these animals. The aims were: (1) to evaluate the effects at 48 and 96 h of two conc...

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Autores principales:	Carmona,Jorge U., Ríos,Diana L., López,Catalina
Lenguaje:	English
Publicado:	Universidad Austral de Chile. Facultad de Ciencias Veterinarias 2017
Materias:	cartilage cytokines growth factors horse platelet-rich plasma.
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322017000100104
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spelling	oai:scielo:S0719-813220170001001042019-11-28Effects of two platelet-rich gel supernatants at two concentrations on healthy cartilage explants from horsesCarmona,Jorge U.Ríos,Diana L.López,Catalina cartilage cytokines growth factors horse platelet-rich plasma. ABSTRACT Platelet-rich plasma is used as a treatment of arthropathies in horses. However, there is lack of in vitro information about the mechanism of action of this substance and its effects on healthy cartilage of these animals. The aims were: (1) to evaluate the effects at 48 and 96 h of two concentrations (25 and 50%) of leukocyte- and platelet-rich gel (L-PRG) and pure platelet rich gel (P-PRG) supernatants on the production/degradation in normal cartilage explants (CEs) of platelet-derived growth factor isoform BB, transforming growth factor beta-1, tumour necrosis factor alpha, interleukin (IL) 4 (IL-4), and IL-1 receptor antagonist (IL-1ra); and (2) to study possible correlations of these molecules with their respective PRG supernatant treatments. CEs from six horses were cultured for 96 h with L-PRG and P-PRG supernatants at 25 and 50% concentrations, respectively. CE culture media were changed each 48 h and used for determination, by ELISA, of the molecules. L-PRG and P-PRG supernatants at 25 and 50% concentrations influenced the molecular anti-inflammatory profile of CE groups cultured with these substances. 50% L-PRG supernatant produced the most robust pro-inflammatory effects when compared to the CE control group and the CE group treated with the other PRG supernatant concentrations. In general, PRG supernatants induced pro-inflammatory effects in normal CEs.info:eu-repo/semantics/openAccessUniversidad Austral de Chile. Facultad de Ciencias VeterinariasAustral journal of veterinary sciences v.49 n.1 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322017000100104en10.4067/s0719-81322017000100015
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	cartilage cytokines growth factors horse platelet-rich plasma.
spellingShingle	cartilage cytokines growth factors horse platelet-rich plasma. Carmona,Jorge U. Ríos,Diana L. López,Catalina Effects of two platelet-rich gel supernatants at two concentrations on healthy cartilage explants from horses
description	ABSTRACT Platelet-rich plasma is used as a treatment of arthropathies in horses. However, there is lack of in vitro information about the mechanism of action of this substance and its effects on healthy cartilage of these animals. The aims were: (1) to evaluate the effects at 48 and 96 h of two concentrations (25 and 50%) of leukocyte- and platelet-rich gel (L-PRG) and pure platelet rich gel (P-PRG) supernatants on the production/degradation in normal cartilage explants (CEs) of platelet-derived growth factor isoform BB, transforming growth factor beta-1, tumour necrosis factor alpha, interleukin (IL) 4 (IL-4), and IL-1 receptor antagonist (IL-1ra); and (2) to study possible correlations of these molecules with their respective PRG supernatant treatments. CEs from six horses were cultured for 96 h with L-PRG and P-PRG supernatants at 25 and 50% concentrations, respectively. CE culture media were changed each 48 h and used for determination, by ELISA, of the molecules. L-PRG and P-PRG supernatants at 25 and 50% concentrations influenced the molecular anti-inflammatory profile of CE groups cultured with these substances. 50% L-PRG supernatant produced the most robust pro-inflammatory effects when compared to the CE control group and the CE group treated with the other PRG supernatant concentrations. In general, PRG supernatants induced pro-inflammatory effects in normal CEs.
author	Carmona,Jorge U. Ríos,Diana L. López,Catalina
author_facet	Carmona,Jorge U. Ríos,Diana L. López,Catalina
author_sort	Carmona,Jorge U.
title	Effects of two platelet-rich gel supernatants at two concentrations on healthy cartilage explants from horses
title_short	Effects of two platelet-rich gel supernatants at two concentrations on healthy cartilage explants from horses
title_full	Effects of two platelet-rich gel supernatants at two concentrations on healthy cartilage explants from horses
title_fullStr	Effects of two platelet-rich gel supernatants at two concentrations on healthy cartilage explants from horses
title_full_unstemmed	Effects of two platelet-rich gel supernatants at two concentrations on healthy cartilage explants from horses
title_sort	effects of two platelet-rich gel supernatants at two concentrations on healthy cartilage explants from horses
publisher	Universidad Austral de Chile. Facultad de Ciencias Veterinarias
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322017000100104
work_keys_str_mv	AT carmonajorgeu effectsoftwoplateletrichgelsupernatantsattwoconcentrationsonhealthycartilageexplantsfromhorses AT riosdianal effectsoftwoplateletrichgelsupernatantsattwoconcentrationsonhealthycartilageexplantsfromhorses AT lopezcatalina effectsoftwoplateletrichgelsupernatantsattwoconcentrationsonhealthycartilageexplantsfromhorses
_version_	1714207266572337152

Effects of two platelet-rich gel supernatants at two concentrations on healthy cartilage explants from horses

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