Comparison of common tests performance for Mycobacterium bovis infection diagnosis in low prevalence dairy cattle herds of southern Chile

ABSTRACT: The aim of the present study was to assess the performance of routinely used diagnostic tests to detect M. bovis infection in cattle populations with very low infection prevalence. A cross-sectional study was performed using 609 animals from 17 herds that were sampled for blood interferon,...

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Autores principales:	Raffo,Eduardo, Monti,Gustavo, Salgado,Miguel
Lenguaje:	English
Publicado:	Universidad Austral de Chile. Facultad de Ciencias Veterinarias 2018
Materias:	bovine tuberculosis prevalence diagnose
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322018000100109
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spelling	oai:scielo:S0719-813220180001001092018-01-08Comparison of common tests performance for Mycobacterium bovis infection diagnosis in low prevalence dairy cattle herds of southern ChileRaffo,EduardoMonti,GustavoSalgado,Miguel bovine tuberculosis prevalence diagnose ABSTRACT: The aim of the present study was to assess the performance of routinely used diagnostic tests to detect M. bovis infection in cattle populations with very low infection prevalence. A cross-sectional study was performed using 609 animals from 17 herds that were sampled for blood interferon, and tested with the caudal fold tuberculin test (CFT). It was planned that animals which showed CFT positive results were sent to the slaughterhouse for post mortem confirmation testing. However, not all the animals considered in the initial sampling were culled. Paired population proportions of positive results for the different diagnostic tools were compared. Apparent sensitivity and specificity was also estimated. Overall, intradermal tuberculin based diagnostics showed better performance in comparison to interferon based test, even though interferon was slightly more specific. From these results, it may be suggested that specific cut-off lines for interferon based techniques for certain areas with particular low bTB prevalence should be considered, as well as the modification of more specific antigens and the use of better combinations of PPDs if in vitro testing is intended must be taken into consideration. The uncertain role of interference bacteria that could be affecting the results of the in vitro analysis must be considered, and perhaps this could explain the apparent lack of consensus of the results obtained in Chile versus the ones obtained elsewhere. More studies must be performed in order to assess this issue.info:eu-repo/semantics/openAccessUniversidad Austral de Chile. Facultad de Ciencias VeterinariasAustral journal of veterinary sciences v.50 n.1 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322018000100109en10.4067/S0719-81322018000100109
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	bovine tuberculosis prevalence diagnose
spellingShingle	bovine tuberculosis prevalence diagnose Raffo,Eduardo Monti,Gustavo Salgado,Miguel Comparison of common tests performance for Mycobacterium bovis infection diagnosis in low prevalence dairy cattle herds of southern Chile
description	ABSTRACT: The aim of the present study was to assess the performance of routinely used diagnostic tests to detect M. bovis infection in cattle populations with very low infection prevalence. A cross-sectional study was performed using 609 animals from 17 herds that were sampled for blood interferon, and tested with the caudal fold tuberculin test (CFT). It was planned that animals which showed CFT positive results were sent to the slaughterhouse for post mortem confirmation testing. However, not all the animals considered in the initial sampling were culled. Paired population proportions of positive results for the different diagnostic tools were compared. Apparent sensitivity and specificity was also estimated. Overall, intradermal tuberculin based diagnostics showed better performance in comparison to interferon based test, even though interferon was slightly more specific. From these results, it may be suggested that specific cut-off lines for interferon based techniques for certain areas with particular low bTB prevalence should be considered, as well as the modification of more specific antigens and the use of better combinations of PPDs if in vitro testing is intended must be taken into consideration. The uncertain role of interference bacteria that could be affecting the results of the in vitro analysis must be considered, and perhaps this could explain the apparent lack of consensus of the results obtained in Chile versus the ones obtained elsewhere. More studies must be performed in order to assess this issue.
author	Raffo,Eduardo Monti,Gustavo Salgado,Miguel
author_facet	Raffo,Eduardo Monti,Gustavo Salgado,Miguel
author_sort	Raffo,Eduardo
title	Comparison of common tests performance for Mycobacterium bovis infection diagnosis in low prevalence dairy cattle herds of southern Chile
title_short	Comparison of common tests performance for Mycobacterium bovis infection diagnosis in low prevalence dairy cattle herds of southern Chile
title_full	Comparison of common tests performance for Mycobacterium bovis infection diagnosis in low prevalence dairy cattle herds of southern Chile
title_fullStr	Comparison of common tests performance for Mycobacterium bovis infection diagnosis in low prevalence dairy cattle herds of southern Chile
title_full_unstemmed	Comparison of common tests performance for Mycobacterium bovis infection diagnosis in low prevalence dairy cattle herds of southern Chile
title_sort	comparison of common tests performance for mycobacterium bovis infection diagnosis in low prevalence dairy cattle herds of southern chile
publisher	Universidad Austral de Chile. Facultad de Ciencias Veterinarias
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322018000100109
work_keys_str_mv	AT raffoeduardo comparisonofcommontestsperformanceformycobacteriumbovisinfectiondiagnosisinlowprevalencedairycattleherdsofsouthernchile AT montigustavo comparisonofcommontestsperformanceformycobacteriumbovisinfectiondiagnosisinlowprevalencedairycattleherdsofsouthernchile AT salgadomiguel comparisonofcommontestsperformanceformycobacteriumbovisinfectiondiagnosisinlowprevalencedairycattleherdsofsouthernchile
_version_	1714207273492938752

Comparison of common tests performance for Mycobacterium bovis infection diagnosis in low prevalence dairy cattle herds of southern Chile

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