Glycosaminoglycans (GAGs) determination in healthy and damaged equine articular cartilage

ABSTRACT: The purpose of this study was to establish if there was any difference in the GAGs content between loaded and unloaded surfaces of the joint. Furthermore, the results were compared between macroscopically healthy and damaged joints. Cartilage samples were obtained from two different zones...

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Autores principales:	Adarmes,Héctor, Donders,,Leonardo, Dörner,,Cristóbal, González,,Ema, Galleguillos,Marco
Lenguaje:	English
Publicado:	Universidad Austral de Chile. Facultad de Ciencias Veterinarias 2017
Materias:	equine joint cartilage glycosaminoglycans
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322017000200129
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spelling	oai:scielo:S0719-813220170002001292017-11-14Glycosaminoglycans (GAGs) determination in healthy and damaged equine articular cartilageAdarmes,HéctorDonders,,LeonardoDörner,,CristóbalGonzález,,EmaGalleguillos,Marco equine joint cartilage glycosaminoglycans ABSTRACT: The purpose of this study was to establish if there was any difference in the GAGs content between loaded and unloaded surfaces of the joint. Furthermore, the results were compared between macroscopically healthy and damaged joints. Cartilage samples were obtained from two different zones of the equine metacarpophalangeal joint (metacarpal condyles). Samples were collected from the loaded surface of macroscopically healthy joints (N1; n=10) and from macroscopically damaged cartilage (P1; n=10). Additionally, cartilage samples were collected from unloaded areas at the most dorso-proximal zone of the joint in macroscopically healthy joints (N2; n=10) and from the macroscopically pathological joints but without damaged cartilage on the site of sampling (P2; n=10). The GAGs were extracted from 100 mg of cartilage of each sample and quantified through the safranine - O method that measured the total anionic charges, and through the carbazole method that measured the uronic acid content. Both methods measured the GAGs content, showing no differences between intra-joint zones (1 and 2), but when the GAGs content was compared between healthy and pathological joints, both methods showed a significantly decreased GAGs content in the damaged joints (1 and 2). These results show that the whole articular cartilage could be affected in a chronic pathological process and is not only a local process occurring in the macroscopically damaged cartilage associated with the loaded area.info:eu-repo/semantics/openAccessUniversidad Austral de Chile. Facultad de Ciencias VeterinariasAustral journal of veterinary sciences v.49 n.2 20172017-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322017000200129en10.4067/S0719-81322017000200129
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	equine joint cartilage glycosaminoglycans
spellingShingle	equine joint cartilage glycosaminoglycans Adarmes,Héctor Donders,,Leonardo Dörner,,Cristóbal González,,Ema Galleguillos,Marco Glycosaminoglycans (GAGs) determination in healthy and damaged equine articular cartilage
description	ABSTRACT: The purpose of this study was to establish if there was any difference in the GAGs content between loaded and unloaded surfaces of the joint. Furthermore, the results were compared between macroscopically healthy and damaged joints. Cartilage samples were obtained from two different zones of the equine metacarpophalangeal joint (metacarpal condyles). Samples were collected from the loaded surface of macroscopically healthy joints (N1; n=10) and from macroscopically damaged cartilage (P1; n=10). Additionally, cartilage samples were collected from unloaded areas at the most dorso-proximal zone of the joint in macroscopically healthy joints (N2; n=10) and from the macroscopically pathological joints but without damaged cartilage on the site of sampling (P2; n=10). The GAGs were extracted from 100 mg of cartilage of each sample and quantified through the safranine - O method that measured the total anionic charges, and through the carbazole method that measured the uronic acid content. Both methods measured the GAGs content, showing no differences between intra-joint zones (1 and 2), but when the GAGs content was compared between healthy and pathological joints, both methods showed a significantly decreased GAGs content in the damaged joints (1 and 2). These results show that the whole articular cartilage could be affected in a chronic pathological process and is not only a local process occurring in the macroscopically damaged cartilage associated with the loaded area.
author	Adarmes,Héctor Donders,,Leonardo Dörner,,Cristóbal González,,Ema Galleguillos,Marco
author_facet	Adarmes,Héctor Donders,,Leonardo Dörner,,Cristóbal González,,Ema Galleguillos,Marco
author_sort	Adarmes,Héctor
title	Glycosaminoglycans (GAGs) determination in healthy and damaged equine articular cartilage
title_short	Glycosaminoglycans (GAGs) determination in healthy and damaged equine articular cartilage
title_full	Glycosaminoglycans (GAGs) determination in healthy and damaged equine articular cartilage
title_fullStr	Glycosaminoglycans (GAGs) determination in healthy and damaged equine articular cartilage
title_full_unstemmed	Glycosaminoglycans (GAGs) determination in healthy and damaged equine articular cartilage
title_sort	glycosaminoglycans (gags) determination in healthy and damaged equine articular cartilage
publisher	Universidad Austral de Chile. Facultad de Ciencias Veterinarias
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322017000200129
work_keys_str_mv	AT adarmeshector glycosaminoglycansgagsdeterminationinhealthyanddamagedequinearticularcartilage AT dondersleonardo glycosaminoglycansgagsdeterminationinhealthyanddamagedequinearticularcartilage AT dornercristobal glycosaminoglycansgagsdeterminationinhealthyanddamagedequinearticularcartilage AT gonzalezema glycosaminoglycansgagsdeterminationinhealthyanddamagedequinearticularcartilage AT galleguillosmarco glycosaminoglycansgagsdeterminationinhealthyanddamagedequinearticularcartilage
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Glycosaminoglycans (GAGs) determination in healthy and damaged equine articular cartilage

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