Identification of non-tuberculous mycobacteria isolated from opossum ( Didelphis virginiana ) lymph nodes and characterisation of lesions

ABSTRACT. The aim of this study was to investigate the presence of NTM in the lymph nodes of opossums (D. virginiana) and to characterise the microscopic changes in affected tissue. Retropharyngeal and tracheobronchial lymph nodes were collected postmortem from 18 opossums in the state of Colima, Me...

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Autores principales:	García,Luis J., González,David A., Prado,Omar F., Macedo,Julio R., Ramírez,R, Martínez,J, Barrios,Hugo B., López,A, Constantino,F
Lenguaje:	English
Publicado:	Universidad Austral de Chile. Facultad de Ciencias Veterinarias 2017
Materias:	mycobacteria tuberculosis opossums lesions
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322017000300199
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spelling	oai:scielo:S0719-813220170003001992017-11-29Identification of non-tuberculous mycobacteria isolated from opossum ( Didelphis virginiana ) lymph nodes and characterisation of lesionsGarcía,Luis J.González,David A.Prado,Omar F.Macedo,Julio R.Ramírez,RMartínez,JBarrios,Hugo B.López,AConstantino,F mycobacteria tuberculosis opossums lesions ABSTRACT. The aim of this study was to investigate the presence of NTM in the lymph nodes of opossums (D. virginiana) and to characterise the microscopic changes in affected tissue. Retropharyngeal and tracheobronchial lymph nodes were collected postmortem from 18 opossums in the state of Colima, Mexico in 2013. The lymph nodes were also cultured for mycobacterial organisms and processed for histopathological examination. Bacteriological cultures yielded 5/18X100 (28%) isolates of NTM, which were subsequently identified as M. terrae, M. szulgai, M. gastri and M. asiaticum. Microscopic examination of the affected nodes revealed a necrotic granulomatous lymphadenitis (3/60%) composed of histiocytes, epithelioid cells and giant cells with intralesional alcoholresistant acid bacteria. An association between the sex of the opossum and the presence of NTM was observed. To our knowledge, this is the first report of NTM isolation in opossums with granulomatous lymphadenitis in Mexico.info:eu-repo/semantics/openAccessUniversidad Austral de Chile. Facultad de Ciencias VeterinariasAustral journal of veterinary sciences v.49 n.3 20172017-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322017000300199en10.4067/S0719-81322017000300199
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	mycobacteria tuberculosis opossums lesions
spellingShingle	mycobacteria tuberculosis opossums lesions García,Luis J. González,David A. Prado,Omar F. Macedo,Julio R. Ramírez,R Martínez,J Barrios,Hugo B. López,A Constantino,F Identification of non-tuberculous mycobacteria isolated from opossum ( Didelphis virginiana ) lymph nodes and characterisation of lesions
description	ABSTRACT. The aim of this study was to investigate the presence of NTM in the lymph nodes of opossums (D. virginiana) and to characterise the microscopic changes in affected tissue. Retropharyngeal and tracheobronchial lymph nodes were collected postmortem from 18 opossums in the state of Colima, Mexico in 2013. The lymph nodes were also cultured for mycobacterial organisms and processed for histopathological examination. Bacteriological cultures yielded 5/18X100 (28%) isolates of NTM, which were subsequently identified as M. terrae, M. szulgai, M. gastri and M. asiaticum. Microscopic examination of the affected nodes revealed a necrotic granulomatous lymphadenitis (3/60%) composed of histiocytes, epithelioid cells and giant cells with intralesional alcoholresistant acid bacteria. An association between the sex of the opossum and the presence of NTM was observed. To our knowledge, this is the first report of NTM isolation in opossums with granulomatous lymphadenitis in Mexico.
author	García,Luis J. González,David A. Prado,Omar F. Macedo,Julio R. Ramírez,R Martínez,J Barrios,Hugo B. López,A Constantino,F
author_facet	García,Luis J. González,David A. Prado,Omar F. Macedo,Julio R. Ramírez,R Martínez,J Barrios,Hugo B. López,A Constantino,F
author_sort	García,Luis J.
title	Identification of non-tuberculous mycobacteria isolated from opossum ( Didelphis virginiana ) lymph nodes and characterisation of lesions
title_short	Identification of non-tuberculous mycobacteria isolated from opossum ( Didelphis virginiana ) lymph nodes and characterisation of lesions
title_full	Identification of non-tuberculous mycobacteria isolated from opossum ( Didelphis virginiana ) lymph nodes and characterisation of lesions
title_fullStr	Identification of non-tuberculous mycobacteria isolated from opossum ( Didelphis virginiana ) lymph nodes and characterisation of lesions
title_full_unstemmed	Identification of non-tuberculous mycobacteria isolated from opossum ( Didelphis virginiana ) lymph nodes and characterisation of lesions
title_sort	identification of non-tuberculous mycobacteria isolated from opossum ( didelphis virginiana ) lymph nodes and characterisation of lesions
publisher	Universidad Austral de Chile. Facultad de Ciencias Veterinarias
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322017000300199
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Identification of non-tuberculous mycobacteria isolated from opossum ( Didelphis virginiana ) lymph nodes and characterisation of lesions

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