Testicular echotexture is not a viable method to indirectly evaluate the spermatogenic parameters in Nelore bulls

ABSTRACT The objective of this study was to evaluate the testicular echotexture of Nelore bulls and to correlate it with spermatogenic parameters. Forty one bulls aged 3-5 years, with body weight of 510.2 ± 21.61 kg were selected for the study. The animals were subjected to ultrasonography, using a...

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Autores principales:	Dias,Wanessa, Faria,Fábio J.C., Fernandes,Carlos A.C., Sampaio,Breno F.B., Santos,Marcelo D., Rodrigues,Walvonvitis B., Silva,Juliana C. Borges, Costa,Deiler S.
Lenguaje:	English
Publicado:	Universidad Austral de Chile. Facultad de Ciencias Veterinarias 2017
Materias:	testicular parenchyma morphometry ultrasonography bovine
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322017000100109
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spelling	oai:scielo:S0719-813220170001001092019-11-28Testicular echotexture is not a viable method to indirectly evaluate the spermatogenic parameters in Nelore bullsDias,WanessaFaria,Fábio J.C.Fernandes,Carlos A.C.Sampaio,Breno F.B.Santos,Marcelo D.Rodrigues,Walvonvitis B.Silva,Juliana C. BorgesCosta,Deiler S. testicular parenchyma morphometry ultrasonography bovine ABSTRACT The objective of this study was to evaluate the testicular echotexture of Nelore bulls and to correlate it with spermatogenic parameters. Forty one bulls aged 3-5 years, with body weight of 510.2 ± 21.61 kg were selected for the study. The animals were subjected to ultrasonography, using a portable device coupled with a linear transducer at a frequency of 7.5 MHz. Immediately afterwards they were castrated and the testicular tissue was treated with Karnovsky fixative. Next, the fragments were embedded in glycol methacrylate and stained with toluidine blue-1% sodium borate solution. The average pixel intensity in the testicular parenchyma was determined for 1,600-mm2 surface sections. The right and left testicles and the area between the two testicles resulted in average pixel intensities of 105.22 ± 13.58, 107.95 ± 12.82, and 106.59 ± 11.43, respectively. The efficiency coefficient of spermatogonial mitosis was 19.74; meiotic index was 2.63, and overall yield of spermatogenesis was 52.0. No correlation (P>0.05) was observed between testicular echotexture and any of the measured morphometric parameters. These results suggest that testicular echotexture was not a viable method for the indirect analysis of cellular population in the seminiferous epithelium, intrinsic spermatogenic performance, or the Sertoli cell index in Nelore bulls.info:eu-repo/semantics/openAccessUniversidad Austral de Chile. Facultad de Ciencias VeterinariasAustral journal of veterinary sciences v.49 n.1 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322017000100109en10.4067/s0719-81322017000100045
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	testicular parenchyma morphometry ultrasonography bovine
spellingShingle	testicular parenchyma morphometry ultrasonography bovine Dias,Wanessa Faria,Fábio J.C. Fernandes,Carlos A.C. Sampaio,Breno F.B. Santos,Marcelo D. Rodrigues,Walvonvitis B. Silva,Juliana C. Borges Costa,Deiler S. Testicular echotexture is not a viable method to indirectly evaluate the spermatogenic parameters in Nelore bulls
description	ABSTRACT The objective of this study was to evaluate the testicular echotexture of Nelore bulls and to correlate it with spermatogenic parameters. Forty one bulls aged 3-5 years, with body weight of 510.2 ± 21.61 kg were selected for the study. The animals were subjected to ultrasonography, using a portable device coupled with a linear transducer at a frequency of 7.5 MHz. Immediately afterwards they were castrated and the testicular tissue was treated with Karnovsky fixative. Next, the fragments were embedded in glycol methacrylate and stained with toluidine blue-1% sodium borate solution. The average pixel intensity in the testicular parenchyma was determined for 1,600-mm2 surface sections. The right and left testicles and the area between the two testicles resulted in average pixel intensities of 105.22 ± 13.58, 107.95 ± 12.82, and 106.59 ± 11.43, respectively. The efficiency coefficient of spermatogonial mitosis was 19.74; meiotic index was 2.63, and overall yield of spermatogenesis was 52.0. No correlation (P>0.05) was observed between testicular echotexture and any of the measured morphometric parameters. These results suggest that testicular echotexture was not a viable method for the indirect analysis of cellular population in the seminiferous epithelium, intrinsic spermatogenic performance, or the Sertoli cell index in Nelore bulls.
author	Dias,Wanessa Faria,Fábio J.C. Fernandes,Carlos A.C. Sampaio,Breno F.B. Santos,Marcelo D. Rodrigues,Walvonvitis B. Silva,Juliana C. Borges Costa,Deiler S.
author_facet	Dias,Wanessa Faria,Fábio J.C. Fernandes,Carlos A.C. Sampaio,Breno F.B. Santos,Marcelo D. Rodrigues,Walvonvitis B. Silva,Juliana C. Borges Costa,Deiler S.
author_sort	Dias,Wanessa
title	Testicular echotexture is not a viable method to indirectly evaluate the spermatogenic parameters in Nelore bulls
title_short	Testicular echotexture is not a viable method to indirectly evaluate the spermatogenic parameters in Nelore bulls
title_full	Testicular echotexture is not a viable method to indirectly evaluate the spermatogenic parameters in Nelore bulls
title_fullStr	Testicular echotexture is not a viable method to indirectly evaluate the spermatogenic parameters in Nelore bulls
title_full_unstemmed	Testicular echotexture is not a viable method to indirectly evaluate the spermatogenic parameters in Nelore bulls
title_sort	testicular echotexture is not a viable method to indirectly evaluate the spermatogenic parameters in nelore bulls
publisher	Universidad Austral de Chile. Facultad de Ciencias Veterinarias
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322017000100109
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Testicular echotexture is not a viable method to indirectly evaluate the spermatogenic parameters in Nelore bulls

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