Absence of molecular evidence of Leptospira spp. in urine samples collected from rodents captured in Yucatán, México

ABSTRACT. Leptospira spp. is a spirochete bacteria, causal agent of leptospirosis, zoonotic disease endemic in México that represents a serious public health and veterinary problem. Rodents are recognised as the most important reservoirs of this bacteria, which is transmitted mainly through direct o...

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Autores principales:	Torres-Castro,Marco A., Cruz-Camargo,Bayron E., Medina-Pinto,Rodrigo, Moguel-Lehmer,Carlos, Arcila-Fuentes,William, Medina,Rolando, Ortiz-Esquivel,José, López-Ávila,Armando, Noh-Pech,Henry R., Panti-May,Jesús A., Rodríguez-Vivas,Roger I., Puerto,Fernando I.
Lenguaje:	English
Publicado:	Universidad Austral de Chile. Facultad de Ciencias Veterinarias 2017
Materias:	urine rodents Leptospira spp. Yucatán México
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322017000300195
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spelling	oai:scielo:S0719-813220170003001952017-11-29Absence of molecular evidence of Leptospira spp. in urine samples collected from rodents captured in Yucatán, MéxicoTorres-Castro,Marco A.Cruz-Camargo,Bayron E.Medina-Pinto,RodrigoMoguel-Lehmer,CarlosArcila-Fuentes,WilliamMedina,RolandoOrtiz-Esquivel,JoséLópez-Ávila,ArmandoNoh-Pech,Henry R.Panti-May,Jesús A.Rodríguez-Vivas,Roger I.Puerto,Fernando I. urine rodents Leptospira spp. Yucatán México ABSTRACT. Leptospira spp. is a spirochete bacteria, causal agent of leptospirosis, zoonotic disease endemic in México that represents a serious public health and veterinary problem. Rodents are recognised as the most important reservoirs of this bacteria, which is transmitted mainly through direct or indirect contact with the Leptospira spp. excreted in the urine of infected individuals. The aim of this study was to evaluate the circulation of Leptospira spp. in urine samples of wild and synanthropic rodents from Yucatán, México. Eighty-four rodents were captured in the community of Cenotillo, Yucatán. Twenty-six urine samples were collected from the bladder and were used in the total DNA extraction. The identification of Leptospira spp. was intended through the polymerase chain reaction test in its endpoint variant. No evidence of Leptospira spp. was found in the urine samples. It is necessary to use other tissues for the identification of Leptospira spp., before concluding that the rodents used in the present study are not reservoirs of this bacteria.info:eu-repo/semantics/openAccessUniversidad Austral de Chile. Facultad de Ciencias VeterinariasAustral journal of veterinary sciences v.49 n.3 20172017-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322017000300195en10.4067/S0719-81322017000300195
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	urine rodents Leptospira spp. Yucatán México
spellingShingle	urine rodents Leptospira spp. Yucatán México Torres-Castro,Marco A. Cruz-Camargo,Bayron E. Medina-Pinto,Rodrigo Moguel-Lehmer,Carlos Arcila-Fuentes,William Medina,Rolando Ortiz-Esquivel,José López-Ávila,Armando Noh-Pech,Henry R. Panti-May,Jesús A. Rodríguez-Vivas,Roger I. Puerto,Fernando I. Absence of molecular evidence of Leptospira spp. in urine samples collected from rodents captured in Yucatán, México
description	ABSTRACT. Leptospira spp. is a spirochete bacteria, causal agent of leptospirosis, zoonotic disease endemic in México that represents a serious public health and veterinary problem. Rodents are recognised as the most important reservoirs of this bacteria, which is transmitted mainly through direct or indirect contact with the Leptospira spp. excreted in the urine of infected individuals. The aim of this study was to evaluate the circulation of Leptospira spp. in urine samples of wild and synanthropic rodents from Yucatán, México. Eighty-four rodents were captured in the community of Cenotillo, Yucatán. Twenty-six urine samples were collected from the bladder and were used in the total DNA extraction. The identification of Leptospira spp. was intended through the polymerase chain reaction test in its endpoint variant. No evidence of Leptospira spp. was found in the urine samples. It is necessary to use other tissues for the identification of Leptospira spp., before concluding that the rodents used in the present study are not reservoirs of this bacteria.
author	Torres-Castro,Marco A. Cruz-Camargo,Bayron E. Medina-Pinto,Rodrigo Moguel-Lehmer,Carlos Arcila-Fuentes,William Medina,Rolando Ortiz-Esquivel,José López-Ávila,Armando Noh-Pech,Henry R. Panti-May,Jesús A. Rodríguez-Vivas,Roger I. Puerto,Fernando I.
author_facet	Torres-Castro,Marco A. Cruz-Camargo,Bayron E. Medina-Pinto,Rodrigo Moguel-Lehmer,Carlos Arcila-Fuentes,William Medina,Rolando Ortiz-Esquivel,José López-Ávila,Armando Noh-Pech,Henry R. Panti-May,Jesús A. Rodríguez-Vivas,Roger I. Puerto,Fernando I.
author_sort	Torres-Castro,Marco A.
title	Absence of molecular evidence of Leptospira spp. in urine samples collected from rodents captured in Yucatán, México
title_short	Absence of molecular evidence of Leptospira spp. in urine samples collected from rodents captured in Yucatán, México
title_full	Absence of molecular evidence of Leptospira spp. in urine samples collected from rodents captured in Yucatán, México
title_fullStr	Absence of molecular evidence of Leptospira spp. in urine samples collected from rodents captured in Yucatán, México
title_full_unstemmed	Absence of molecular evidence of Leptospira spp. in urine samples collected from rodents captured in Yucatán, México
title_sort	absence of molecular evidence of leptospira spp. in urine samples collected from rodents captured in yucatán, méxico
publisher	Universidad Austral de Chile. Facultad de Ciencias Veterinarias
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322017000300195
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_version_	1714207271352795136

Absence of molecular evidence of Leptospira spp. in urine samples collected from rodents captured in Yucatán, México

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